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Biomedical subjects

Feng Wang

Publications and source records attributed to Feng Wang.

At least 217 records · Page 12Linked to original sources

[Effects of amino acids on the survival of heat-exposed Drosophila].

To investigate the effects of amino acids on the survival of heat-exposed Drosophila and to select the amino acids that can protect the Drosophila from heat exposure, Drosophila melanogaster were fed with basic culture in 25 degrees C. The male virgin Drosophilas were distinguished from female. Both male and female virgin Drosophilas were divided respectively to the trail groups and control group with randomization. The trail groups were fed with culture supplying different amino acids in 37.5 degrees C. After 10 hours, the dead insects were counted every two hours until all of them were dead. The mortality rates of heat-exposed Drosophilas that feed in the culture added with the glutamine, arginine, phenylalanine, tyrosine and glutamic acid were obviously higher than that of control. The glutamine, arginine, phenylalanine, tyrosine and glutamic acid might increase survival time of heat-exposed Drosophila, but a dose-response relationship was not observed.

Adaptation, Physiological↗

[Cloning and sequence analysis of a novel stage-specific cDNA from adult Trichinella spiralis].

OBJECTIVE: To clone a stage-specific novel cDNA from 5 day-old adult worm (AD5) of Trichinella spiralis. METHODS: The cDNA library of AD5 was screened by an AD5 stage-specific cDNA probe labeled with digoxigenin (DIG). The positive clones were sequenced and analysed. RESULTS: The positive clone contained a cDNA insert of 1,132 bp in length with a full length open reading frame (ORF) of 1,032 bp. The cDNA encoded a polypeptide of 343 amino acid residues(aa) with a molecular weight of 35.1 kDa and an isoelectric point (IP) of 4.8. InterProScan analysis showed that the 117-120 aa (SGYG) was a glycosaminoglycan attachment site, 27-86 aa was nematode cuticle collagen N-terminal domain and 153-228 aa was collagen repeat (G-x-y) domain. Signal PV2.0 analysis indicated that the region of 1-43 aa was a signal peptide. Blastn homology analysis in Genbank revealed that the cDNA had no obvious homology to any other known gene sequence. Blastn analysis revealed high homology to cuticle collagen with identities more than 40%. CONCLUSION: A novel AD5 stage-specific cDNA encoding a full length ORF was cloned and sequence analysis showed this gene encoded cuticle collagen of Trichinella spiralis.

Amino Acid Sequence↗

[The expression of Bcl-2 protein on acute myocardial ischemia/reperfusion and the significance in forensic pathology].

OBJECTIVE: To search the objective morphologic evidence for the forensic pathological diagnosis on the acute myocardial ischemia/reperfusion. METHODS: The expression of Bcl-2 protein in the early stage of acute myocardial ischemia/reperfusion(I/R) in rats were studyed with immunohistochemistry. RESULTS: There was no expression of Bcl-2 protein in control group. The expression of Bcl-2 protein began to increase in myocytes 0.5 h after I/R in the ischemic areas and the expression of Bcl-2 protein in inner layer was stronger than in outer layer. The expression of Bcl-2 protein appears firstly in the inner layer of myocardium and extended gradually toward the outer layer 1 h after I/R. It was found that the expression of Bcl-2 protein in outer layer was stronger than in inner layer, then the expression of Bcl-2 protein became weaken gradually. CONCLUSION: Bcl-2 gene may be an important regulative gene in the process of apoptosis and the changes observed in this paper might be useful for the forensic pathological diagnosis on the early myocardial ischemia.

Animals↗

[The expression and clinical implication of inducible nitric oxide synthase mRNA in laryngeal carcinoma and hypopharyngeal carcinoma].

