Search PubMed⌕ Search

Biomedical subjects

G A Gole

Publications and source records attributed to G A Gole.

35 records · Page 2Linked to original sources
PubMed1987

Laminin-like immunoreactivity in induced new vessels in kitten and mouse eyes.

Laminin is a basement membrane glycoprotein, widely distributed in body tissues. It has been found on capillary basement membranes in both developing and adult retinal vessels. Oxygen-induced retinopathy was produced in kittens and mice by exposing them to a high oxygen environment during the newborn period. When the animals developed a proliferative retinopathy after a period of survival in room air, they were sacrificed and the eyes enucleated and embedded in paraffin wax. Cross-sections were cut and de-waxed. After preliminary digestion with pepsin, we used a fluorescein isothiocyanate (FITC)-labelled double antibody technique to identify laminin-like immunoreactivity (LLIR). LLIR was found on both intravitreal and intraretinal new vessels in the kitten and mouse, indicating that it is probably secreted by endothelial cells during the formation of pathological new vessels.

Animals↗
PubMed1987

Localization of laminin to retinal vessels of the rat and mouse using whole mounts.

Using a whole mount procedure in adult and neonatal mice and adult rats, we have developed an immunohistochemical method for the localization of laminin-like immunoreactivity (LLIR) to the retinal vessels. LLIR was localized to the vascular basement membrane, permitting a clear three-dimensional view of the retinal vasculature. Positive stain was seen in the inner limiting membrane, in retracted capillaries, encasing pericytes, and in a banding pattern on retinal arterioles. The major findings with the whole mount preparations were confirmed using paraffin-embedded material, with the additional observation of LLIR in the lens capsule. In whole mounts of retinas from neonatal mice, LLIR was present from the earliest stages of capillary growth, indicating that laminin is likely to be secreted by endothelial cells during retinal angiogenesis. LLIR within the retinal nerve fiber layer does not precede capillary ingrowth, so no evidence was found that laminin acts as a tracker signal for retinal angiogenesis.

Animals↗
PubMed1986

Combined hamartoma of the retina and RPE: an unusual cause of the dragged disc appearance.

Combined hamartoma of the retina and retinal pigment epithelium (RPE), is a rare, benign, congenital fundal lesion which contains a mixture of glial tissue, blood vessels and pigment epithelium. Such tumours are variably pigmented and may be wrongly diagnosed as malignant melanoma or retinoblastoma. We report two cases which were located in the periphery of the fundus and which produced a dragged disc appearance.

Child, Preschool↗
PubMed1985

Massive choroidal haemorrhage as a complication of krypton red laser photocoagulation for disciform degeneration.

A 78-year-old man with perifoveal subretinal neovascularization originally treated with krypton red laser was re-treated. An attempt was made to produce a "chalky white" burn at the site of the neovascular frond. Immediately after a burn, a brisk choroidal haemorrhage followed, apparently from a ruptured choroidal vessel. Bleeding occurred subretinally and into the vitreous, blinding the patient. While the krypton red laser carries advantages over the argon laser in the treatment of subretinal neovascular fronds, this case indicates that hard burns with the krypton laser carry a risk of massive subretinal haemorrhage.

Aged↗
PubMed1985

A microprocessor-driven one-operator forced-choice preferential looking apparatus.

Forced-choice preferential looking (FPL) is a behavioural technique for the estimation of visual acuity in preverbal children. We have constructed an electromechanical FPL apparatus controlled by a microprocessor requiring just one operator. Sixty-three babies aged between six weeks and eleven months were evaluated with an overall success rate of 83% in obtaining an acuity measurement. FPL is a practical method for measurement of infant visual acuity in the ophthalmic clinic setting.

Behavioral Medicine↗
PubMed1983

Angiogenic factors and their assay: activity of formyl methionyl leucyl phenylalanine, adenosine diphosphate, heparin, copper, and bovine endothelium stimulating factor.

A number of chemically unrelated substances have been compared for their neovasculogenic activity by two different in vivo tests, and for their ability to induce cultured endothelial cell migration and proliferation. Formyl methionyl leucyl phenylalanine, copper ions, heparin, adenosine diphosphate, and low-molecular-weight bovine endothelium stimulating factor were all neovasculogenic by the corneal pocket assay. By the chorioallantoic membrane assay, copper ions and formyl methionyl leucyl phenylalanine were not detectably neovasculogenic. By the same assay it has been possible to demonstrate angiogenic activity in bovine endothelium stimulating factor that is distinguishable from copper ions. This bovine factor, in contrast to the other agents, induced marked endothelial cell migration and also proliferation and may belong to a special class of agents which represents direct acting angiogenic activity.

Adenosine Diphosphate↗
PubMed1982

Oxygen induced retinopathy: the kitten model re-examined.

We have studied the kitten model of oxygen induced retinopathy by the technique of corrosion vascular casting/scanning electron microscopy. The predominant morphological features of the kitten model are preretinal and intraretinal neovascularization with a minor component of arterio-venous shunting. This is contrasted with the active phase of the blinding disease in the human neonate which is characterised predominantly by so called 'mesenchymal arterio-venous shunting'. A failure to appreciate fundamental differences in the nature of oxygen induced retinopathy between the human infant and its kitten model we believe has led to erroneous conclusions about pathogenesis in the human neonate.

Animals↗
PubMed1980

Cellular and molecular mechanisms in angiogenesis.

Low concentrations of copper sulphate, Dispirin, or Walker carcinoma extract elicit intraocular vascularization when tested by anterior chamber implants in rats. The response is markedly depressed by pre-treatment of animals with methylprednisolone acetate, suggesting that such induced vascularization is mediated by leucocytes. Since many agents inducing vascularization also induce migration of cultured endothelial cells, it raises the problem of how to isolate and study the action of possible leucocyte-derived angiogenic factors. Regardless of the identity of the natural angiogenic factor or factors, it is proposed that during blood vessel formation specialized endothelial cells migrate in response to an angiogenic signal and deposit fibronectin on which other cells can track and subsequently adhere, forming an endothelium.

Animals↗
PubMedDate not supplied

Results of treatment of superior oblique overaction by silicone tendon-expander technique.

PURPOSE: To report the results of treatment of a series of patients with superior oblique overaction using the superior oblique silicone tendon-expander technique. METHODS: A chart review of 17 patients with superior oblique overaction who had a total of 26 silicone tendon-expander procedures was conducted. RESULTS: Mean preoperative degree of superior oblique overaction was +2.7. 92% of eyes had mild (+1) or no residual overaction at last postoperative assessment (follow-up range: 6 to 59 months). Of 15 patients with preoperative A-pattern of 10 prism diopter (delta) or more, only two patients (13%) had A-pattern of 10 delta or more at last assessment. Of 13 patients with preoperative hypotropia in primary position, five patients (38%) had no vertical deviation in primary position, and seven patients (54%) had persistent, but less vertical deviation in primary position at last assessment (mean reduced from 11 delta to 4 delta). No patient manifested superior oblique palsy at their last postoperative assessment. CONCLUSIONS: We believe that the superior oblique tendon-expander technique should be strongly considered for the treatment of superior oblique overaction associated with A-pattern or hypotropia in primary position, because it has a high success rate and a low incidence of postoperative complications. Consecutive superior oblique underaction did not occur in this series.

Adult↗