Biomedical subjects

F Yokoya

Publications and source records attributed to F Yokoya.

3 recordsLinked to original sources

PubMed1986

Estimation of chicken microbial load using the growth curve parameters of chicken microflora.

Chicken microbial loads, estimated through absorbance increase of culture medium inoculated with the contaminant microflora of the carcasses, were compared with total plate counts and psychrotrophic counts obtained on the same carcasses using the pour plate method after 0, 48, 96, and 144 hr stored chicken at 4 to 5 C. For estimating microbial loads on the carcasses, the mathematical relation 1n AO = 1nA1 - R - Kt was used, which was developed by combining the growth and R equations (described in Materials and Methods) and using growth data at 28 C. The values obtained by this method, when compared with those of plating, give correlation coefficients of .94, .91, .88, and .64 for total plate counts after 0, 48, 96, and 144 hr of storage and .94, .83, .82, and .86 for psychrotrophics counts after 0, 48, 96, and 144 hr of cold storage. The method proposed in the present work permits the estimation of psychrotrophics and total counts in no more than 11 hr, which is very promising for industrial applications.

Animals↗

PubMed1975

Method for sampling meat surfaces.

A new method for sampling meat surfaces was developed. Bacterial counts of beef carcasses by the cotton swab technique and by the new method showed that the latter gave higher counts. These counts were closely correlated with data obtained by using the swab method. Advantages of the new method are its simplicity, rapidity, and adaptability to routine use on any type of carcass.

Animals↗

PubMedDate not supplied

Species differences in the metabolism of suprofen in laboratory animals and man.

The metabolism of the oral anti-inflammatory agent suprofen (S), 2-4-(2-thienylcarbonyl)phenyl)propionic acid, has been studied in mice, rats, guinea pigs, dogs, monkeys, and human volunteers. The major metabolites of S in the serum, urine, and feces of these species were determined by GC/MS and HPLC techniques. The metabolic pathways of S in these species involved reduction of the ketone group to an alcohol (S-OH), hydroxylation of the thiophene ring (T-OH), elimination of the thiophene ring to a dicarboxylic acid (S-COOH), and conjugation with glucuronic acid or taurine. In 72-hr urine and feces of these species after po dosing of 1.6 to 2 mg/kg of S, S and these metabolites accounted for 46 to 92% of the dose and were mainly excreted in the urine. S was present as a major product (excreted mainly in conjugated form) in all species. S-OH was a major component in guinea pig and dog but a minor one in other species. T-OH was identified as a major metabolite in monkey, rat, mouse, and man, but a minor one in guinea pig, and it was absent in the dog. S-COOH was present as the minor metabolite in mouse and rat, and present at trace levels in dog, monkey, and man. Conjugation of the propionic acid functionality with taurine was observed only in the dog; in the other species, conjugation with glucuronic acid was extensive. Absorption parameters of S in the rat and monkey were similar to those in man; however, other species were very different from man.

Animals↗