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Biomedical subjects

G Adam

Publications and source records attributed to G Adam.

At least 289 records · Page 16Linked to original sources
PubMed1990

Tissue response of the biliary and digestive system of rabbits after MTBE infusion into the gallbladder.

We evaluated the tissue response of the biliary and digestive system after Methyl-tert-Butyl-Ether (MTBE) gallbladder infusion in 32 rabbits. After laparotomy, MTBE (5-11 ml) was infused into the gallbladder for eight hours. Animals were sacrificed after eight hours or after two months. Control animals received saline solution infusion into the gallbladder. Afterwards the gallbladder, the common bile duct, the liver, the pancreas and the duodenum were examined histologically. All animals receiving MTBE had different degrees of gallbladder necrosis, common bile duct necrosis and necrosis of intrahepatic bile ducts. After two months, scar formation and a hyperplastic cholecystitis were observed. Control animals did not have comparable tissue reactions; only small areas of necrosis in the gallbladder and the common bile duct occurred after eight hours. The gallbladder, common bile duct and liver remained unchanged in those animals which survived two months. Although the results of this animal study cannot be directly transferred to humans, the data suggest that MTBE should be used in gallstone therapy with caution, and that if it is used, a well-controlled follow-up of these patients is necessary.

Animals↗
PubMed1990

Vascular tissue ablation by an erbium-YAG laser: a fiber-transmittable pulsed laser in the infrared range.

Vascular tissue ablation by an erbium:YAG laser of 2,940 nm was tested in-vitro with the application of increasing energies from 20 to 80 mJ per pulse. Effective ablation of normal vascular tissue, lipomatous atheromas and calcified plaques was achieved. Penetration depth was considerably diminished in the presence of calcifications. The lasered craters showed smooth surfaces and were regularly shaped. The solid state erbium:YAG, which is transmittable by fluoride fibers, offers theoretical advantages for laser angioplasty.

Aortic Diseases↗
PubMed1990

Effects of (22S,23S)-Homobrassinolide and Related Compounds on Membrane Potential and Transport of Egeria Leaf Cells.

(22S,23S)-Homobrassinolide was tested for its effect on the electric cell potential, proton extrusion, ferricyanide reduction, and amino acid and sucrose uptake of leaves of Egeria densa Planchon. In the light, (22S,23S)-homobrassinolide and its derivative, 2alpha-3alpha-dihydroxy-5alpha-stigmast-22-en-6-one, were similar to each other and similar to fusicoccin in causing hyperpolarization and proton extrusion, whereas stigmasterol was less effective. In darkness, the three sterols showed comparable effects. (22S,23S)-Homobrassinolide slightly stimulated ferricyanide reduction and promoted uptake of sucrose and alpha-aminoisobutyric acid. The results are compatible with a stimulation of an electrogenic proton pump mechanism at the plasmalemma by (22S,23S)-homobrassinolide.

Journal Article↗
PubMed1990

Very fast purification of ornithine decarboxylase with high yield from mouse kidney and generation of a monoclonal antibody.

Based on methods for ornithine-decarboxylase purification published previously we developed an improved procedure for purification of the enzyme from the kidneys of testosterone-treated NMRI mice. Advantages of the new procedure are, that inactivation of the enzyme during purification is largely reduced by fast methods for purification and by the use of proteinase inhibitors. That way we got pure ornithine decarboxylase within 60 h with a yield of about 70%. A part of the highly purified ornithine decarboxylase was used for the generation of monoclonal antibodies.

Animals↗
PubMed1990

[Integrated pedagogic-therapeutic process in inpatient group therapy work with behaviorally disordered adolescents].

This article describes a group project involving educational-therapeutic work on an inpatient basis with behaviourally disturbed adolescents. It shows how the integration of education and therapy, which is considered so necessary at the inpatient level, is achieved in group therapy and it describes as well the institutional framework within which the group project is carried out, as well as the type of practical experience with behaviourally disturbed adolescents which preceded the development and application of the group therapy work. The initial conditions, aims and the development process of the group therapy which took place over a period of two years are presented and four development phases are identified and explained. The article ends with observations concerning the self-evaluation of the group leaders, the general experience gained in the setting up of educational-therapeutic group work with behaviourally disturbed adolescents and a review of the preliminary results of the group project.

