Search PubMed⌕ Search

Biomedical subjects

G A Tavartkiladze

Publications and source records attributed to G A Tavartkiladze.

At least 19 recordsLinked to original sources

[Experimental and clinical audiology: current status and trends of development].

The article presents results of experimental and clinical trials obtained in the National Research Center for Audiology and Hearing Rehabilitation and other leading research centers which studied mechanisms of primary auditory perception including cellular and membrane levels; nature of hair cell motor and electromotility, otoacoustic emission generation, effects of efferent system; nature and mechanisms underlying hereditary hypoacusis and deafness; regeneration of hair cells. Rehabilitation of deaf patients by means of cochlear implantation and optimization of universal audiological screening programs are described.

Audiology↗

Hand-held device for OAE-based screening.

In this paper, the main concepts realized in a handheld OAE device are described. These concepts are: weighted averaging, non-linear MLS technique, acoustic probe with flat response, electronic passport of the probe, communication with clinical databases, special language for device control.

Acoustic Stimulation↗

Effect of stimulation parameters on electrically evoked auditory brainstem responses.

It is evident that the conventional technique for cochlear implant adjustment is not suitable for children in their first years of life. In order to find a solution to this problem, the possibility of electrically evoked auditory brainstem response (EABR) recording was investigated. EABRs were recorded in 9 patients with the Nucleus multichannel cochlear implant. The main problems that have to be solved during EABR recording in cochlear implantees are: i) EABR distortion due to the stimulus artefact: and ii) difference in the stimulus presentation rate during EABR registration (low pulse rate) and conventional psychophysical threshold estimation (high pulse rate) in cochlear implant patients. The influence of stimulus artefact on the recording results was minimized by setting the implant to the widest amplifier frequency band and by zeroing the initial segment containing the stimulus artefact with subsequent zero-phase digital filtering. The dependence of the EABR amplitude and latency on the stimulus intensity, width, electrode location and interstimulus interval was investigated. It was concluded that despite the difference revealed between absolute values of EABR thresholds and psychophysical threshold levels, it is possible to calculate implant adjustment parameters based on the EABR data with the proper correction applied.

Acoustic Stimulation↗

Cochlear outer hair cell bending in an external electric field.

We have used a high-resolution motion analysis system to reinvestigate shape changes in isolated guinea pig cochlear outer hair cells (OHCs) evoked by low-frequency (2-3 Hz) external electric stimulation. This phenomenon of electromotility is presumed to result from voltage-dependent structural changes in the lateral plasma membrane of the OHC. In addition to well-known longitudinal movements, OHCs were found to display bending movements when the alternating external electric field gradients were oriented perpendicular to the cylindrical cell body. The peak-to-peak amplitude of the bending movement was found to be as large as 0.7 microm. The specific sulfhydryl reagents, p-chloromercuriphenylsulfonic acid and p-hydroxymercuriphenylsulfonic acid, that suppress electrically evoked longitudinal OHCs movements, also inhibit the bending movements, indicating that these two movements share the same underlying mechanism. The OHC bending is likely to result from an electrical charge separation that produces depolarization of the lateral plasma membrane on one side of the cell and hyperpolarization on the other side. In the cochlea, OHC bending could produce radial distortions in the sensory epithelium and influence the micromechanics of the organ of Corti.

Animals↗

Transient evoked otoacoustic emission with unexpectedly short latency.

Two alternative approaches for studying short-latency click-evoked otoacoustic emission (OAE) in normal-hearing subjects were employed. Growth of a click-evoked "ear canal response" with stimulus increase became progressively more non-linear and saturated when the latency of the analyzed segment of response increased. This relation between latency and shape of the response input/output function was observed even after linear component cancellation, indicating that it could be an intrinsic property of OAE. Hence, the existence of an essentially linear short-latency OAE component which is probably eliminated by commonly used artifact cancellation technique is suggested. Taking into account the fact that transient evoked otoacoustic emission (TEOAE) may be completely suppressed by simultaneously presented noise, a "true" artifact cancellation was performed by subtracting the ear canal response in the presence of a masker from the conventional click-evoked OAE recording. An additional TEOAE component with a latency of 2.5-5 ms was found. Its growth with stimulus intensity was indeed more linear than that of later components. However, latency and frequency of this TEOAE component, being specific for each subject, can hardly be explained by both a commonly assumed latency-frequency relationship of TEOAE and a generally used estimation of TEOAE latency as the sum of the forward and backward traveling wave propagation times.

Acoustic Stimulation↗

Ipsilateral suppression of transient evoked otoacoustic emission: role of the medial olivocochlear system.