OBJECTIVE: To study the expression of inducible nitric oxide synthase(iNOS) mRNA in laryngeal carcinoma and hypopharyngeal carcinoma and its clinical significance. METHOD: The tissue samples (laryngeal carcinoma, 40; hypopharyngeal carcinoma, 12; adjacent tissue, 50; laryngeal papilloma, 10; amyloidosis of larynx, 2; and normal laryngeal mucosa, 7) were examined by reverse transcription-polymerase chain reaction(RT-PCR). RESULT: 1. The target gene of iNOS were detected in 41 of 52 tumor tissues, 10 of 50 adjacent tissues, 5 of 12 benign tumor tissues and 0 of 7 normal tissues. The difference was significant (P < 0.0001, P < 0.005, P < 0.0001); 2. No significant difference was found among the expression of iNOSmRNA in different primary locations, there was significant statistical difference between the early clinical stages (T1-T2) and the advanced stages (T3-T4); 3. The poorly differentiated tumor cell showed a higher iNOSmRNA expression than that in moderate differentiated tumor cell (P < 0.05) and the iNOSmRNA expression in the lymph node metastasis group was higher than the non-metastasis group (P < 0.05). CONCLUSION: It suggests that iNOS gene expression occurs in human laryngeal carcinoma and hypopharyngeal carcinoma, it might involve in the development of head and neck neoplasms in gene level by generating NO.

Aged↗

[Comparative studies on the resistance of hepatitis B by Syringa extract, IFN and ganyanling in HepG2.2.15 cells].

AIM: To explore the effects of Syringa extract, IFN and ganyanling on the the survival rate of HepG2.2.15 cells and the secretion of HBsAg and HBeAg by HepG2.2.15 cells. METHODS: (3)H-TdR incorporation and ELISA were used to detect respectively the survival rate of HepG2.2.15 cells and levels of HBsAg and HBeAg in culture supernatant of HepG2.2.15 cells after stimulation with 3 drugs. RESULTS: (1)All of the three drugs decreased the survival rate of HepG2.2.15 cells to the various extent with the increase of doses. The effect of the Syringa extract was between those of IFN and ganyanling. (2)All of the three drugs might suppress the secration of HBsAg and HbeAg by HepG2.2.15 cells in dose-dependent manner, while the the effect of the syringa extract was between those of IFN and ganyanling. CONCLUSION: The Syringa extract is an effective and low toxic anti-hepatitis B drug.

Antiviral Agents↗

Absolute configuration of tert-butyl-1-(2-methylnaphthyl)phosphine oxide.

The enantiomers of tert-butyl-1-(2-methylnaphthyl)phosphine oxide 1 have been separated using a homemade HPLC column and an analytical gradient system. Vibrational absorption and circular dichroism spectra for both enantiomers have been measured in CD2Cl2 and CH2Cl2 solutions in the 2000-900 cm(-1) region. The fully relaxed potential energy surface of (S)-tert-butyl-1-(2-methylnaphthyl)phosphine oxide, obtained using the B3LYP functional with a 6-31G basis set, indicated two stable conformers with their populations in a approximately 2:1 ratio. The vibrational absorption and VCD spectra are predicted for these two conformers using the B3LYP functional with a 6-31G basis set. The comparison of predicted and experimental spectra indicated that (+)-tert-butyl-1-(2-methylnaphthyl)phosphine oxide is in the (S)-configuration. This assignment is supported by the ab initio prediction of positive optical rotation for the most stable conformer with an (S)-configuration and the nonequivalence sense of the tert-butyl group chemical shift observed in the 1H NMR spectrum of this enantiomer measured in the presence of (+)-(S)-mandelic acid as a chiral solvating agent.

Chromatography, High Pressure Liquid↗

HIF-1alpha-prolyl hydroxylase: molecular target of nitric oxide in the hypoxic signal transduction pathway.

We have investigated inhibitory mechanisms of hypoxic activation of HIF-1alpha by nitric oxide (NO). Using a Hep3B cell-derived cell line, HRE7 cells, we found that the inhibition of HIF-1alpha activity by NO requires a substantial amount of oxygen, albeit at a lower level. We further investigated the effect of NO on the binding activity of the von Hippel-Lindau tumor suppressor protein (pVHL) to the N-terminal activation domain (NAD) overlapping the oxygen-dependent degradation domain (ODD) of HIF-1alpha, because this reaction involves prolyl hydroxylation in NAD that requires oxygen. Although we could not detect any binding activity when NAD was incubated with whole cell extracts from cells treated with CoCl(2) or desferrioxamine, the binding capacity was manifested when Hep3B cells were treated together with NO. This activation was also observed when whole cell extracts from CoCl(2)-treated cells were incubated with NO. The prolyl hydroxylase from Hep3B cells treated with CoCl(2) was partially purified about 80-fold, and several enzymatic properties were examined. The enzyme required ferrous ion and 2-oxoglutaric acid. Strong activation of the prolyl hydroxylase by NO was observed without further addition of ferrous ion.

