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Biomedical subjects

F Vogel

Publications and source records attributed to F Vogel.

At least 91 records · Page 5Linked to original sources
PubMed1989

A mammary-derived growth inhibitor (MDGI) related 70 kDa antigen identified in nuclei of mammary epithelial cells.

The aim of the present study was to investigate the expression of the mammary-derived growth inhibitor (MDGI) and the subcellular localization of MDGI-related antigens in bovine mammary glands. Cell-free translation of poly(A+) = RNA, immunoprecipitation with rabbit anti-MDGI-antibodies, and estimation of the relative contents of MDGI by a radioimmunoassay in mammary tissue of different functional states revealed that the 13 kDa MDGI was dramatically increased in terminally differentiated mammary tissue compared with the proliferating tissue from pregnant animals. To address the question of tissue localization, polyclonal anti-MDGI antibodies and antibodies directed against a synthetic peptide corresponding to residues 69 to 78 of MDGI were used. Western blotting of tissue fractions revealed the cytosolic and microsomal localization of MDGI. Additionally, both types of antibodies detected a 70-kDa antigen in the nuclear fraction of differentiated mammary glands. Salt extraction and DNase I digestion of isolated nuclei, as well as chromatin purification, indicated an association of the 70-kDa antigen with the chromatin. By means of the immunogold technique, MDGI-related antigens were localized within euchromatic nuclear regions of epithelial cells in the intact differentiated mammary gland. The immunostaining was markedly diminished in the proliferating tissue. This finding raises the possibility that MDGI and the 70-kDa antigen influence cell proliferation by acting on gene expression within the nuclei of mammary glands.

Animals↗
PubMed1989

The problem of our common mitochondrial mother.

It has been suggested that the mitochondrial DNA (mtDNA) of all present-day human beings stems exclusively from one woman who lived about 200000 years ago in Africa; examination of the problem by the mathematical theory of random walks supposedly renders alternatives very unlikely. However, a statistical argument first used by Fisher indicates that this hypothesis is untenable, at least if the assumptions made by previous workers are accepted. All present-day mtDNA might go back to one individual, especially if small populations and population bottlenecks with very small numbers of reproducing individuals are assumed; nevertheless, this phase in the evolution of Homo sapiens probably dates back considerably more than 200000 years.

Biological Evolution↗
PubMed1989

A genetic and anthropological study of atlanto-occipital fusion.

Families of 20 probands with atlanto-occipital fusion were studied, and the neurological complications in these patients described. In X-ray studies of 115 close relatives, 4 additional cases (3.5%) with the same anomaly were detected. In a comparison of adult patients with closely related age- and sex-matched controls, all anthropological measurements except length and breadth of the head tended to be smaller in the patients; for height, weight, leg and foot length, and the robusticity index, these differences were statistically significant.

Adolescent↗
PubMed1989

Management of lymphocysts after radical gynecologic surgery.

Pelvic lymphocysts developed in 3 of 124 patients undergoing radical surgery for cervical cancer. All were managed by percutaneous aspiration under local anesthesia in an outpatient setting. Sclerosis was required in one patient. This management plan is superior to laparotomy in being less morbid and equally effective.

Adenocarcinoma↗
PubMed1989

Impact of complex genetic conditions on public health.

Complex genetic diseases are often common: in most common diseases liability is influenced by genetic variation. The ways in which this variation is analyzed are discussed using diabetes, affective disorders and schizophrenia as examples. Molecular biology has opened new paths for a more incisive analysis of genetic heterogeneity and biological mechanisms. However, important population genetic aspects such as mutation and selection are largely unexplored. Therefore, predictions regarding possible increases or decreases in frequencies of susceptibility genes are hardly feasible. In many cases, however, the public health impact of such diseases can be alleviated and the quality of life of the individual can be improved, by appropriate adaptation of relevant environmental factors.

Diabetes Mellitus↗
PubMed1989

[Arrhythmogenic effect of flecainide--treatment with i.v. magnesium].

We report on a 46-year-old woman with ventricular tachycardia and ventricular fibrillation after starting antiarrhythmic drug therapy with flecainide. The flecainide acetate plasma levels were always in the normal range. A successful therapeutical intervention was induced with two i.v. applications of 1,000 mg magnesium glutamate. A persistent suppression of ventricular rhythm disturbance was accomplished by continuous i.v. application of 4 mg magnesium glutamate/min.

Electrocardiography↗
PubMed1989

[The effectiveness of intratracheal antibiotic administration. Clinical, microbiologic and pharmacologic results].

In 199 artificially ventilated patients of an internal intensive care unit clinical, bacteriological and pharmacological effects of endotracheally administered gentamicin were investigated. The dose schedule was 2-4 x 40 mg gentamicin/day. The incidence of secondary achieved pneumonia was reduced from 70% to 18%. The endotracheal colonization of pathogenic microorganisms reached 29.6% concerning bacterial microorganisms and 61% concerning fungi, 51.8% of all specimen were sterile. During 4 years of investigation there were found 19 secondary resistances of different bacteria, 12 persisted. Serum concentrations of gentamicin under endotracheal administration of 40 mg in 6-h-intervals didn't reach therapeutic values. In case of renal disorder the dose interval should be prolonged to twelve hours.

Adult↗
PubMed1988

Condensation of vector DNA by the chromosomal protein HMG1 results in efficient transfection.

The aim of this study was the search for a method of vector packaging using natural chromatin constituents. The interaction of the chromosomal non-histone protein HMG1 with a vector plasmid (pLTEneo) was studied by sedimentation analysis and electron microscopy at physiological salt concentration. At high protein input the complexes exist in a condensed, monodisperse form sedimenting with 80 S irrespective of the supercoiled or relaxed conformation of DNA. Saturation binding is already observed at much lower input ratios. Dilution of 80 S complexes results in decondensation of the complexes. In the decondensed complex form, HMG1 binds in a bead-like manner to specific DNA regions. Condensation by HMG1 is sufficient to introduce the vector into mammalian cells without the need for unphysiological additives. The transfection rates were similar to or even higher than those obtained by the calcium phosphate coprecipitation technique.

Animals↗
PubMed1988

Analysis of hamster lymphomas for the presence of hamster papovavirus DNA.

The hamster papovavirus (HaPV) is a polyomavirus isolated from skin epitheliomas arising spontaneously in young Syrian hamsters. It can induce lymphomas and leukaemias in newborn hamsters. Although no virus particles are detectable by electron microscopy, high amounts of monomeric and oligomeric forms of extrachromosomal HaPV DNA molecules are found in the lymphoma cells. These molecules display deletions of about 300 nucleotides in length. Their role in the lymphoma induction is discussed.

Animals↗
PubMed1987

Genetic counseling in the epilepsies. I. Genetic risks.

Rules are proposed for genetic counseling in the epilepsies. In a first section, some attempts at subdividing epilepsies by dichotomous classifications are discussed critically. Then, the typical EEG patterns found in epileptic diseases as well as their genetic bases are described. The third section comprises various clearly defined and sufficiently well-described epileptic syndromes. In a fourth section, a kind of "record sheet" is suggested to help the genetic counselor in planning his procedure. Throughout this review, we have stressed that genetic counseling in the epilepsies should not be confined to risk assessment but should include considerations on course of the disease, prognosis, and chances of therapy.

Electroencephalography↗
PubMed1987

A monozygotic twin pair with Rett syndrome.

A five-year-old, monozygotic, Turkish female twin pair with Rett syndrome is described. The twins are almost completely concordant in all clinical signs. This observation suggests a genetic cause of Rett syndrome.

Child, Preschool↗