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Biomedical subjects

F Yoshimura

Publications and source records attributed to F Yoshimura.

At least 73 records · Page 4Linked to original sources
PubMed1983

Pituitary folliculo-stellate cells immunostained with S-100 protein antiserum in postnatal, castrated and thyroidectomized rats.

Pituitary folliculo-stellate cells and associated cells were studied immunohistochemically throughout the life of male and female rats. The marginal layer cells of the pars distalis and intermedia as well as the folliculo-stellate cells were immunostained with anti-S 100 protein serum. The immunostained folliculo-stellate cells in one section were identified as themselves by their ultrastructural properties on the adjacent section. Corticotrophs, thyrotrophs and prolactin cells, characterized by the stellate shape, were not immunostained with anti-S 100 protein serum. Reactivity for S-100 protein appeared on postnatal Day 6 in the marginal layer cells of the pars intermedia, and appeared on Day 10 in those cells of the pars distalis. No immunostained folliculo-stellate cells appeared before Day 6, but a few of them appeared on Day 10. Thereafter they increased in number, having more intense immunostaining with advancing age. In castrated rats, the immunostained folliculo-stellate cells appeared quite numerously, with branched cytoplasmic processes surrounding the gonadotrophs. In the thyroidectomized rats, however, folliculo-stellate cells lacked ramified cytoplasmic processes, and their topographic affinity for thyrotrophs was negligible.

Age Factors↗
PubMed1982

Fine structural criteria of prolactin cells identified immunohistochemically in the male rat.

Immunoreactive prolactin (PRL) cells in the adult male rat pituitary were observed by light microscopy to be scattered throughout the gland without special localization but sometimes to form small clusters consisting of five to ten cells. The cells had oval, polygonal, and cuplike shapes. Using the "superimposition technique," the fine structural properties of the PRL cells were examined on ultrathin sections just adjacent to the thick plastic section for immunostaining. Four cell types were distinguished: (1) oval, polygonal, and elongate cells with only small spherical granules, 130-200 nm in diameter; (2) oval or polygonal cells with both medium-sized spherical granules (250-300 nm) and about same size of polymorphic granules; (3) polygonal cells containing only large polymorphic granules (300-700 nm in maximal diameter); (4) cup-shaped PRL cells with spherical and small polymorphic granules. Furthermore, the prolactin immunoreactivity of these cell types was confirmed by the electron immunohistochemistry. Type 1 cells resemble, in fine structure, Kurosumi-Oota LH-gonadotrophs, but the former are not stained with anti-rat LH beta serum, but with anit-rat PRL serum. Although the functional relationship between these four types of cells is still unclear, it is concluded that the polymorphic shape of the granules is not necessarily an absolute criterion for identification of the PRL cell in the male.

Animals↗
PubMed1982

Fine structural criteria for pituitary thyrotrophs in immature and mature rats.

Fine structural criteria for identifying thyroid-stimulating hormone (TSH) cells in immature and mature rats have been studied by a modified superimposition technique. On days 10 and 20, some small oval immature TSH cells are scattered individually throughout the glandular tissue with a peripheral immunoreactive rim resulting from the sparse distribution of minute secretory granules less than 50 nm in diameter. The immunostained stellate TSH cells are clustered and have secretory granules 50-100 nm in diameter at the cell margins. On day 60, a few small immature TSH cells still remain. Although a few polygonal TSH cells that may not fully mature accumulate secretory granules 100-150 nm in diameter at the cell margins, the majority of TSH cells take the form of large stellate cells filled with secretory granules with the corresponding diameter, and surround an acidophil. These stellate TSH cells are characterized by dense arrangement of parallel arrays of rough endoplasmic reticulum (rER) or rER cisternae. The clustered or isolated elongate TSH cells are also observed to be vesiculated and to have numerous secretory granules 150-250 nm in diameter. In addition, large oval vesiculated TSH cells storing numerous secretory granules 150-250 nm in diameter appear sporadically in the gland, ultrastructurally resembling the gonadotrophs. It is concluded that the rat TSH cell is not a single type with a particular ultrastructure, but modifies its morphology according to its maturation or functional phase.

Aging↗
PubMed1982

Immunohistochemical and electron microscopical studies of mitotic adenohypophysial cells in different ages of rats.

Mitotic rates of the six types of immunohistochemically identifiable adenohypophysial cells were histometrically calculated in colchicine-pretreated male rats 5, 17, 30 and 70 days old. Sections were stained with the antisera against rLH, rFSH, rTSH, oGH, rPRL and pACTH1-39. The mitotic growth rate of the anterior pituitary gland at 30 days of age was much higher than at other times. Mitotic growth rates of GH and PRL cells increased with advancing age, while those of ACTH-TSH- and immunonegative cells decreased with advancing age. LH/FSH cells showed no variation in mitotic growth rate with age. Mitotic cells can be classified into six cell types based on their fine structural properties: (1) agranular cells associated with the folliculo-stellate cells; (2) ambiguous cells with scanty minute secretory granules (50-150 nm in diameter); (3) basophils with a number of small secretory granules (130-200 nm); (4) immature acidophils whose large secretory granules (130-300 nm) are sporadically scattered; (5) acidophils with numerous spherical larger secretory granules (200-300 nm); and (6) prolactin cells with large polymorphic granules. At day 5 there was a high mitotic rate of the agranular and ambiguous cells [types (1) and (2)]; at day 70 a high mitotic rate was found in immature and mature acidophils [types (4) and (5)]. The mitotic rate of basophils (type 3) was high only at day 17 and low at all other times. The mitotic rate of prolactin cells (type 6) showed a slight increment with advancing age. It is concluded that the mitotic rates of the six cell types are age-dependent.