Adolescent↗
PubMed1989

Haplotypes in cystic fibrosis patients with or without pancreatic insufficiency from four European populations.

We examined the allele and haplotype frequencies of five polymorphic DNA markers in 355 European cystic fibrosis (CF) patients (from Belgium, the German Democratic Republic, Greece, and Italy) who were divided into two groups according to whether they were or not taking supplementary pancreatic enzymes. The level of linkage disequilibrium between each polymorphism and the CF mutation varied among the different populations; there was no significant association between KM.19 and CF in the Greek population. The distributions of alleles and haplotypes derived from the polymorphisms revealed by probes KM.19 and XV.2c were always different in patients with or without pancreatic insufficiency (PI) in all the populations studied. In particular, among 32 patients without PI, only 9 (or 28%) were homozygous for the KM.19-XV.2c = 2-1 haplotype (which was present in 73% of all the CF chromosomes in our sample) compared to 162 of 252 patients (or 64%) with PI. These findings are consistent with the hypothesis that pancreatic insufficiency or sufficiency may be determined by different mutations at the CF locus.

Alleles↗
PubMed1989

[NMR tomography of the cartilage structures of the knee joint with 3D-volume imaging combined with a rapid optical-imaging computer].

MRI is of increasing importance as a noninvasive method for the investigation of abnormalities in the knee. 2-D spin-echo sequences were compared with 3-D gradient-echo sequence using normal and cadaver knee joints. 3-D imaging using a fast computer was found to provide significantly improved diagnostic information concerning the cartilage when compared with a 2-D spin-echo techniques. The important advantages are: sections of less than 1 mm., reconstruction in any required plane, which can be related to the complex anatomy of the knee joint, and very good distinction between intra-articular fluid, fibrocartilage and hyaline cartilage.

Cadaver↗
PubMed1989

Gratuity for doctors and medical ethics.

The habit of giving a gratuity became so frequent at the end of the 1950's that counter-measures were enacted. These have been completely ineffective. Although granting and accepting gratuities is forbidden by law, the wages of doctors have been fixed since 1954, for so long that accepting gratuities has come to be considered part of the wages, even in semi-official comments and in the media. The author is of the opinion that, in view of this anomaly, a fundamental transformation of the health provision system (sickness insurance) is needed. The doctor should be paid according to the number of patients in his or her charge, instead of the mere number of the sick. By transforming the provision of medical care in this way, the giving and accepting of gratuities for doctors could be terminated, not only in law but also in the economy.

Ethics, Medical↗
PubMed1989

Effect of partial agonist activity on the side effects of beta-blockade in patients with chronic stable angina.

Some side effects of the beta 1-adrenoceptor blocker atenolol may result from depression of cardiac output at rest. They may, therefore, be reduced by the use of drugs with beta 1-partial agonist activity, such as epanolol. We compared once-daily atenolol 100mg and epanolol 200mg in 20 patients reporting side effects while taking atenolol for chronic stable angina. A double-dummy, double-blind, crossover design was used to assess side effects by use of visual analogue scales and interviews, and antianginal efficacy by treadmill exercise tests and diary cards. In a comparison with atenolol, no significant differences in exercise time (686 +/- 11 seconds vs 685 +/- 10 seconds, maximum ST depression (1.02 +/- 0.09mm vs 1.07 +/- 0.08mm), time to 1mm ST depression (8.4 +/- 1.9 minutes vs 9.0 +/- 2.0 minutes), or days without angina (median 100% in both) were shown. All visual analogue scores were higher with epanolol (subjective energy 58.3 +/- 1.7 vs 54.3 +/- 1.5, well-being 61.8 +/- 1.8 vs 58.6 +/- 1.5 and warmth of extremities 68.4 +/- 3.6 vs 62.0 +/- 3.1). Although these differences did not attain statistical significance, 11 patients expressed a preference for epanolol and only 6 for atenolol. We conclude that, in this study, epanolol is as effective as atenolol as an antianginal agent for chronic stable angina. It improved the side effect profile in some but not all patients.

