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Biomedical subjects

F Zhang

Publications and source records attributed to F Zhang.

At least 523 records · Page 29Linked to original sources
PubMed1995

[Effects of scopolamine on serotonin in the spinal cord and atria of morphine-dependent rats].

The effects of scopolamine on serotonin (5-HT) in the spinal cord and atria of morphine-dependent rats were studied. Levels of 5-HT and its metabolite, 5-hydroxyindolactic acid (5-HIAA) in the spinal cord and atria were analyzed by HPLC-ECD method. The results showed that contents of 5-HT, 5-HIAA in the spinal cord of morphine-dependent rats were statistically higher than those of controls (P < 0.01, n = 6), and that scopolamine inhibited morphine's effect by decreasing 5-HT, 5-HIAA in the spinal cord. After acute abstinence (1d), the contents of 5-HT in the spinal cord in natural withdrawal group were decreased, but increased in scopolamine group. After chronic abstinence (6d), the contents of 5-HT in the spinal cords in the two groups were all increased to above normal level. The results also showed that scopolamine did not affect the increasing of 5-HT in atria induced by morphine. The data support a role of muscarinic receptor on changes of 5-HT metabolism in the spinal cord in morphine-dependent rats.

Animals↗
PubMed1995

[Surgical treatment of penoscrotal transposition associated hypospadias].

Thirty-four patients with penoscrotal transposition associated with hypospadias were surgically treated from 1978 to 1992. Penoscrotal hypospadias was noted in 23, and perineal hypospadias in 11. Incomplete penoscrotal transposition was scen in 24 patients, and complete penoscrotal transposition in 10. 32 patients out of 34 obtained satisfactory results after the correction of penoscrotal transposition. Complication occurred in 5 patients (urethral fisula 3 and orifice structure 2). The operative procedures of penoscrotal transposition were discussed.

Abnormalities, Multiple↗
PubMed1995

Protective effect of radix Salviae Miltiorrhizae Composita on cerebral ischemia.

The protective effects of Radix Salviae Miltiorrhizae Composita (RSMC) on cerebral ischemia in gerbils produced by unilateral ligation of left common carotid artery and on the development of ischemic cerebral edema were studied. It was found that the administration of RSMC resulted in a very significant decrease in neurological deficits and the time for exhibiting first signs of neurological deficit delayed compared with untreated animals. Although the values of specific gravity of fresh cerebral tissue on the ligated side of common carotid artery in untreated animals (1.0406 +/- 0.0039 SD) and in RSMC-treated animals (1.0453 +/- 0.0009) were decreased significantly compared to that (1.0468 +/- 0.0003) of sham-operated animals, the specific gravity of cerebral tissue in RSMC-treated animals was significantly heavier than that in untreated animals (t = 5.5022, P < 0.001), i.e. the administration of RSMC decreased the severity of cerebral edema. A significant negative correlation was found between stroke index and specific gravity, and it suggests that one of the protective mechanisms of RSMC might be related to its ameliorating cerebral edema.

Animals↗
PubMed1995

[Surgical treatment of traumatic cataract].

140 cases and 142 eyes with traumatic cataracts treated by extracapsular cataract extraction (ECCE), ECCE and intraocular lens implantation (ECCE + IOL), and lensectomy were reported. The data showed that postoperative corrected visual acuities > or = 0.1-1.0 were in 89 eyes (70%) in one week and in 33 eyes of 44 eyes (75%) followed up for more than 6 months. The 3 methods of operation were compared, the indications and the optimum time of operation for each method and the personal experiences of the authors in the operation were discussed.

Adolescent↗
PubMed1995

[Processing of pyritum from different sources].

Studies on the processing of Pyritum from different source were conducted by means of indentical technological procedure using total sulphur, S,FeS2 and Fe7S8 as indexes. The results showed considerable differences in compositions of the processed products.

Hot Temperature↗
PubMed1995

Anti-inflammatory effects of tianrong acupoint on blood vessels of dura mater.

Although Tianrong acupoint (SI 17) is used for migraine headache, its mechanism of action remains obscure. The effects of Tianrong acupoint therapy on neurogenically-mediated plasma protein extravasation (125I-BSA) in rat dura mater induced by electrical stimulation of the right trigeminal ganglion was studied. When the unilateral trigeminal ganglion was stimulated (5 Hz, 1.2 mA, 5 msec for 5 min), the ratio of stimulated side/unstimulated side (cpm/mg) was 1.6663 +/- 0.0217. The plasma extravasation was blocked by different kinds of treatment in different degrees. The order of the ratio was as follow: Tianrong acupoint therapy (1.0917 +/- 0.0266) and Tianrong electrical needle therapy (1.1281 +/- 0.0227) < Tianrong acupoint injection with normal saline (1.325 +/- 0.0444) < Quchi acupoint (LI 11) injection with prednisolonum (1.5284 +/- 1.1624). The results indicated that Tianrong acupoint therapy could inhibit the neurongenic inflammation on the affected side.

