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Biomedical subjects

F Zhou

Publications and source records attributed to F Zhou.

At least 91 records · Page 5Linked to original sources
PubMed1998

[Influences of sperm quality and quantity on fertilization, cleavage rates and quality of embryos in in-vitro fertilization].

OBJECTIVE: To examine the influences of the quality and quantity of seminal sperm and inseminational sperm on in-vitro fertilization (IVF) outcomes. METHODS: chi-square tests were used to analyze the impacts of both semen and inseminated sperm concentrations and percentages of different grade motile sperm on the fertilization rates in 481 matured and 273 immatured oocytes and cleavage rate percentage of embryo with low quality in embryos obtained from 92 IVF-embryo transfer cycles. RESULTS: The fertilization rates of both kinds of oocytes and cleavage rate of fertilizaed matured oocyte significantly decreased (P < 0.05) as the concentrations and percentages of grade a and b sperms in semen reduced. The rates of embryos with abnormal morphology were significantly dropped when the above sperm parameters increased (P < 0.05). Meanwhile, it was also found that the concentration and motility of inseminated sperm could also affect the above three IVF parameters significantly (P < 0.05). CONCLUSION: Highly active semen and inseminated sperm play important roles not only in fertilization, but also in cleavage and embryo development during IVF procedure.

Cleavage Stage, Ovum↗
PubMed1998

[Determination of resibufogenin and cinobufagin in venenum Bufonis by HPLC].

OBJECTIVE: To establish a HPLC method to determine the contents of active constituents in Venenum Bufonis. METHOD: In determining the contents of resibufogenin and cinobufagin in the traditional Chinese medicine Venenum Bufonis, the mobile phase was acetonitrile-0.5% KH2PO4 solution(50:50)(pH adjusted with value phosphoric acid to 3.25 +/- 0.02). RESULT: The constituents thus determined have good linearity and separation. The average recovery of resibufogenin was 100.35%, RSD 1.86%; the average recovery of cinobufagin was 100.38%, RSD 2.09%. CONCLUSION: The method was convenient, rapid, accurate and practicable.

Amphibian Venoms↗
PubMed1998

[Microscopic identification of Herba Dianthi grown in Shandong].

The medicinal materials of four species and two varieties of Herba Dianthi grown in Shandong were identified. The result shows that they are identified easily and accurately according to the outer properties, and they are apparently divided into the Shizhu group, the Qumai group and the Dianthus shandongensis on the basis of their morphological and structural characteristics of the stem and leaf, but they have not obvious distinction among the species of every group.

Dianthus↗
PubMed1998

[Ultra-morphological study on the surface of the stem and leaf of the original plant of herba Dianthi in Shandong].

Ultra-morphology of 4 spieces and 2 varities of the original plant of Herba Dianthi grown in Shandong was identified with SEM. The result showed that they were aparently divided into the Shizhu group and the Qumai group, and the Dianthus shandongensis was between the two groups, on the basis of the cutin-grain type of the epidermis of the stem and leaf as well as the surface of the guard cell of the stoma.

Caryophyllaceae↗
PubMed1998

Identification of the cornea-specific keratin 12 promoter by in vivo particle-mediated gene transfer.