Blotting, Western↗

Design of peptide-based inhibitors of human islet amyloid polypeptide fibrillogenesis.

Human islet amyloid polypeptide (IAPP) is the major component of amyloid deposits found in the pancreas of over 90% of all cases of type-2 diabetes. We have generated a series of overlapping hexapeptides to target an amyloidogenic region of IAPP (residues 20-29) and examined their effects on fibril assembly. Peptide fragments corresponding to SNNFGA (residues 20-25) and GAILSST (residues 24-29) were strong inhibitors of the beta-sheet transition and amyloid aggregation. Circular dichroism indicated that even at 1:1 molar ratios, these peptides maintained full-length IAPP (1-37) in a largely random coil conformation. Negative stain electron microscopy revealed that co-incubation of these peptides with IAPP resulted in the formation of only semi-fibrous aggregates and loss of the typical high density and morphology of IAPP fibrils. This inhibitory activity, particularly for the SNNFGA sequence, also correlated with a reduction in IAPP-induced cytotoxicity as determined by cell culture studies. In contrast, the peptide NFGAIL (residues 22-27) enhanced IAPP fibril formation. Conversion to the amyloidogenic beta-sheet was immediate and the accompanying fibrils were more dense and complex than IAPP alone. The remaining peptide fragments either had no detectable effects or were only weakly inhibitory. Specificity of peptide activity was illustrated by the fragments, SSNNFG and AILSST. These differed from the most active inhibitors by only a single amino acid residue but delayed the random-to-beta conformational change only when used at higher molar ratios. This study has identified internal IAPP peptide fragments which can regulate fibrillogenesis and may be of therapeutic use for the treatment of type-2 diabetes.

Amino Acid Sequence↗

Molecular regulation of iron homeostasis and resistance to infection in alcoholics.

Chronic alcohol abuse is associated with both an altered response to infection and deranged iron homeostasis. While both clinical manifestations are well known, the inter-relationships between alcohol and iron and the response to infection are not. The recent identification of a plethora of iron regulatory and transport proteins has now begun to explain these relationships. This article outlines the current state of knowledge on cellular iron homeostasis, with particular reference to the iron regulatory proteins (IRP1, IRP2 and HFE) and the iron membrane transport proteins, two of which have been shown to be members of the natural resistance- associated macrophage protein family (Nramp1 and 2). Following this introduction, the response of the body to infection, in terms of iron withholding is discussed at the cellular level, especially in terms of the macrophage and its cytokine-mediated responses. Prior alterations to body iron status are also considered in this section. The effect of alcohol alone on the body's response to infection is then outlined, principally in terms of the macrophage function and cytokine regulation. These are then combined to correlate the clinical and experimental observations with known derangements produced by the individual insults of alcohol and altered iron homeostasis. on the response to infection. Particular attention is paid not only to cytokine/chemokine actions, but also to the consequences of the altered production of reactive oxygen and nitrogen species. Finally, the possible mechanisms by which alcohol and altered iron homeostasis lead to tissue damage during infection.

Alcohol Drinking↗

Proteomic analysis of rat soleus and tibialis anterior muscle following immobilization.

