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Biomedical subjects

G Abraham

Publications and source records attributed to G Abraham.

At least 145 records · Page 8Linked to original sources
PubMed1982

Thyrotoxicosis in Ethiopian patients--a prospective study.

Forty-six cases of thyrotoxicosis, 8 males and 38 females, were seen over a period of two years from March 1979. There were 19 cases of toxic multinodular goitre, 22 of Graves' disease, four of toxic adenoma and one of subacute thyroiditis. The median age of those with toxic multinodular goitre was 49 and of those with Graves' disease was 30 years. The five most common symptoms were sweating/heat intolerance (82.6%), weight loss (82.6%), palpitations (82.6%), nervousness and irritability (73.9%) and insomnia (69.6%). Six of 24 non-pregnant females of child-bearing age had menstrual disturbances. The five most common signs were goitre (97.8%), tachycardia (100.0%), sweating (84.7%), and fine finger and tongue tremors (89.1%). Among the 22 cases of Graves' disease, 13 (59.1%) had exophthalmos and 4 (18.2%) had pretibial myxoedema. Treatment with propylthiouracil or carbimazole was effective in controlling the thyrotoxicosis. Granulocytopenia developed in two cases and skin allergy in one other, necessitating use of alternative treatment. Seven cases had subtotal thyroidectomy after preparation with antithyroid drugs or propranolol and Lugol's iodine.

Adolescent↗
PubMed1982

[The couple in our culture: psychopathologic implications].

First, we should think about the meaning of the couple and then about its make-up and structure. Cultural influences on the couple will thereby be clearer. This pertains to the so-called normal couples, as well as to any pathology concerning relationships and communication.

Adult↗
PubMed1981

Diagnostic value of the Widal test.

The usefulness of a single Widal test to diagnose typhoid fever in Ethiopia was investigated in three study groups both retrospectively and prospectively. These were blood culture proven typhoid and non-typhoid patients and healthy individuals. Salmonella typhi H and O titres greater than or equal to 1:160 occurred in respectively 82% and 58% of typhoid fever patients; only 4% of healthy individuals and 8% of non-typhoid patients had Widal titres greater than or equal to 1:80. In typhoid fever, the H titre is elevated earlier and more frequently than the O titre. Antibody rise is maximal during the second week of illness. Antibiotic treatment did not affect the rise of antibody titre in typhoid fever. A single Widal test in an unvaccinated Ethiopian patient showing H and/or O titres greater than or equal to 1:160 and typhoid-like symptoms is strongly suggestive of typhoid fever. It also appears that H titre is more useful than O titre. More false positives are found than false negatives. Under these circumstances, the clinical picture is the decisive factor in making a diagnosis of typhoid.

Agglutination Tests↗
PubMed1980

Covalent labelling of specific membrane carbohydrate residues with fluorescent probes.

Two new fluorescent labelling techniques are described: one specific for sialic acid residues and a second specific for galactose and some of its derivatives. Using either technique it is possible to label covalently the desired carbohydrate residue with any one of a large variety of fluorescent probes. When the sialic acid labelling procedure is applied to human erythrocyte membranes, only the glycophorin species are labelled. However, when the galactose-directed labelling scheme is applied, fluorescence is also observed on membrane lipid components and on band 3. For each technique, the fluorescent labelling pattern is shown to reflect the distribution of the respective sugar component on the erythrocyte membrane. Thus, these techniques should provide both selectivity and versatility in the fluorescent labelling of specific carbohydrate residues in highly heterogeneous biological systems.

Electrophoresis, Polyacrylamide Gel↗
PubMed1978

Secondary amenorrhea in obesity: etiologic role of weight-related androgen excess.

A massively obese, amenorrheic young woman had elevated levels of plasma androgens which could be reduced either acutely by dexamethasone administration or chronically by weight loss. Normalization of plasma androgen levels in both instances led to resumption of ovulation, suggesting that weight-related hyperandrogenism is a cause of amenorrhea in obesity.

Adult↗
PubMed1977

Influenza virus messenger RNAs are incomplete transcripts of the genome RNAs.

The results of ribonuclease T1 oligonucleotide fingerprint analyses indicate that influenza virus messenger RNAs are incomplete transcripts of the corresponding genome RNAs and that in this respect they differ from the unpolyadenylated virus specific complementary RNAs obtained from infected cells. From the position of the untranscribed oligonucleotide in the virus RNA sequence and the ability or inability of the different transcripts to protect the 5' terminal nucleotide of virus RNAs against nuclease S1 digestion, it is concluded that whereas the unpolyadenylated cRNAs are complete transcripts, the polyadenylated messenger RNAs lack sequences complementary to the 5' end of the genome molecules.

Animals↗
PubMed1977

Vesicular stomatitis virus: mode of transcription.

Recent studies on the mechanism by which the virion-associated RNA polymerase of vesicular stomatitis virus transcribes RNA have revealed several new biological features of general interest. The mode of synthesis of the 5'-terminal cap structure of the mRNAs, the sequential transcription of the genes and the presence of a transcribed "leader" RNA segment are properties which are either not shown by other viruses, or have not yet been described. These features are probably inter-related with the primary transcription process, which itself may be a useful model for future studies on mRNA biosynthesis in eukaryotic systems.

DNA-Directed RNA Polymerases↗
PubMed1977

Characterization of vesicular stomatitis virus mRNA species synthesized in vitro.

The smallest size class of mRNA (12S) synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus contains two mRNA species of similar molecular weight that code for the viral M and NS proteins. The resolution of these mRNA species was achieved by converting them to duplexes by annealing with the genome RNA, followed by RNase T2 treatment and separation in a polyacrylamide gel. Using this separation technique, the mRNA's were identified by comparing the relative resistance of their syntheses to UV irradiation of the virus. The molecular weights of these two mRNA species calculated as duplex RNAs were smaller than expected. The possible reasons for this discrepancy are discussed.

Cell-Free System↗
PubMed1977

Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG.

Although IgG rheumatoid factor may play a central role in the pathogenesis of rheumatoid arthritis, previously there have been no precise methods for its specific measurement in serum and synovial fluid. This paper describes a solid phase radioimmunoassay for the independent quantification of IgM and IgG rheumatoid factor reacting with the Fc fragment of human IgG. As measured by this assay, serum IgG rheumatoid factor levels differed significantly between patients with seropositive and seronegative rheumatoid arthritis and normal control subjects. In addition, several sera and joint fluids from patients with seropositive rheumatoid arthritis, even without vasculitis, were shown by gel chromatography to have acid-dissociable complexes of IgG rheumatoid factor suggestive of IgG-IgG dimer or trimer formation.

Alkylation↗