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Biomedical subjects

F Winkler

Publications and source records attributed to F Winkler.

At least 37 records · Page 2Linked to original sources
PubMed1998

[Early fetal development of the small intestine mucosa in cattle (Bos primigenius taurus)].

The development of the bovine small intestine was examined in 24 embryos and fetuses by light microscopic, scanning and transmission electron microscopic methods. Special reference was paid to the genesis of the epithelium and particularly of the villi intestinales. The primitive intestine consists of one layer of epithelial cells surrounded by mesenchym and tunica serosa. The fetal intestine (up to the 24th week of gestation) shows all the morphologic structures of the adult. In small intestine the development of cryptae and villi intestinales starts before the 7th week of gestation and progresses with a proximo-distal gradient. Epithelial proliferation that gives rise to primary epithelial villi makes epithelium become temporarily stratified. Finger-like secondary villi develop by proliferation of the mesenchym. In addition to this process mucosal folds occur in duodenum giving rise to villi by segmentation. At the same time the differentiation of epithelium starts. The fetal small intestine, like many other fetal tissues displays masses of glycogen.

Animals↗
PubMed1998

[Prenatal development of the lamina epithelialis in the phase of vacuolization of villous epithelial cells. Investigations on the intestines of cattle (Bos primigenius taurus L., 1758)].

The development of the bovine small intestine was examined in 39 embryos and fetuses by light microscopic and transmission electron microscopic methods. Special reference was paid to the histogenesis of the ephithelium. In contrast to the duodenum the epithelium of jejunum and ileum undergoes a degeneration by vacuolation of its villous epithelial cells. The demonstration of the acid phosphatase activity of these vacuoles shows their lysosomal character. This degenerative process of the small intestinal epithelium is also known in large intestine where it leads to the destruction of the intestinal villi. Both seem to be part of a 'principle of construction of the intestine of the vertebrates' (Wille, 1984).

Animals↗
PubMed1996

Digital pulse plethysmography as a non-invasive method for predicting drug-induced changes in left ventricular preload.

OBJECTIVE: Changes in the contour of the plethysmographically recorded digital pulse curve after nitrate ingestion are well known, but it has not been fully established whether these changes reflect nitrate action on left ventricular (LV) preload or afterload. Therefore, we compared the pulse wave contour after administration of equieffective doses of nitroglycerin and nifedipine. METHODS: In 20 patients with coronary artery disease we measured aortic blood pressure curve in the aorta ascendens, digital volume pulse curve with a photoelectric pulse pickup, Riva Rocci blood pressure and heart rate after administration of either 0.8 mg nitroglycerin or 10 mg nifedipine. RESULTS: Peak plasma concentrations of nitroglycerin and nifedipine were achieved 5 min and 20 min after ingestion of the drugs. Systolic aortic blood pressure decreased after both nitroglycerin and nifedipine to 19.4 mmHg, but diastolic blood pressure decreased only after nifedipine by 10.5 mmHg (P < 0.05). Riva Rocci blood pressures showed a similar time course. Heart rate increased from 67.4 to 70.9 beats.min-1 after nitroglycerin and from 58.9 to 69.4 beats.min-1 after nifedipine. The calculated a/b ratio of the aortic pressure curve increased after both medications (nitroglycerin, from 1.66 to 1.99; nifedipine, from 1.66 to 1.93) and its time course mimicked that of the systolic blood pressure. The a/b ratio of the digital pulse curve did not change after nifedipine, but showed a pronounced rise after nitroglycerin from 1.29 to 1.84. With regard to pharmacological actions, nitroglycerin causes a reduction in LV preload and afterload, whereas nifedipine has only LV-afterload-reducing activity. CONCLUSION: We conclude, that the reduction in afterload did not cause the typical changes in wave contour of the peripheral pulse curve which occur with organic nitrates. Most likely changes in the a/b ratio reflect changes in LV preload.

Adult↗
PubMed1995

Crystallization of the complex of human IFN-gamma and the extracellular domain of the IFN-gamma receptor.

A complex of human interferon-gamma (IFN-gamma) with the soluble extracellular domain of the IFN-gamma receptor alpha-chain (IFN-gamma-R) has been crystallised. Crystals of the complex were grown using PEG 4000 as the precipitating agent in the presence of beta-octyl glucoside. The receptor-ligand complex crystallizes in a monoclinic space group and diffracts to about 3.0 A resolution. Isomorphous crystals have been obtained with complex containing selenomethionine and cysteine mutants of IFN-gamma, which may facilitate the ongoing X-ray structure determination.

Amino Acid Sequence↗
PubMed1993

Topological similarities in TGF-beta 2, PDGF-BB and NGF define a superfamily of polypeptide growth factors.

BACKGROUND: The development of functional diversity through gene duplication and subsequent divergent evolution can give rise to proteins that have little or no sequence similarity, but retain similar topologies. RESULTS: The crystal structures of nerve growth factor, transforming growth factor-beta 2 and platelet-derived growth factor-BB show that all three are based on a cystine-knot plus beta-strands topology. There is very little sequence identity between the three proteins and the relationship between the structures had not been deduced from sequence comparisons. Each growth factor is usually active as a dimer; each exists as a dimer in the crystal, but the relative orientations of the protomers are different in each case. CONCLUSION: The structural motif of disulphide bonds and hydrogen-bonded beta-strands unexpectedly found in these three growth factors acts as a stable framework for elaboration of loops of low sequence similarity that contain the specificity for receptor interaction.

