No secrets, no surprises: understanding the NBRC (National Board for Respiratory Care) credentialing exams.
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Credentialing↗
Biomedical subjects
G A Smith
Publications and source records attributed to G A Smith.
Analysis of the primary signals required for activation of the mitogenic pathway in murine thymocytes from protein phosphorylation patterns.
It has been proposed that phorbol esters and the Ca2+ ionophore A23187 are effective comitogens for some species of lymphocytes because together they mimic the normal secondary signals for cell activation by mitogens that cause phosphatidylinositol 4,5-bisphosphate (PtdInsP2) breakdown (e.g., anti-TCR and anti-Thy-1 antibodies and Con A). To test whether activation of protein kinase C and an increase in [Ca2+]i account for the activation of the mitogenic pathway in murine thymocytes by the mitogens that cause PtdInsP2 breakdown, the two-dimensional phosphorylation patterns generated by the three classes of mitogens (protein kinase C activator, Ca2+ ionophore, and activator of PtdInsP2 breakdown) and by activators of cAMP-dependent kinases have been compared. From the phosphorylation patterns, by which each mitogen could be distinguished reproducibly, it was concluded that: 1) The phosphorylation patterns generated by the mitogens that activate PtdInsP2 breakdown are only slightly affected by the removal of extracellular Ca2+ under conditions that abolish the normal rise in [Ca2+]i and do not therefore depend on the activation of Ca2(+)-dependent protein kinases. In contrast, the phosphorylation pattern generated by A23187 is totally dependent on extracellular Ca2+. 2) Neither A23187 nor the mitogens that activate PtdInsP2 breakdown nor activators of cAMP-dependent kinases caused significant activation of protein kinase C assayed by phosphorylation of the diagnostic proteins 80b and 78a. Consistent with this conclusion, only the phorbol esters or oleoyl acyl glycerol caused translocation of protein kinase C activity from the cytosolic to the membrane fraction. 3) Neither A23187 nor the mitogens that cause PtdInsP2 breakdown activated cAMP-dependent kinases. Taken together the data imply that the mitogens that cause PtdInsP2 breakdown must generate an additional, independent primary mitogenic signal. It is suggested that this signal may be the activation of tyrosine kinases (e.g., p56lck) via the TCR and working hypotheses for effective combinations of primary mitogenic signals that will activate DNA synthesis are developed.
Animals↗
Transdermal iontophoresis of gonadotropin releasing hormone (LHRH) and two analogues.
Gonadotropin releasing hormone (GnRH), as well as an antagonist [Ac-D2Nal,1 D4ClPhe,2 D3Pal,3 NicLys,5 DNicLys,6 ILys,8 DAla10] GnRH.HOAc (1) and a superagonist [DTrp6, Pro9-NHEt]GnRH (2), have been electrochemically driven across excised hairless mouse skin. Determined by HPLC analysis, the delivery rate from aqueous solution into isotonic saline at 0.5 mA cm-2 was as high as 19 nM cm-2 h-1 for 2. Because of its insolubility in water, analogue 1 could only be delivered from an acidic donor solution. Analogue 2 was also delivered in pulsatile fashion using current on/off cycles. For all three peptides, passive transport was negligible and stability is evident when in contact with the stratum corneum. Slow metabolism occurs when GnRH contacts the dermal side of hairless mouse skin.
Animals↗
The information-loss model: a mathematical theory of age-related cognitive slowing.
A model of cognitive slowing is proposed with the following assumption: Information is lost during processing, processing occurs in discrete steps with step duration inversely related to the amount of information currently available, and the effect of aging is to increase the proportion of information lost per step. This model correctly predicts a positively accelerated relation between latencies of older and younger adults and provides a unified account of the effects of task complexity, practice, speed-accuracy tradeoffs, and fluctuations in individual performance. Strong support for the thesis that cognitive slowing is global, and not localized in specific age-sensitive components, is provided by the fact that the model accurately predicts the latencies of older adults on the basis of those of younger adults, without regard to the nature of the task, across a latency range of nearly 2 orders of magnitude.
Adult↗
Synthesis of fluorodeoxy analogues of myo-inositol.
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Fluorine↗
Assessing sincerity of effort in maximal grip strength tests.