PURPOSE: Keratin 12 (K12) is a cornea epithelial cell-specific intermediate filament component. To provide a better understanding of its expression, it is necessary to identify and characterize the promoter of Krt1.12 gene. METHODS: The 2.5-kb DNA 5' to Krt1.12 gene was sequenced. Krt1.12 promoter-beta-gal DNA constructs were prepared and used in vivo to transfect rabbit corneas, conjunctivas, and skin by particle-mediated gene transfer (Gene Gun). In vitro, the DNA constructs were transfected into cultured T-antigen-transformed rabbit corneal epithelial (RCE-T) cells and human fibrosarcoma HT-1080 fibroblasts with lipofectamine. The promoter activity was assessed by measuring beta-gal (beta-galactosidase) activity using histochemical staining with 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside and enzyme assay with o-nitrophenyl beta-D-galactopyranoside. RESULTS: There are four Pax-6 pair box binding elements found between -910 and -2000 bp 5'-flanking the transcription initiation site of the Krt1.12 gene. None of promoter constricts can be expressed by HT-1080 cells. Cotransfection of Pax-6 cDNA with K12 promoter-beta-gal constructs containing Pax-6 elements results in a fourfold increase of beta-gal activities in RCE-T cells but not HT-1080 fibroblasts. The data of in vivo transfection in the rabbit by Gene Gun indicate that reporter gene constructs containing 0.6-kb and longer DNA fragments 5'-flanking Krt1.12 gene are effectively expressed in corneal, but not conjunctival or epidermal epithelial cells. CONCLUSIONS: The particle-mediated gene transfer is a suitable technique for in vivo delivery of transgenes to corneal epithelial cells. The 2.5-kb DNA fragment 5'-flanking Krt1.12 contains corneal epithelial cell-specific regulatory cis-DNA elements. Pax-6 is a positive transcription factor essential for keratin 12 expression.

Animals↗
PubMed1997

A heparin-binding synthetic peptide of heparin/heparan sulfate-interacting protein modulates blood coagulation activities.

We have previously identified and characterized a heparin-binding cell surface protein (heparin/heparan sulfate-interacting protein, or HIP) present on epithelial and endothelial cells. A synthetic peptide mimicking a heparin-binding domain of HIP is now shown to bind a small subset of heparin molecules with high affinity and, therefore, presumably recognizes a specific structural motif in the heparin molecule. Further analyses revealed that the heparin molecules exhibiting a high affinity for the HIP peptide also show an extremely high affinity for antithrombin III (AT-III), a cofactor required for heparin's anticoagulant activity. The HIP peptide was shown to compete with AT-III for binding to heparin and to neutralize the anticoagulant activity of heparin in blood plasma assays. Furthermore, the heparin subfraction that binds to the HIP peptide with high affinity exhibits an extremely high anticoagulant activity. We conclude that although the HIP peptide shows no sequence similarity with AT-III, the two proteins recognize the same or similar structural motifs in heparin.

Amino Acid Sequence↗
PubMed1997

[Idiopathic rapidly progressive glomerulonephritis and small vessel vasculitis].

We analyzed the clinical and pathological characteristics of patients with idiopathic rapidly progressive glomerulonephritis (idiopathic RPGN), who had been hospitalized in recent three years. Five out of 8 patients with idiopathic RPGN were positive for anti-neutrophil cytoplasmic autoantibodies (ANCA), most of them showed pANCA by indirect immunofluorescence technique. Compared with those who were ANCA negative, ANCA positive patients had an elder onset age; most of them had fever, arthragia, myalgia, skin rash, and some patients also had deafness, eye and nasal disorders. ANCA positive patients had a positive CRP, elevated gammaglobulins and accelerated ESR (> 100 mm/1 h). Typical pathological findings for ANCA positive patients were focal segmental fibroid necrosis of the glomerular capillary tufts and diffuse crescents formation. After an intensive therapy, the renal functions of most ANCA positive patients were obviously improved and most of them became hemodialysis independent. The results suggested that idiopathic RPGN might be a renal limited form of small vessel vasculitis and the patients with idiopathic RPGN had a better prognosis. Early diagnosis and appropriate intensive therapy are critical for patients with idiopathic RPGN.

Adult↗
PubMed1997

[Detection of p53 gene mutations in hepatocellular carcinoma].