A proteomic analysis was performed comparing normal slow twitch type fiber rat soleus muscle and normal fast twitch type fiber tibialis anterior muscle to immobilized soleus and tibialis anterior muscles at 0.5, 1, 2, 4, 6, 8 and 10 days post immobilization. Muscle mass measurements demonstrate mass changes throughout the period of immobilization. Proteomic analysis of normal and atrophied soleus muscle demonstrated statistically significant changes in the relative levels of 17 proteins. Proteomic analysis of normal and atrophied tibialis anterior muscle demonstrated statistically significant changes in the relative levels of 45 proteins. Protein identification using mass spectrometry was attempted for all differentially regulated proteins from both soleus and tibialis anterior muscles. Four differentially regulated soleus proteins and six differentially regulated tibialis anterior proteins were identified. The identified proteins can be grouped according to function as metabolic proteins, chaperone proteins, and contractile apparatus proteins. Together these data demonstrate that coordinated temporally regulated changes in the proteome occur during immobilization-induced atrophy in both slow twitch and fast twitch fiber type skeletal muscle.

Amino Acid Sequence↗

Theory of dipole-bound anions.

The problem of the binding of an excess electron to polar molecules and their clusters has long fascinated researchers. Although excess electrons bound to such species tend to be very extended spatially and to have little spatial overlap with the valence electrons of the neutral molecules, inclusion of electron correlation effects is essential for quantitatively describing the electron binding. The major electron correlation contribution may be viewed as a dispersion interaction between the excess electron and the electrons of the molecule or cluster. Recent work using a one-electron Drude model to describe excess electrons interacting with polar molecules is reviewed.

Journal Article↗

Proteomic analysis of rat soleus muscle undergoing hindlimb suspension-induced atrophy and reweighting hypertrophy.

A proteomic analysis was performed comparing normal rat soleus muscle to soleus muscle that had undergone either 0.5, 1, 2, 4, 7, 10 and 14 days of hindlimb suspension-induced atrophy or hindlimb suspension-induced atrophied soleus muscle that had undergone 1 hour, 8 hour, 1 day, 2 day, 4 day and 7 days of reweighting-induced hypertrophy. Muscle mass measurements demonstrated continual loss of soleus mass occurred throughout the 21 days of hindlimb suspension; following reweighting, atrophied soleus muscle mass increased dramatically between 8 hours and 1 day post reweighting. Proteomic analysis of normal and atrophied soleus muscle demonstrated statistically significant changes in the relative levels of 29 soleus proteins. Reweighting following atrophy demonstrated statistically significant changes in the relative levels of 15 soleus proteins. Protein identification using mass spectrometry was attempted for all differentially regulated proteins from both atrophied and hypertrophied soleus muscle. Five differentially regulated proteins from the hindlimb suspended atrophied soleus muscle were identified while five proteins were identified in the reweighting-induced hypertrophied soleus muscles. The identified proteins could be generally grouped together as metabolic proteins, chaperone proteins and contractile apparatus proteins. Together these data demonstrate that coordinated temporally regulated changes in the skeletal muscle proteome occur during disuse-induced soleus muscle atrophy and reweighting hypertrophy.

Amino Acid Sequence↗

Absolute configuration and conformational analysis of a degradation product of inhalation anesthetic Sevoflurane: A vibrational circular dichroism study.

1,1,1,3,3-pentafluoro-2-(fluoromethoxy)-3-methoxypropane, compound B, is a product obtained in the degradation of the anesthetic Sevoflurane. Enantiopure (+)-B was investigated using vibrational circular dichroism (VCD). Experimental absorption and VCD spectra of (+)-B in CDCl(3) solution in the 2,000-900 cm(-1) region are compared with the ab initio predictions of absorption and VCD spectra obtained from density functional theory using B3LYP/6-31G* basis set for different conformers of (S)-1,1,1,3,3-pentafluoro-2-(fluoromethoxy)-3-methoxypropane. This comparison indicates that (+)-B is of the (S)-configuration in CDCl(3) solution, in agreement with previous literature results. Our results also indicate that this compound adopts six predominant conformations in CDCl(3) solution.

Anesthetics, Inhalation↗

Sequence variations in the mu-opioid receptor gene (OPRM1) associated with human addiction to heroin.