Acupuncture Points↗
PubMed1995

[Molecular cloning of cellulase gene from the bacillus].

The CMCase gene from the Bacillus sp. strain E2 was cloned into E. coli DH5 alpha F' using plasmid pBR322 as a vector. Recombinant plasmid pBG3 was isolated from transformants producing CMCase, and the cloned CMCase gene was found to be in a 4.0kb HindIII fragment. The CMCase gene was subcloned in a functional state on a 2.4kb DNA fragment. The optimum pH and temperature for activity of CMCase encoded by pBG3 were pH 6.5 and 55 degrees C respectively. The CMCase could not hydrolyze any natural cellulose examined. Eighty-three percent of the CMCase was detected in the inner cellular fraction, 9% and 8% were in the periplasmic space and medium respectively. Result of Southern hybridization indicates that the cloned DNA fragment was derived from Bacillus sp. strain E2 chromosomal DNA.

Bacillus↗
PubMed1995

[Effects of electroacupuncture on gastrin, mast cell and gastric mucosal barrier in the course of protecting rat stress peptic ulcer].

The experimental Wistar rats were divided into two groups, the acupuncture group and the control group. Stress-induced gastric ulcer models were established by immersion of restrained rats in water. The electroacupuncture was able to protect stress rat from stress induced peptic ulcer. The results indicated that electroacupuncture protecting rat from stress ulceration was relevant to enhancing gastric mucosal barrier, stabilizing gastric mast cell and inhibiting the gastrin levels in gastric mucous.

Animals↗
PubMed1995

[Influence of acupuncture on blood pressure, contents of NE, DA and 5-HT of SHR and the interrelation between blood pressure and whole blood viscosity].

Twenty spontaneous hypertensive rats (SHR) with systolic blood pressure (SBP) >or= 20 kPa were chosen and divided randomly into two groups (acupuncture group and control group). The former was acupunctured on bilateral Quchi (L1 11), Zusanli (ST 36). Another 46 immature SHRs which were about 7-week-old with SBP ranged from 16 kPa to 20 kPa were given 10% high molecular weight dextran (HMWD) or normal saline by intervention respectively. The results showed: 1. After three course of acupuncture, SHRs' SBP dropped remarkably from 25.493 +/- 0.73 kPa to 19.547 +/- 0.555 kPa (P < 0.01); 2. After three courses of acupuncture, NE and 5-HT contents in plasma were lowered (P < 0.05, < 0.01) and DA content in plasma was increased (P < 0.05) compared with that in the control group. 3. After three course of acupuncture, NE content in brainstem, hypothalamus, cerebral cortex increased (P < 0.005-0.01) and DA content in brainstem, cerebral cortex increased remarkably (P < 0.005-0.01) compared with that in the control group. 5-HT content in brainstem, hypothalamus, and cerebral cortex increased obviously compared with that in the control group. 4. After 1 or 2 hours of intervention of 10% HMWD to immature SHR, the whole blood viscosity and blood pressure rise remarkably compared with normal saline group. The experiment implies that the mechanism of lowering the blood pressure of SHR by acupuncture lies in that it can adjust NE, DA and 5-HT contents in plasma and central nervous system to adjust the activity of sympathetic, reduce whole blood viscosity etc.

Animals↗
PubMed1995

[Familial exudative vitreoretinopathy].

Familial exudative vitreoretinopathy (FEVR) is an autosomal dominant inherited disease. The developmental abnormality of retinal vessels is the basis of various lesions in FEVR. 11 affected members (21 eyes) from 5 families with FEVR were studied. The emphasis was placed on describing the changes of fundus and fundus fluorescein angiography of the disease. Argon laser photocoagulation was performed on 8 patients (14 eyes) and they were followed for 5 approximately 14 months. The diagnosis, differential diagnosis, complications and treatment of FEVR were discussed with some materials in this paper.

Adolescent↗
PubMed1995

Acute hypoxic vasoconstriction in isolated rat small and large pulmonary arteries.

Hypoxic vasoconstriction (HPV) has been shown to consist of a biphasic contraction change. The first phase of the hypoxic response peaks at approximately five minutes. The second phase is at about 30 minutes. The force of contraction of both phases of HPV were found to be significantly greater in pulmonary resistance vessels (PRV) than in pulmonary artery (PA) (P < 0.01). The endothelium modulates the hypoxic response, especially of the second phase of HPV (68% reduction in PRV) (P < 0.05). In Ca(2+)-free solution, the first peak and the second peak of HPV were reduced to 11 and 32% contraction in PRV and to 26 and 21% contraction in PA. A calcium channel antagonist (amlodipine) caused significant dose-dependent inhibition of the first phase of HPV (P = 0.001), with a significantly greater effect on PRV compared to PA (P < 0.01). Levcromakalim caused a dose-dependent inhibition of HPV in PRV (58% at 10 microM). In contrast, HPV in PA was not significantly inhibited by levcromakalim. In conclusion, this study has confirmed that hypoxia induces a biphasic contractile response in isolated pulmonary arteries requiring extracellular calcium. Both amlodipine and levcromakalim inhibit hypoxic pulmonary vasoconstriction and these agents may be of value in the treatment of pulmonary hypertension.

