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Biomedical subjects

G Asano

Publications and source records attributed to G Asano.

At least 145 records · Page 8Linked to original sources
PubMed1989

In situ localization of pepsinogens I and II mRNA in human gastric mucosa.

Localization of pepsinogens I and II mRNA in the human gastric mucosa was investigated by an in situ hybridization method using digoxigenin-labeled cDNA probes. Gastric fundic mucosa from healthy volunteers, which was stained with digoxigenin-labeled pepsinogens I and II cDNA probes, showed positive staining in the cytoplasm of both chief cells and mucous neck cells. In contrast, gastric antral mucosa stained with the pepsinogen I cDNA probe showed no positive reaction in the surface mucous cells or pyloric glands. On the other hand, the pyloric glands were stained positively with the pepsinogen II cDNA probe and the staining appeared to be identical to that obtained with the antipepsinogens I and II monoclonal antibodies using the avidin-biotin-peroxidase complex technique. These results are consistent with those of previous studies that have employed immunochemical and immunohistochemical techniques.

DNA↗
PubMed1989

[An investigation of sudden cardiac death in apparently healthy young men by annual health examination].

We have clinically compared the health records of 58 apparently healthy men who succumbed to sudden cardiac death (SCD) against the same number of control subjects (CS) with similar physical and social backgrounds. SCD, for our purpose, was defined as a death that occurred from a non-traumatic cause within one hour after the SCD onset. The clinical examination of our SCD cases consisted of evaluating the findings derived from their health records covering a span of 13 years such as their ECGs, the blood pressure data, chest x-rays, serum chemical analysis, and their obesity indexes. Heart weights were estimated from their ECGs by the quantification of a multivariate analysis. Of the total SCD cases studied, eleven hearts also were examined pathologically. With respect to the physical conditions of the SCD cases and the CS cases, the systolic blood pressure in the SCD cases was found to be higher, whereas no significant variances in the diastolic values were found, and both the systolic and diastolic values were within normal ranges in the two groups. A greater number of hearts weighing more than 420 g were found in the SCD group. Similarly the frequency of premature contraction and the serum cholesterol level was higher. No further appreciable differences, however, were found. Myocardial cell hypertrophy, degeneration, and interstitial edema were observed in all eleven hearts. Our data have revealed that the greater the heart weight, the greater the risk of succumbing to SCD. The risk becomes more evident if the systolic and cholesterol levels are also found to be high.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

Synthesis of prolyl 4-hydroxylase by cultured chicken corneal cells.

Prolyl hydroxylase (EC 1.14.11.2) synthesis was examined in the cultured corneas of 17-day-old chick embryos which were cultured in F-12 medium supplemented with fetal calf serum. The amount of prolyl hydroxylase in cells was measured by immunoelectrophoresis using antibodies directed against this enzyme. The results indicated that the amounts of prolyl hydroxylase depended on cell density. The cells were harvested at the early-log phase and plated at high cell density to mimic the culture condition of cells at the stationary phase (crowding). The amount of 35S-prolyl hydroxylase indicated a two-fold increase in the rate of prolyl hydroxylase synthesis after crowding. By use of 32P-labeled cDNA for the beta-subunit of prolyl hydroxylase a proportionate increase of the mRNA was noted in accordance with the increase in the rate of prolyl hydroxylase synthesis, suggesting that this enzyme synthesis is regulated at the transcriptional level.

Animals↗
PubMed1989

[Metastasis into the paracystium of urinary bladder carcinoma--the rate position and morphology of metastasis].

Twenty-three patients, who underwent radical cystectomy and pelvic lymph node dissection for bladder carcinoma from November 1985 to November 1987, were studied pathologically. We evaluated the rate, position and morphology of metastasis into the neurovascular corridor or paracystium. The stages of the twenty-three patients were G1 1, G2 10, G3 12 and pT1a 1, pT1b 4, pT2 8, pT3a 2, pT3b 7, pT4 1. Of the 23 specimens, 22 (95%) were found to contain metastasis in the connective tissue of the paracystium. Lymph node metastasis was recognized in four patients (17%). Metastasis in connective tissue around the iliac lymph node was recognized in 5 patients (22%). Of these 5 patients, 3 were free from lymph node metastasis. The morphological pattern of metastasis was ranged from a few tumor cells to massive tumor cells with proliferation of fibroblasts. The existence of metastasis in the paracystium should be evaluated morphologically for urinary bladder carcinoma.

Adult↗
PubMed1989

Localization of type III procollagen mRNA in areas of liver fibrosis by in situ hybridization.

Localization of type III procollagen mRNA in human liver was studied by in situ hybridization using human alpha 1(III) procollagen cDNA. Frozen and paraformaldehyde-fixed sections of biopsied human liver from patients with chronic hepatitis and cirrhosis were examined using the digoxigenin-labeled cDNA probe. Localization of the type III procollagen mRNA was demonstrated not only in the cytoplasm of mesenchymal cells but also in a large number of hepatocytes, in proportion to the extent of fibrosis. These results suggest that hepatocytes play an important role in fibrogenesis in the liver.

DNA Probes↗
PubMed1989

[Effect of adriamycin on cell cycle by bromodeoxyuridine-labeling method].