Aging↗
PubMed1982

Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes.

Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydrolysis of cephacetrile, cephaloridine, and various phosphate esters by hydrolytic enzymes located in the periplasm. The permeability to these compounds was about 100-fold lower than in the outer membrane of Escherichia coli K-12. Also, we found that the apparent Km values for active transport of various carbon and energy source compounds were typically higher than 20 microM in P. aeruginosa, in contrast to E. coli in which the values are usually lower than 5 microM. These results also are consistent with the notion that the P. aeruginosa outer membrane indeed has a low permeability to most hydrophilic compounds and that this membrane acts as a rate limiting step in active transport processes with high Vmax values.

Cell Membrane Permeability↗
PubMed1981

Interaction of vanadate with membrane-bound ATPase from Mycobacterium phlei.

Vanadate inhibited the formation of proton gradient and membrane potential as well as Ca2+ transport by everted membrane vesicles from Mycobacterium phlei, with half-maximal inhibition occurring at 5 to 14 microM. That this is due to the inhibition of the proton-translocating ATPase was suggested by the observation that the inhibition described above occurred only when the processes were driven by the hydrolysis of ATP but not when energized by the oxidation of succinate and NADH. Furthermore, vanadate did indeed inhibit ATP hydrolysis by these membrane vesicles. Although the inhibition of ATP hydrolysis could be demonstrated only in the presence of high concentrations (e.g. 11 mM) of Mg2+, this was presumably due to the fact that we were measuring the sum of ATP hydrolysis by both coupled and partially uncoupled enzymes. This is the first reported effect of vanadate on bacterial proton-translocating ATPase.

Adenosine Triphosphatases↗
PubMed1981

Fine structural criteria for identifying rat corticotrophs.

The fine structural characteristics of normal rat corticotrophs stained with anti-porcine ACTH1-39 serum were studied. At the ultrastructure level immunoreactive corticotrophs appear to comprise four distinct cell types: (1) large stellate cells (Siperstein cells) containing granules (170-250 nm in diameter) arranged in a peripheral row and usually embracing an acidophil; (2) elongate spindle-shaped cells (Moriarty cells) in which the secretory granules (170-250 nm in diameter) are distributed in a row or in small clusters in the peripheral cytoplasm: (3) oval or polygonal cells filled only with small secretory granules (130-170 nm in diameter), resembling the "acidophil of small granules type" (Yoshimura et al. 1974); and (4) polygonal or stellate cells filled with secretory granules of varying diameters (180-300 nm in diameter) and occasionally embracing an acidophil. The first type is the most common, but the others are infrequent. It is concluded that the criteria of Siperstein and Miller (1970) do not necessarily include all categories of rat corticotrophs.

Adrenocorticotropic Hormone↗
PubMed1981

A whole range of fine structural criteria for immunohistochemically identified LH cells in rats.

Pituitaries from normal, young and adult male rats were fixed either in sublimate-formalin or in glutaraldehyde-osmium. In adjacent Paraplast sections, almost all the gonadotrophs were immunostained with both LH and FSH antisera. The rat LH beta and FSH antisera used were shown to be highly specific by the absorption test and by double antibody radioimmunoassay. Thin and thick adjacent Epon sections were prepared for EM and immunohistochemical examination. Cells stained with the rat LH beta antiserum were identified by LM, and the observed in detail by EM. On the basis of these observations we suggest that the LH cells are arranged in a sequence of basophils, i.e., Types II/III, III, III/IV and IV: Type II/III basophils are elongate with a cytoplasmic process and less vesiculated. They have morphological features of Type II (classical thyrotrophs) and also of Type III basophils. Type III basophils are oval in shape and moderately vesiculated. Both Types II/III and III basophils can be divided into two classes of cell characterized mainly by the existence of only small secretory granules (150-220 nm in diameter) (Type A) or by the coexistence of small and large (350-500 nm) (Type B). Type III/IV basophils are cells intermediate between types III and IV basophils, and moderately vesiculated with an abundance of secretory granules (150-300 nm in diameter). Type IV basophils are large, spherical or oval cells whose RER cisternae are conspicuously dilated; they contain less numerous secretory granules (150-300 nm in diameter). It is concluded that LH cells are not a single cell type, but include a wide range of subtypes.

Animals↗
PubMed1981

Immunohistochemical study of the postnatal development of pituitary thyrotrophs in the rat, with special reference to cluster formation.