Acute Disease↗
PubMed1994

A new staging system for nasopharyngeal carcinoma in China.

PURPOSE: An accurate and rational nasopharyngeal carcinoma (NPC) stage based on images is proposed. METHODS AND MATERIALS: Four hundred and twenty-one cases of NPC, treated in the Cancer Hospital, Sun Yat-sen University of Medical Sciences (SUMS), with computed tomography scanning before initial radiotherapy, are analyzed. Important prognostic factors that form the basis of the new staging system are screened out by means of Cox model and clinical experiences. Survival curves of various kinds of T and N stages are compared by computer simulation. A new staging system is proposed by studying the traditional staging systems such as the AJC, UICC, Ho's and Changsha systems. RESULTS: According to the new staging criteria, the 5-year survival rates for Stages I-IV are 89.7%, 75.9%, 51.3%, and 22.2%, respectively. CONCLUSION: This new clinical staging for NPC based on a large amount of cases multivariate analysis is satisfactory and widely used in China.

Adult↗
PubMed1994

Calorimetric studies of the interactions of cytochrome c with dioleoylphosphatidylglycerol extruded vesicles: ionic strength effects.

Cytochrome c has been studied as an example of a peripheral membrane protein which interacts with the lipids as well as the proteins of the inner mitochondrial membrane. In order to elucidate the thermodynamic properties of these interactions, isothermal titration calorimetry and differential scanning calorimetry (DSC) were used to study the binding of cytochrome c to negatively charged dioleoylphosphatidylglycerol (DOPG) extruded vesicles as a function of ionic strength. The binding constant and enthalpy of association decrease with increasing ionic strength, with no binding detected above 0.5 M NaCl. The enthalpy of the binding of cytochrome c to DOPG-extruded vesicles was 15 kcal/mol, and the binding constant was 6 x 10(6) M-1 at the lowest ionic strengths. The minimum size of the lipid cluster to which the protein bound was found to be approx. 9 lipid molecules in the titration calorimetry measurements and as low as 5 lipid molecules in the DSC measurements. The stability of the bound cytochrome c was found to be reduced; the thermal denaturation temperature was lowered from 83 to 50 degrees when bound to DOPG. The results of this study support previous suggestions that cytochrome c may undergo a conformational change when it binds to charged lipids such as DOPG. The results also support the suggestion that the protein penetrates partially into the lipid bilayer.

Calorimetry, Differential Scanning↗
PubMed1994

Distribution of immediate early gene zif-268, c-fos, c-jun and jun-D mRNAs in the adult cat with special references to brain region related to vision.

The distribution of immediate early gene zif-268, c-fos, c-jun and jun-D mRNAs was investigated in the visual cortex, dorsal lateral geniculate nucleus and hippocampus of the adult cat brain with in situ hybridization. In area 17, zif-268, c-jun and jun-D were found predominantly in layers II-III and VI, while c-fos mRNA was abundant in layer VI. In area 18, the zif-268, c-fos and c-jun labelling pattern was identical to that of area 17, this was not true for jun-D. In area 19, only c-jun retained the lamination pattern of areas 17 and 18, while zif-268, c-fos and jun-D were homogeneously distributed. In the dorsal lateral geniculate nucleus, only c-fos and jun-D resulted in labelling. In the pyramidal layer of hippocampus, zif-268 was found in CA1-4, c-jun in CA1-3, and jun-D in CA2-4. In the dentate gyrus, c-jun was abundant, jun-D moderate and zif-268 faint. C-fos labelling was absent in the hippocampal formation.

Animals↗
PubMed1994

Probing the structure of bacteriophage phi 29 prohead RNA with specific mutations.

Bacteriophage phi 29 of Bacillus subtilis packages its double-stranded DNA genome into a preformed prohead in an ATP-dependent reaction. A 174-residue phi 29-encoded RNA molecule (pRNA) is a structural component of the prohead and is essential for DNA packaging. The secondary and tertiary structures of the prohead binding site on pRNA have been probed using a series of specific mutant pRNAs and by measuring binding to RNA-free proheads and in vitro packaging of the DNA-gene product 3 (DNA.gp3) complex. A pseudoknot in pRNA inferred from phylogenetic studies was confirmed with specific mutations, and this pseudoknot was necessary for DNA.gp3 packaging activity. pRNA was truncated progressively from the 5' and 3' ends to isolate the prohead binding site, and three truncated pRNAs of 79, 71, and 62 residues retained prohead binding activity but could not reconstitute proheads for DNA.gp3 packaging. Mutation resulting in changes of the D hairpin loop and its connecting residues within the prohead binding site of pRNA and DNA packaging studies demonstrated that some alteration of secondary structure in this helix was permissible. The analyses provided further confirmation of a discrete prohead binding domain in pRNA and further delineated specific structural requirements for DNA.gp3 packaging activity which may not be required for prohead binding.

Bacillus Phages↗