Injuries to the upper extremities sometimes result in a loss of work capacity. One of the methods for measuring this loss of work capacity is the grip test. Such measurements may be compromised by an insincere effort (faking) by the subject. Smith et al. (Am J Phys Med Rehabil 1989;68:73-80) developed a protocol to identify sincere and faking efforts with the use of a modified dynamometer. The present investigation was designed to evaluate the applicability of the previously developed protocol to subjects with hand injuries. The investigators also compared the force-time characteristics for contractions at different percentages of maximum (100, 75, 50, and 25%). Sixty subjects (30 male and 30 female) were tested during two sessions while performing both faking and sincere efforts. The five discriminators previously developed were found to be good detectors for both noninjured and injured hands and provided the basis for cutoff values for the five discriminators. Single discriminator prediction gave at best an 85% faking detection rate (by using D3) for men and a 79.2% detection rate (by using D5) for women. Better results were obtained, when a multiple variable prediction was used. It can be concluded that the protocol developed by Smith et al. for detection of an insincere effort can be used successfully with persons who have sustained upper extremity injuries. Furthermore, sincere and faking grip strength "maxima" can be correctly identified across a wide range of applied force levels.
Adult↗
The location and nucleotide sequence of the thymidine kinase gene of bovine herpesvirus type 1.2.
On the basis of their restriction endonuclease digestion patterns, four Australian bovine herpesvirus type 1 (BHV-1) isolates were classified as belonging to the BHV-1.2a subtypes. The thymidine kinase (TK) genes of all four BHV-1.2a isolates were located on a 3.5 kb SalI restriction fragment. This is in contrast to North American and European BHV-1.1 isolates whose TK genes are contained on a 2.6 to 2.8 kb SalI fragment. The restriction fragments containing the TK genes were cloned into phagemid vectors and their sequences determined using the dideoxynucleotide chain termination method. The BHV-1.2a isolates possessed identical TK gene sequences, which differed from previously published TK sequences for the LA and 6660 BHV-1.1 strains. In addition to five single base alterations, there were six separate base insertions which resulted in two major frameshifts which spanned an area of 72 amino acids or 20% of the expressed TK gene product. The predicted amino acid sequence exhibited a higher degree of similarity to other herpesvirus TKs, suggesting that previously published TK gene sequences may have been incorrect. The present nucleotide sequence and corresponding amino acid composition reinforces previous observations concerning regions of herpesvirus TK amino acid conservation and should assist in future studies into the evolution and functional domains of herpesvirus TKs.
Amino Acid Sequence↗
Use of 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5FBAPTA) in the measurement of free intracellular calcium in the brain by 19F-nuclear magnetic resonance spectroscopy.
We have applied the 19F-nuclear magnetic resonance (NMR) calcium indicator 1,2-bis(2-amino-5-fluoro-phenoxy)ethane-N,N,N',N'-tetraacetic acid (5FBAPTA) to the measurement of the free intracellular calcium concentration [( Ca2+]i) in superfused brain slices. A mean +/- SD control value of 380 +/- 71 nM (n = 18) was obtained at 37 degrees C using 2.4 mM extracellular Ca2+. Subcellular fractionation studies using [3H]5FBAPTA showed that after loading of its tetraacetoxymethyl ester, approximately 55% was de-esterified, with the other 45% remaining as the tetraester bound to membranes. Of the de-esterified 5FBAPTA, greater than 90% was in the cytosolic fractions, with less than 1% in the mitochondria or microsomes. The NMR-visible de-esterified 5FBAPTA slowly disappeared from the tissue with a t1/2 of 4 h. A time course after loading confirmed that the calculated [Ca2+]i was constant over a 5-h period, although the scatter of individual results was +/- 20%. The [Ca2+]i was increased by a high extracellular K+ concentration ([K+]e), by a low extracellular concentration of Na+, and by the calcium ionophore A23187. On recovery from high [K+]e, the [Ca2+]i "overshot" to values lower than the original control value. The [Ca2+]i was surpisingly resistant to changes in extracellular Ca2+ concentration.
Animals↗
Activities of 3'-azido-3'-deoxythymidine nucleotide dimers in primary lymphocytes infected with human immunodeficiency virus type 1.