The postnatal development of rat pituitary thyrotrophs was investigated immunohistochemically on days 1, 3, 5, 10, 15 and 25. Fetal thyrotrophs are strongly immunoreactive. In the postnatal period, however, weakly immunoreactive thyrotrophs increase in number to constitute clusters on days 3--5. The numbers and dimensions of the clusters reach a maximum on day 10. Thereafter the clusters break down to give rise to single, scattered neogenic thyrotrophs. Thyrotrophs in clusters on day 10 were investigated by electron microscopy in adjacent sections. They can be characterized as an immature type of basophil, according to the classification of Yoshimura et al. (1977): 1) Type I basophils, which are irregularly shaped with elongate processes, and characterized by rows of secretory granules about 100 nm in diameter. 2) Type I/II basophils, i.e., forms intermediate between Types I and II, containing less numerous secretory granules about 100--150 nm in diameter. Type II basophils which correspond to the classical thyrotrophs are not fully developed on day 10. Thus, most thyrotrophs develop from the clusters in the neonatal period. Such neogenic thyrotrophs retain the immature characteristics of Type I and I/II cells and may develop into Type II cells during subsequent maturation.

Animals↗
PubMed1980

Prolactin immunoreactivity of acidophils of the small granule type.

The fine structure of some oval anterior pituitary cells of the adult male rats immunostained with an antiserum to rat prolactin was investigated electron microscopically on the adjacent thin sections. Their fine structural appearance is identical with that of acidophils of the small granule type (Yoshimura et al. 1974) resembling the Kurosumi-Oota LH gonadotrophs. The secretory granules of the oval cells are spherical in shape, ranging from 130 to 200 nm in diameter. Large polymorphic granules, which are generally believed to be characteristic of prolactin cells, are absent from their cytoplasm. It is concluded that the acidophil of the small granule type with a similar fine structure to the Kurosumi-Oota LH gonadotroph is a prolactin secreting cell.

Animals↗
PubMed1980

Acetate kinase in the genus Veillonella: effect of succinate, serological cross-reactivity, and separation by electrophoresis.

Acetate kinases from the genus Veillonella were divided into two types: a succinate-stimulated enzyme and a succinate-independent enzyme. Three strains, V. parvula ATCC 17743 (antigenic group II), V. parvula ATCC 17744 (V), and V. parvula ATCC 10790 (VI), contained the succinate-stimulated enzyme. Among four types strains of V. alcalescens, three strains, ATCC 17747 (I), ATCC 17746 (III), and ATCC 17748 (VII), contained the succinate-independent enzyme, whereas only one strain, ATCC 17745 (IV), contained the succinate-stimulated enzyme. Small amounts of antiserum to the purified acetate kinase from V. alcalescens ATCC 17748 completely inhibited the purified and crude enzyme activity from the strain. Classification of the enzymes on the basis of stimulation by succinate was consistent with classification based on serological reactions using the antiserum as an independent parameter. The succinate-stimulated enzyme could be separated into two classes according to the degree of sensitivity to succinate: (i) enzymes from V. parvula ATCC 17744 and V. alcalescens ATCC 17745, which could be demonstrated on gel after electrophoresis by a histochemical method to be highly stimulated by the presence of succinate in the reaction mixture, and (ii) enzymes from V. parvula ATCC 10790 and V. parvula ATCC 17743, which could be easily demonstrated without succinate. Four groups of acetate kinases from the genus Veillonella were separated by gel electrophoretic mobility. The results showed that almost all enzymes from the seven type strains were heterogeneous at the molecular level.

Acetate Kinase↗
PubMed1980

Various types of the pituitary folliculo-stellate cells involving the Siperstein's corticotroph in the normal rats.

The differentiation of the folliculo-stellate (F-S) cells was electronmicroscopically investigated in the normal male adult rats from the Wistar, Wistar-Imamichi, Holzmann, Spraque-Dowley and Donryu strains. The F-S cells may be divided into the five types according to the granulation. Each type is, however, provided with the common characteristic features, i.e., the stellate shape due to projecting the cytoplasmic processes and a tendency to embrace an acidophil. The first type is an agranular independent or anastomosing immature cell. It is different in shape and arrangement from the follicular cell, but similar in agranularity and immaturity to it. The second is a slightly differentiated cell, in which scanty small secretory granules 50--100 nm in diameter begin to appear near the plasma membrane. The third is a moderately differentiated cell providing the regularly row arrangement of secretory granules 100--200 nm in diameter along the plasma membrane, corresponding, in fine structure, with a corticotroph. The fourth is a fully differentiated cell with heavy granulation, whose secretory granules 150--250 nm in diameter are accumulated in the whole cytoplasm, suggesting the storing type. It is difficult to determine whether the fourth type coincides with a hypergranulated corticotroph or a stellate thyrotroph. The fifth is a kind of fully differenetiated cell which may refer to the releasing phase of the fourth type, being characterized by the dispersion or loss of minute secretory granules of low density as large as 50 nm in diameter, associated with the cored vesicles. The population densith of the above five types increased in the sequence, 5th leads to 4th leads to 1st leads to 3rd type in the gland. Namely, the 3rd (corticotroph) type and 1st (agranular) type are predominantly distributed, and the 5th (releasing) type and 4th (hypergranulated) type are rarely.

Adrenocorticotropic Hormone↗