[Effect of long-term irrigation on the soil microflora of southern Ukraine].
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Biomedical subjects
Publications and source records attributed to G A Iutinskaia.
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Homeostatic potentialities of microbial communities in soils contaminated with heavy metals have been studied. To estimate the effect of contaminants on the soil microflora it was proposed to use the indices characterizing homeostasis of microbial community: microorganism survivability in contaminated soil and the period of restoration of their number, sensitivity to certain contaminants. In the model experiments on dark-grey podzolized soil it was shown that contamination with heavy metals (Cu2+, Cd2+, Sr2+, Pb2+, Hg2+) in the doses of 2 and 4 maximum permissible concentrations provokes a short-term inhibition of microorganisms development after which their number restores. Introduction of the mixture of metals evokes more intensive inhibition and extends the period of microorganism number restoration as compared with the effect of certain metals in the same doses.
A method has been developed to determine the molecular-weight distribution of biologically active substances: bacterial lectins and soil humus compounds. The method based on the simultaneous centrifugation of samples and molecular weight standards in the density gradient of NaCl solutions or combined gradient of NaCl and CsCl solutions permits analysing biologically active substances: lectins, proteins, polysaccharides, protein-polysaccharide complexes, humus compounds in the interval of molecular weight of 13.7 kappa [symbol: see text] a to 2000 kappa [symbol: see text] a. The use of this method in the soil researches makes it possible to study the dynamics of change of molecular parameters of the soil organic matter depending on agrotechnical methods as well as to determine transformation regularities of microbial polysaccharides.
A historical essay of creative path of collective of the Department of General and Soil Microbiology, founded in 1928, is presented. Two research schools have been formed for the past years: ecophysiology of soil microflora and geochemical activity of microorganisms. L. I. Rubenchik stood at the sources of these schools. A problem of soil biology and microorganisms interrelations with higher plants occupies a considerable place in the Department research. Separate groups, microorganisms and microbe cenoses are studied as a whole as the complex self-regulated system. The investigations of microbe corrosion of metals initiated by a necessity to solve urgent problems of Kyiv metro have been formed as the priority trend. Agents and mechanisms of biological damages of the overground, underground and underwater structures are studied and the grounds of anticorrosion measures are developed within the framework of these trends.
Systematic position of 49 cultures from Ukrainian Collection of Microorganisms (UCM) has been revised using the computer program StmID. Forty five cultures retained their species status but 4 cultures received the new species. Authors make a critical estimate of the program work and propose some recommendations for users.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.