We studied the effect of adriamycin on DNA synthesizing cells from the view point of cell cycle. DNA synthesizing cells were marked by bromodeoxyuridine, which is a known marker for such cells, before administration of adriamycin. Then, the level of this marker was sequentially measured by flow cytometry. At the low concentration (0.01 micrograms/ml), adriamycin caused delay of the shifting time to the S phase 16 hours after administration. The marked cells were accumulated at the G2M phase at a moderate concentration (0.1 micrograms/ml), and blocked at the S phase at the higher concentration (1.0 micrograms/ml).

Bromodeoxyuridine↗
PubMed1988

Neovascularization of pericellular fibrosis in alcoholic liver disease.

The relationship between sinusoidal capillarization and pericellular fibrosis was studied in 29 specimens of human liver from patients with alcoholic liver disease. Immunohistochemically, factor VIII-related antigen was not observed in the normal sinusoidal lining cells, but was localized in the capillaries which proliferated in the pericellular fibrotic region. Fibronectin was localized in the proliferated endoplasmic reticulum of newly formed vascular endothelial cells and hepatocytes with the development of pericellular fibrosis, laminin became apparent in the plasmalemmal vesicles of capillary endothelial cells and pericytes and in the endoplasmic reticulum of mesenchymal cells in Disse's space. From these results, it was revealed that pericellular fibrosis was closely related to sinusoidal capillarization. Furthermore, it is suggested that this sinusoidal capillarization may be caused by neovascularization, and that the extracellular matrix produced by endothelial cells, hepatocytes and mesenchymal cells may promote this process.

Antigens↗
PubMed1987

Immunohistochemical study of intracellular estradiol in human gastric cancer.

Tissues from primary human gastric cancers were examined for intracellular estradiol (E2) by using the avidin-biotin-peroxidase complex (ABC) immunohistochemical method on formalin-fixed paraffin-embedded sections. Reaction products of E2 were located only in the cytoplasm of the cancer cells, and not detected in noncancerous gastric epithelium. E2-positive tissues were found in 23 (44.2%) of 52 male patients, seven (20.6%) of 34 female patients and a total of 30 (34.9%) of 86 patients. In male patients, E2-positive cases occurred without age distinction. In female patients, however, E2 was not found in patients in older age groups, especially patients in the postmenopausal state. Microscopically, E2 was found frequently in intestinal type of cancers in male patients and in cancer with scirrhous growth pattern, in female patients. This is the first report of the demonstration of E2 in gastric cancer. The findings suggest that hormonal factors are involved in gastric cancer, and that the cancers contain endocrinic characteristics.

Adult↗
PubMed1987

Role of glycosaminoglycan and fibronectin in endothelial cell growth.

This study was undertaken to clarify the biological significance of fibronectin and GAG synthesized in human endothelium. The synthesis of GAG and fibronectin was proved morphologically and biochemically in endothelial cells. The conditioned media of fibroblasts and smooth muscle cells have promoted endothelial cell proliferation and production of extracellular matrix in the endothelium. These results suggest that the extracellular matrix which is composed of fibronectin and GAG may play an important role in endothelial cell regeneration.

Cell Division↗
PubMed1987

[A case of B cell malignant lymphoma with in the liver and brain metastasis in Hashimoto's disease].

We have reported a case of malignant lymphoma in associated with Hashimoto's disease arising in the right lobe of thyroid of a 57-year-old woman. The pre-operative aspirated biopsy was valuable for diagnosis Immuno-histochemical observations revealed the localization of anti B cell antibody in the cell surface of lymphoma cells and anti thyroglobulin antibody in intactly follicular epithelium within the lymphoma tissue. This lymphoma was diagnosed as diffuse, medium cell and B cell type. Radiation and chemotherapy were performed for multiple metastasis of liver and brain, but this case had died of DIC and diffuse hyaline membrane disease of lung.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

Induction of intestinal metaplasia in rats by N-ethyl-N'-nitro-N-nitrosoguanidine but not by sodium hydroxide.

Intestinal metaplasia (IM) in the glandular stomach of male Wistar rats induced by oral administration of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) and/or intubation of 0.1N sodium hydroxide (NaOH) was studied as follows. Experiment I, sequential study: Rats in group I were given 100 micrograms/ml ENNG in drinking water for 12 weeks. Rats in group II were given 5 ml of 0.1N NaOH by gastric intubation once a week for 12 weeks. Group III control rats were given tap water. Rats were killed from week 1 until week 69 sequentially. IM was first found at week 26 in group I and at week 58 in groups II and III, its incidence being significantly higher in group I than in the other two groups (P less than 0.01), but without any difference between group II and group III. Experiment II, two-stage carcinogenesis: Rats in groups I and II were treated in the same way as in experiment I, while rats in group III were given 100 micrograms/ml ENNG for 12 weeks, followed by 0.1N NaOH once a week for 12 weeks intragastrically. All rats were killed at week 56. The numbers of metaplastic glands in groups I and III were higher than in group II. Gastric carcinomas were induced in all groups of rats treated with ENNG. The results of these two experiments show that IM is effectively induced by a carcinogen but is not enhanced by regeneration induced by alkaline treatment.

Aging↗