Human mu-opioid receptor (OPRM1) is the major site for the analgesic action of most opioid drugs such as morphine, methadone and heroin. It was previously reported that a single nucleotide polymorphism (SNP) in exon1 (c.118A-->G) of OPRM1 might modestly alter the affinity in beta-endorphin-Mu interaction. Using denaturing high performance liquid chromatography (DHPLC) the complete coding region of the OPRM1 gene was screened for SNPs in Han-Chinese heroin addicts and normal control. Three novel SNPs were detected, one in exon3, one in intron3 and one in the 3' untranslated region. The SNP c.118A-->G reportedly altered the interaction of Mu receptor with opioid had no statistically significant correlation with heroin addition in Han Chinese. However, addicted subjects with the SNP in intron2 (IVS2 +31G-->A) tended to show much higher heroin intake dosages than those without this SNP. We also observed that individuals carrying both SNP c.118A-->G and IVS2 +31G-->A consumed relatively more drugs compared to other addicts. Thus our study further highlights the importance of studing the various regions of the mu opioid receptor gene, coding as well as non-coding, for genetic markers that may be linked to, or directly contribute to opioid drug-seeking behavior.

3' Untranslated Regions↗

Regulation of cardiac fibroblast cellular function by leukemia inhibitory factor.

BACKGROUND: Previous studies have shown that leukemia inhibitory factor (LIF) provokes hypertrophic and cytoprotective effects in cardiac myocytes. However, the effects of LIF in cardiac fibroblasts are not known. Given that the cardiac fibroblast is the most abundant cell type in the heart, we sought to examine the functional effects of LIF on cardiac fibroblasts in vitro. RESULTS: Short-term LIF stimulation (24h) had no effect on fibroblast proliferation and/or cell differentiation. However, longer-term LIF stimulation (48-72h) increased fibroblast proliferation, and significantly inhibited cardiac fibroblast differentiation into myofibroblasts. Moreover, 72h of LIF stimulation significantly reduced collagen content in cardiac fibroblasts cultures, as well as decreased MMP activity in fibroblast culture supernatants. CONCLUSION: The results of this study suggest that LIF stimulation down-regulates several key components of the remodeling process, including collagen content and matrix metalloproteinase (MMP) activation, and thus suggest that LIF may play an important autocrine/paracrine role in preventing excessive extracellular matrix remodeling following acute myocardial injury.

Actins↗

Mutation analysis of IgVH gene in B-cell chronic lymphocytic leukemia.

The variable heavy chain region (VH) genes of 3 untreated patients with B-cell chronic lymphocytic leukemia (B-CLL) were cloned and analyzed. The VH family used was VH3-11, VH3-72 and VH3-33. More than 2% difference from the corresponding germline gene was detected in all the 3 obtained potential functional genes (average 16.7). Mutation pattern analysis indicated evidence of antigen selective pressure observed in 1 of 3 cases. Our findings suggested that the tumor cells originate from post-GC cells.

Amino Acid Sequence↗

Preclinical and clinical assessment of the safety and potential efficacy of thalidomide in heart failure.

BACKGROUND: Inflammatory mediators, especially tumor necrosis factor (TNF), have been implicated in heart failure (HF). Thalidomide has anti-inflammatory properties and selectively inhibits TNF. Thus far, thalidomide or thalidomide analogues have not been evaluated in patients with heart failure. METHODS: Thalidomide was assessed in preclinical and clinical studies. First, isolated cardiac myocytes were pretreated with thalidomide or thalidomide analogues, and TNF production was assessed after lipopolysaccharide (LPS) provocation. Second, to determine the safety and potential efficacy of thalidomide, an open-label dose escalation safety study was conducted in seven patients with advanced heart failure. RESULTS: Thalidomide and thalidomide analogues inhibited LPS-induced TNF biosynthesis in cardiac myocytes in a dose-dependent manner. Thalidomide analogues had a greater inhibitory effect on TNF production than did thalidomide. In patients with advanced HF, thalidomide was safe and potentially effective when used at lower doses. However, dose-limiting toxicity was observed in two patients. There was a significant increase in the 6-minute walk distance and a trend toward improvement in left ventricular ejection fraction and quality of life after 12 weeks of maintenance therapy with thalidomide. CONCLUSIONS: Taken together these results suggest that thalidomide or its derivatives may be useful in selected patients with HF. This potential needs to be studied in larger clinical trials.

Adult↗