Amino Acid Sequence↗
PubMed1992

[Combination therapy with insulin and sulfonylurea compounds in type II diabetes mellitus--long-term results].

24 type II diabetic patients who displayed persistent fasting hyperglycaemia on maximal sulphonylurea therapy were treated with a combination of insulin and sulphonylurea agents. Satisfactory control of blood glucose levels was achieved by means of this therapeutic regimen. The good results on carbohydrate metabolism in these patients were maintained over a follow-up period of one year.

Aged↗
PubMed1991

Best for Britain?

Work in Oregon to develop a system for explicit rationing of healthcare has attracted worldwide attention. But, say Morton Warner and colleagues, it isn't a model the UK should follow.

Health Care Rationing↗
PubMed1991

Amino acid substitution (Ile194----Thr) in exon 5 of the lipoprotein lipase gene causes lipoprotein lipase deficiency in three unrelated probands. Support for a multicentric origin.

Studies on the molecular biology of lipoprotein lipase (LPL) deficiency have been facilitated by the availability of LPL gene probes and the recent characterization of gene mutations underlying human LPL deficiency. Typically, missense mutations have predominated and show a preferential localization to exons 4 and 5. This distribution supports earlier studies attributing functional significance to residues encoded by these exons. We now report a further missense mutation within exon 5 of the LPL gene in three unrelated patients. Amplification of individual exons by the polymerase chain reaction and direct sequencing revealed a T----C transition at codon 194 of the LPL cDNA which results in a substitution of threonine for isoleucine at this residue. The catalytic abnormality induced by this mutation was confirmed through in vitro mutagenesis studies in COS-1 cells. Transfection with a LPL cDNA containing the codon 194 transition resulted in the synthesis and secretion of a catalytically defective protein. The Thr194 substitution was associated with two different DNA haplotypes, consistent with a multicentric origin for this mutation.

Alleles↗
PubMed1990

Community health councils. Their role after the NHS White Paper.

Christine Hogg and Fedelma Winkler review the major and varied changes being introduced in the management of community health councils by different regional health authorities in the wake of the NHS Bill. This piecemeal approach may lead to more variations in the standards of service that individual CHCs offer the public and they argue that there needs to be a coherent policy about the role of CHCs and evaluation and monitoring of their functions.

Community Participation↗
PubMed1988

Competing for status.

Explore the source record for details and available documents.

Community Health Nursing↗
PubMed1987

[Psychogenic voice disorders. Contribution of clinical psychology].

Only a few psychological papers deal with functional dysphonia. Even fewer empirical studies are concerned with the importance of variations in personality and neurotic behavioural patterns that are typical of functional dysphonia. The enumeration of other functional disorders is important. We suggest that difficulties in self-confidence, social competence, and social fear as well as an increase in control of emotions contribute to the genesis of functional dysphonia. In order to reveal the psychological factors in the diagnosis of dysphonia, every phoniatric diagnosis must be supplemented by a clinical and psychological one.

Humans↗
PubMed1986

Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases.

We have synthesized several self-complementary oligodeoxynucleotides which contain bromodeoxyuridine in various positions within and outside of the recognition sequence for the EcoRI and EcoRV restriction endonucleases. These oligodeoxynucleotides are cleaved in the presence of Mg2+ by their respective enzyme. Upon irradiation by long-wavelength ultraviolet light and in the absence of Mg2+ they are cross-linked in low yield to their enzymes, forming 1:1 and 1:2 (oligodeoxynucleotide:enzyme subunit) adducts. Cross-linking occurs with both specific and non-specific complexes. With EcoRI the site of cross-linking was determined to be at or close to Met-137, i.e. in a region of the molecule implicated by other studies from our laboratory [Scholtissek et al. (1986) J. Biol. Chem. 261, 2228-2234] in the binding and cleavage of the substrate.

Base Sequence↗
PubMed1986

[Cardiac rehabilitation of elderly patients with multiple diseases].

The cardiac diseases present in 274 multimorbid, older patients (mean age 72,42 yrs.) have been examined in the study reported. Careful differentiation of cardiac diagnoses present at the time of admission was found to be of utmost importance in outcome prediction. The combination of cardiac decompensation and arrhythmia, and recurrent infarction in the presence of myocardial scarring, respectively, had been the most important factors concerning functional capacity at time of discharge. Mortality, too, was found to be related to these criteria.

Aged↗
PubMed1983

A monoclonal antibody to the heavy chain of clathrin.

Monoclonal antibodies have been raised to pig brain triskelions and one clone, DC41, was found to recognize the clathrin heavy chain by immunoblotting. However, both by immunofluorescence and immunoelectron microscopy, and in complete contrast to polyclonal anti-clathrin antibodies, monoclonal DC41 did not label either coated pits or coated vesicles anywhere in the cell. Instead it appeared to label the cell cytoplasm. These data suggest that DC41 recognizes a cytoplasmic form of clathrin, perhaps that form produced by uncoating of coated vesicles which is then ready to re-build another coated pit.

Animals↗