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Biomedical subjects

G A Cottrell

Publications and source records attributed to G A Cottrell.

139 records · Page 8Linked to original sources
PubMed1970

A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart.

1. A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam (Mya arenaria) heart.2. Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 10(5) times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, gamma-aminobutyric acid, glutamic acid, histamine, 5-hydroxytryptamine and noradrenaline.3. Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.

Acetylcholine↗
PubMedDate not supplied

Identification and probable role of a single neurone containing the neuropeptide Helix FMRFamide.

Recently, there has been intense interest in the possibility that peptides might function as neurotransmitters. Despite much progress, there remains no clear-cut example in which the production of a chemically characterized peptide may be ascribed to individual identifiable neurones of proven physiological role. Invertebrate systems have proved to be particularly valuable for the study of identified neurones, including those producing peptides. We have now identified a neurone in ganglia of Helix that is associated with a peptide of the Phe-Met-Arg-Phe-NH2 (FMRFamide) group, and which influences tentacle contraction. This system offers for the first time the capacity to study peptidergic transmission in a system in which both the cell soma and the postsynaptic target may be readily and reproducibly identified.

Animals↗