The relative antiviral potencies of five nucleotide heterodimers of 3'-azido-3'-deoxythymidine (AZT), 3'-azido-3'-deoxythymidilyl-(5',5')-2'-3'-dideoxy-5'-adenylic acid (AZT-P-ddA), 3'-azido-3'-deoxythymidilyl-(5',5')-2',3'-dideoxy-5'-inosinic acid (AZT-P-ddI), and the corresponding 2-cyanoethyl congeners AZT-P(CyE)-ddA and AZT-P(CyE)-ddI, were determined in primary human peripheral blood mononuclear cells infected with human immunodeficiency virus type 1. The homodimer 3'-azido-3'-deoxythymidilyl-(5',5')-3'-azido-3'-deoxythymidilic acid (AZT-P-AZT) was also included for comparison. The potencies of the compounds were AZT-P-ddA greater than or equal to AZT-P-ddI greater than AZT-P(CyE)-ddA greater than or equal to AZT-P(CyE)-ddI greater than or equal to AZT greater than AZT-P-AZT. Whereas AZT-P-ddA and AZT-P-ddI had in vitro therapeutic indices greater than that of AZT, the homodimer of AZT had a low therapeutic index. AZT-P-ddI exhibited the lowest toxicity in peripheral blood mononuclear, Vero, or CEM cells. Combination studies between AZT and 2',3'-dideoxyinosine (ddI) at nontoxic concentrations indicated a synergistic interaction at a drug ratio of 1:100. At higher ratios (1:500 and 1:1,000), the interactions were synergistic only at concentrations that produced up to 75% virus inhibition. At higher levels of antiviral effects, this combination was antagonistic, as determined by the multiple drug effect analysis method. AZT-P-ddI was about 10-fold less toxic than AZT to human granulocyte-macrophage progenitor cells. However, no significant difference was apparent when the compounds were evaluated against cells of the erythroid lineage. The greater antiviral activity and lower toxicity of this compound could not be attributed to the extracellular decomposition of the dimer in media at physiological temperature and pH. However, in acidic solutions, AZT-P-ddI decomposed in a pH-dependent manner. Advanced preclinical studies with this heterodimer of two clinically effective antiretroviral agents should be considered.
Cell Survival↗
Biomechanics of crosscountry skiing.
The mechanics of crosscountry skiing involve a complex interaction between the kinematic characteristics of the movement patterns and the kinetic relationships driving the motion. Crosscountry skiing techniques have been the subject of some biomechanical research, primarily involving the traditional diagonal stride technique. In the 1980s, skiers have developed several new approaches to moving across snow. These skating techniques rely on the generation of force from ski placements at an angle to the forward direction creating propulsive force while the ski slides forward. Biomechanical analysis of the crosscountry techniques has developed from rather simple 2-dimensional kinematic descriptions of diagonal stride to complex measurement of skating forces and 3-dimensional motion. The combined determination of forces and resultant motion has perhaps the most promise for practical application in assessments of equipment and technique. The relationship of the kinematic characteristics of skiing to the forces involved have received preliminary study but substantial gains in understanding will be necessary before approaches to optimising a skier's technique are plausible.
Biomechanical Phenomena↗
Processing speed and mental retardation: deadline procedures indicate fixed and adjustable limitations.
Brewer and Smith (1984) showed that control mechanisms mediating speed-accuracy regulation contribute to retarded-nonretarded differences in processing speed, with poorly controlled trial-to-trial RT adjustments underlying the greater RT variability of retarded individuals. In Experiment 1, response deadlines controlled processing time, thus minimizing the influence of such control mechanisms. The obtained speed-accuracy relations showed that retarded subjects were unable to match nonretarded subjects' accuracy when responding as rapidly, thus indicating structural limitations on processing speed. The results of Experiment 2 showed, however, that significant adjustments to retarded subjects' processing speed--exceeding those produced by practice--are achievable. Extended training at a short deadline led to tighter control of RT adjustments, with substantial improvements in mean RT when subjects transferred to a self-paced RT task.
Adolescent↗
Identification and immunochemical characterization of the human erythrocyte membrane glycoproteins that carry the Xga antigen.
When the membrane components of Xg(a+) erythrocytes were separated by electrophoresis and immunoblotted with an anti-Xga of human origin, two diffuse bands of approximate Mr 22,000-25,000 and 26,500-29,000 were stained. These reactive components were not evident in membranes from proteinase-treated Xg(a+) erythrocytes. Neuraminidase treatment of erythrocytes before immunoblotting resulted in one diffuse Xga-reactive band, the leading edge of which had a slightly increased mobility corresponding to a decrease in Mr of approx. 1500. These results suggest that the membrane components that carry Xga are sialoglycoproteins. A genetic relationship exists between Xga and the antigen recognized by the monoclonal antibody 12E7. An immunochemical comparison of the structures that carry the Xga and 12E7 antigens demonstrated that they differ in Mr and in the way in which they are modified by neuraminidase. This is in accord with evidence that Xga and 12E7 are the products of two separate structural loci.
Antigens, Surface↗
The immediate or delayed replacement of teeth by permucosal intra-osseous implants: the Tübingen implant system. Part 2: Surgical and restorative techniques.
The previous paper emphasised the importance of case selection in determining success in the use of the Tübingen implant system. Adherence to strict clinical techniques has an equally important influence on implant success and failure. This paper describes the surgical and restorative techniques involved in the use of the Tübingen implant and comments on the authors' initial experiences with this system.
Aluminum Oxide↗
The immediate or delayed replacement of teeth by permucosal intra-osseous implants: the Tübingen implant system. Part 1. Implant design, rationale for use and pre-operative assessment.