Adrenergic beta-Agonists↗
PubMed1989

[MRT of the knee joint cartilage using 3D volume imaging in combination with a fast image processor].

From the knees of healthy persons and cadavers 2D-spin-echo-sequences were compared to 3D-gradient-echo-sequences (FLASH, FISP). We were able to show that 3D-volume-imaging, combined with image processing on a fast computer, is superior to 2D-spin-echo-imaging. The advantages are: slice thickness lower than 1 mm, secondary reconstruction in any desired plane, good contrast between intraarticular fluid, the meniscus and the hyaline cartilage. For the practical application of 3D-volume-imaging resulting in multiple images the secondary reconstruction and post processing of the large numbers of images with an image processing computer is necessary.

Cartilage, Articular↗
PubMed1989

Ambulatory electrocardiography: an open-access service for general practitioners.

Access to ambulatory electrocardiography would enable general practitioners to investigate certain patients with cardiac symptoms without the need for hospital referral. An analysis has been made of the results in 200 consecutive patients referred to a pilot open-access service based at three health centres. Twenty-two GPs used the service, although there was a wide range (1-48) in the number of patients each referred. In 72 patients aged under 50 years, abnormalities other than extrasystoles were detected in only six; major significant arrhythmias were found in three patients, although treatment was required in only one case. Arrhythmias were frequent in patients aged over 50 years but were usually minor; 16/128 (13 per cent) showed major significant arrhythmias. Pacemakers were implanted in two patients. Palpitation and/or dizziness were reported during the period of monitoring by 39 per cent of patients (and were equally common in both age groups), but in neither age group did these symptoms correlate with the occurrence of a significant arrhythmia. In primary care, palpitation and dizziness are rarely due to significant arrhythmias. To increase the cost-effectiveness of the service, ambulatory monitoring could be restricted to patients over 50 years of age, except when there is other evidence of heart disease.

Adolescent↗
PubMed1988

Prosomes, small cytoplasmic RNP particles, contain glycoproteins.

Prosomes, ubiquitous small ribonucleoprotein complexes, were isolated from the cytoplasm of erythropoietic mouse cells induced by Friend leucemia virus. We present evidence that some of the prosomal proteins are glycosylated. Specific reactions with the biotinylated lectins concanavalin agglutinin (Con A), Solanum tuberosum agglutinin (STA) and Limulus polyphemus agglutinin (LPA) indicate that the carbohydrate moieties contain N-acetylneuraminic acid, N-acetylglucosamine and mannosyl- or glucosyl-residues. Glycosylation of prosomal proteins could explain the resistance of prosomes to proteinase K digestion.

Animals↗
PubMed1988

Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1).

Control of expression of the Saccharomyces cerevisiae CTT1 (catalase T) gene by the HAP1 (CYP1) gene, a mediator of heme control of mitochondrial cytochromes, was studied. Expression of a CTT1-lacZ fusion in a hap1 mutant showed that the CTT1 promoter is under HAP1 control. As demonstrated by a gel retardation assay, the HAP1 protein binds to a heme control region of the CTT1 gene. This binding in vitro is stimulated by hemin. The HAP1-binding sequence was localized by using DNA fragments spanning different regions, by DNase I footprinting and by methylation interference of DNA-protein binding. The binding site was compared to the HAP1-binding sequences previously characterized in detail (UAS1CYC1, UASCYC7). There is strikingly little similarity between the three sequences, which have only four of those 23 bp in common which are protected from DNase I digestion. However, the pattern of major and minor groove contacts in the complex is quite similar in all three cases. The results obtained show that there is true co-ordinate control of expression of mitochondrial cytochromes and at least some extra-mitochondrial hemoproteins. Heme acts as a metabolic signal in this coordination, which is mediated by the HAP1 protein.

Base Sequence↗