This study screened 32 cases of hepatocellular carcinoma (HCC) from Chengdu detecting HBV DNA. HBsAg and p53 mutations by using Southern blot hybridization, immunohistochemistry and polymerase chain reaction/restriction enzyme digest methods, respectively. The results revealed all the cases had been infected by HBV; the frequency of HBV DNA integration into HCC cell was 72%, and the positive staining for HBsAg 96.3%(26/27). Of the 32 cases, 8 showed nuclear staining of p53 protein (25%), no mutation of p53 gene at all in 27 nontumorours liver tissues was identified. The resluts suggest the inactivation of p53 function may play a significant role in the genesis of HBV-associated HCC; however, the largely negative p53 mutation results in the current study conversely indicate that hepatocarcinogenesis may even involve other comprehensive mechanisms. Further studies are worth doing to evaluate the possible contribution of HBV to the p53 mutation in HCC.

Adult↗
PubMed1997

[Investigation and identification of original plants of Herba Dianthi produced in Shandong].

The original plant of Herba Dianthi in Shandong are divided into 4 species and 2 varieties by investigation and classified identification. Of them Dianthus shandongensis J. X. Li et F. Q. Zhou is a new species and D. chinensis L. var. versicolor (Fich. ex Link) Y. C. Ma is a new recorder in Shandong. D. chinensis L. is the most important source of Herba Dianthi in Shandong and the D. chinensis L. var. versicolor and D. chinensis L. var. liaotungensis Y. C. Chu, occasionally is D. shandongensis, D. superbus L. and D. longicalyx Miq. Haven't grown into commercial medicinal materials.

Dianthus↗
PubMed1997

Isolation and identification of berberine from cell cultures of Coptis chinensis.

Calli were formed by the young leaves of Coptis chinensis inoculated onto a 6,7-V solid medium containing 1.0-2.0 mg/L of 2,4-D and 0.1 mg/L of kinetin. The loose calli were chosen and transferred into a liquid medium and free cells and cell aggregates were obtained. Cell lines with a higher content of berberine were selected by irradiation of suspension culture and a plate-screening technique. After 35 subcultures, the selected cell lines were cultured in a larger quantity for the extraction of alkaloids. Yellow crystals were obtained from the extracts and identified as berberine by TCL, UV and IR absorption, and mass spectrography. They have the same molecular structure and anti-bacterium activities as the berberine obtained from natural plants.

Berberine↗
PubMed1997

Familial occurrence of hemangioblastoma of central nervous system.

OBJECTIVE: To study the familial occurrence of hemangioblastoma of the central nervous system and to search for an effective way to diagnose and manage the disease. METHODS: From 1974 to 1993, six patients with hemangioblastoma with family history underwent surgical treatment at our hospital. Computerized tomography (CT) or magnetic resonance imaging (MRI) technique was used for the diagnoses before operation. RESULTS: Each patient had more than two relatives with hemangioblastoma. Multiple lesions were found in two of the six familial hemangioblastoma cases. All the patients recovered well after operation. So far, no victims have been discovered in the third generation. CONCLUSIONS: The high occurrence rate of familial hemangioblastoma cases suggests an involvement of hereditary factors. We need a long follow-up study on the descendants of familial hemangioblastoma patients. MRI is of diagnostic value in determining the location and nature of hemangioblastoma in both brain and spinal cord. A thorough examination is necessary for hemangioblastoma patients, and microsurgery is important in removing the tumor.

Adult↗
PubMed1996

Pleiotropic defects in ataxia-telangiectasia protein-deficient mice.

We have generated a mouse model for ataxia-telangiectasia by using gene targeting to generate mice that do not express the Atm protein. Atm-deficient mice are retarded in growth, do not produce mature sperm, and exhibit severe defects in T cell maturation while going on to develop thymomas. Atm-deficient fibroblasts grow poorly in culture and display a high level of double-stranded chromosome breaks. Atm-deficient thymocytes undergo spontaneous apoptosis in vitro significantly more than controls. Atm-deficient mice then exhibit many of the same symptoms found in ataxia-telangiectasia patients and in cells derived from them. Furthermore, we demonstrate that the Atm protein exists as two discrete molecular species, and that loss of one or of both of these can lead to the development of the disease.

Animals↗