No single implant system is applicable to all clinical problems which may be treated with implants. Clinicians who wish to develop expertise in the field of dental implantology should decide which clinical situations they wish to treat with implants and then receive an appropriate level of instruction in the use of systems which have been shown, in refereed literature, to be successful in the treatment of such problems. The Tübingen implant system has been in clinical use for more than 13 years, following preliminary basic research and animal studies, and is supported by an extensive literature. It is applicable primarily to the immediate or delayed replacement of individual teeth which are subsequently to be restored with a post crown. It may also be used in other situations, such as in combination with bridge restorations. This is the first of two papers which describe in detail the range of applications, patient selection and assessment, and the surgical and restorative techniques involved in its use.
Adult↗
Assessing sincerity of effort in maximal grip strength tests.
The measurement of maximum hand grip strength in ergonomic or clinical settings has been a useful means of assessing physical characteristics, progress in rehabilitation and degree of disability in upper extremity injuries. The validity of the peak forces observed in such measurements is compromised by the requirement of subject cooperation in giving a maximum effort. Thus, an easily administered analysis of subject sincerity would improve this basic strength-testing tool. Several variables were developed for the discrimination of faking (submaximal) from sincere (maximal) grip contractions. A microcomputer-based grip force data collection system was assembled in which analog output from a Jamar hand dynamometer was sampled at 200 Hz and digitally analyzed. A total of 43 normal subjects (20 male, 23 female) were tested under sincere and faking conditions (three trials of each condition for each hand). The force-time curves of each trial were analyzed for peak and average forces and force variability. From these basic parameters five discriminator variables were developed. The frequency distribution of the sincere values for each of these variables was used to determine a criterion value for discrimination of sincere from faking trials. The five discriminators correctly detected 95.0, 92.5, 100, 100 and 97.5% of the male faking trials. Female faking was less successfully detected: 59.7, 52.2, 78.3, 71.7 and 87.0% correct detection resulted for the variables (with a 95% confidence level of correctly identifying sincerity). Multiple variable predictions improved the female faking detection up to 93.5% with little apparent decrement in sensitivity to sincerity identification.(ABSTRACT TRUNCATED AT 250 WORDS)
Adolescent↗
Partial characterisation of the human erythrocyte antigen Pta.
Ghosts were prepared from erythrocytes positive for the rare blood group antigen Pta. Immunoblotting of the solubilised ghosts with anti-Pta located the antigen on a band with an Mr of 31,600, about 1,100 higher than that of sialoglycoprotein gamma. Binding to the same band was also observed when cytoskeleton preparations from Pt(a+) erythrocytes were immunoblotted with the antibody. Haemagglutination and immunoblotting experiments were consistent in demonstrating that the Pta antigen is not inactivated by treatment of intact erythrocytes with neuraminidase or trypsin but is destroyed by treatment with alpha-chymotrypsin, papain or pronase. The data indicate that the Pta antigen is carried on a 'new' erythrocyte membrane protein.
Blood Group Antigens↗
The influence of breed and sex on the incidence of mortalities and skin tears in broiler carcasses.
The effects of nutrition, breed and sex on the incidence of mortalities and of skin tears on broiler carcasses were studied. Both sexes of 2 breeds, Hubbard and Ross, were given 4 dietary treatments consisting of a normal level of vitamin and mineral premix plus 300 ppm furazolidone, twice normal premix inclusion level plus 300 ppm furazolidone, a normal level of vitamin and mineral premix, and twice normal mineral premix inclusion level. A coccidiostat containing the active ingredient halofuginone was included at 6 ppm, twice the recommended dosage. Starter, grower and finisher diets were fed respectively up to Day 20, Day 30 and Day 48. Group growth performances, mortalities and the incidence of torn skin were monitored on both the live birds and carcasses. The effect of the different nutritional treatments on skin tears was not significant (P = 0.9533), as was the breed effect (P = 0.0547). However, the effect of sex was significant (P = 0.0044), the incidence in hens being higher. Mortalities among the Hubbard were significantly greater than among the Ross (P = 0.0001). Hens showed a slightly higher mortality rate than roosters, tending towards significance at the 5% level (P = 0.0554).
Animal Nutritional Physiological Phenomena↗
Detection and quantitation of platelet-associated IgG in thrombocytopenia: a comparison of immunofluorescence and immunoenzyme techniques.
A platelet suspension immunofluorescence test (PIFT) was compared with an enzyme-linked immunosorbent assay (ELISA) for measurement of platelet-associated IgG (PAIgG) in 108 patients with thrombocytopenia. The ELISA appeared to have greater sensitivity than the PIFT in detecting PAIgG in patients with idiopathic thrombocytopenic purpura, and in other groups of patients studied, and in many cases results were discrepant between the two assays. Possible explanations are given for the low sensitivity of the PIFT, and for the discrepant results.
Blood Platelets↗