Contralateral sound stimulation produces suppression of transient evoked otoacoustic emission (TEOAE), which is attributed to a reflex activation of the medial olivocochlear system. More pronounced suppression of TEOAE produced by ipsilateral masking could involve efferent-mediated effects along with effects of cochlear origin. However, this has not been investigated so far. Therefore, changes of click-evoked OAE under ipsi- and contralateral forward masking by clicks and noise-bursts were investigated in an extremely long-lasting experiment in one normal-hearing volunteer. The reduction of TEOAE under ipsilateral click-to-click forward masking was maximal during the first milliseconds after masker delivery implying that predominant role of the cochlear processes in TEOAE ipsilateral suppression. Ipsilateral forward masking by noise burst revealed additional TEOAE suppression with longer latency. Its time course was similar to that of the contralateral masking effect. The latter data suggest the involvement of the medial olivocochlear system in TEOAE ipsilateral suppression.

Acoustic Stimulation↗

Badania wywolanej otoemisji akustycznej. [Tuning properties of the evoked otoacoustic emission].

The authors present the investigation of evoked otoacoustic emission (EOAE) as a part of the studies of the inner ear function. The EOAE was obtained in 10 normal hearing subjects during the routine recording conditions. In the investigation was used the adjustment of masking tone to obtain a reduction in the (EOAE) most stable component amplitude and suppression of the (EOAE) frequency components (FFT--derived).

Audiometry, Pure-Tone↗

A cyclic-AMP-gated conductance in cochlear hair cells.

The patch clamp technique was used to record cAMP-dependent currents of the guinea pig cochlear hair cell plasma membrane. Data obtained indicate that the channels passing this current are moderately selective for monovalent cations and are effectively blocked by L-cis-diltiazem and reversibly blocked by 1 mM Mg2+ or Ca2+. The single-channel unit conductance estimated in the absence of divalent cations is about 16 pS. The results demonstrate that cyclic nucleotide-dependent channels of cochlear hair cells are virtually identical to the photoreceptor and olfactory ones.

Animals↗

Effects of glycerol on cochlear frequency selectivity in patients with Menière's disease.

During transtympanic electrocochleography, action potential (AP) tuning curves were derived as a measure of cochlear frequency selectivity. In 18 out of 21 patients with symptoms of Menière's disease, a poor frequency selectivity was observed. In 12 subjects the effects on frequency selectivity of oral glycerol were measured, and in 5 patients AP tuning curves indicated an improvement in sharpness of tuning by a factor of 1.5-2.5. These changes started approximately 30 min after oral glycerol administration. In 2 subjects there was a deterioration in AP tuning. In 5 patients no significant changes were observed.

Action Potentials↗

[Frequency selectivity of the normal guinea pig cochlea and in experimental hearing loss].

The frequency selectivity of the cochlea was measured in pigmented guinea pigs with implanted electrodes using simultaneous masking paradigm. In animals with experimentally induced deafness, AP tuning curves are broadened by a factor of 2-3 as compared with normal animals. The deterioration of frequency selectivity is thought to correlate with extent of the outer hair cells loss. The deterioration of threshold and tuning properties of the cochlea seem to be induced by the loss of the functional integrity of the outer hair cells.

Acoustic Stimulation↗

Electrophysiological measures of cochlear function in guinea pigs with long-term endolymphatic hydrops.

Experimental endolymphatic hydrops was induced in guinea pigs by obliteration of the endolymphatic sac and duct. From 3 to 24 months after this operation, cochlear action potential (AP) audiograms and AP tuning curves were measured. The purpose of this study was to establish parallels, if any, between this supposed animal model of Menière's disease and the auditory symptoms of the disease in man. In some animals, low and middle frequency AP threshold elevations were observed whilst higher frequency regions maintained normal sensitivity. Other animals developed flat or very gradually sloping AP audiograms. These patterns are qualitatively similar to those found clinically in Menière's disease. AP tuning curves measured in frequency regions of threshold elevation indicated a deterioration of cochlear frequency selectivity; psychophysical and electrocochleographic studies demonstrate related changes in Menière's patients. One animal exhibited modifications in AP thresholds and tuning as a result of glycerol administration. These observations improve our confidence in the validity of this animal model for further studies of the pathophysiology of Menière's disease.

Action Potentials↗

Ipsilateral suppression effects on transient evoked otoacoustic emission.

The spectral properties of click- and tone-evoked otoacoustic emission (OAE) under ipsilateral simultaneous tonal masking conditions as well as the changes of click-evoked OAE under ipsilateral forward masking by clicks were studied in normal-hearing subjects. It was found that (i) transiently evoked OAE (TEOAE) spectra consist of a number of peaks individual to the subject and distributed along a sufficiently wide cochlear region; (ii) each spectral peak is excited only when the stimulus energy was concentrated within the frequency range covering the frequency of the particular peak; (iii) different TEOAE spectral peaks can be masked independently under simultaneous tonal masking conditions; (iv) the TEOAE tuning curve shape is more closely related to the spectrum of TEOAE but not to that of the stimulus; (v) forward masking of TEOAE generation is most pronounced during the first few milliseconds after masker click onset and become significantly smaller with the longer latency. It is suggested that: (i) TEOAE is generated by a number of local generators individually distributed along the cochlear partition within a sufficiently wide region and characterized by different power; (ii) possible efferent effects in the ipsilateral TEOAE suppression are negligible as compared to the TEOAE reduction of exclusively cochlear origin.

Acoustic Stimulation↗