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Biomedical subjects

F Tanaka

Publications and source records attributed to F Tanaka.

At least 289 records · Page 16Linked to original sources
PubMed1981

Diagnostic procedures for assessment of disease extent in prostatic carcinoma.

Staging of carcinoma of the prostate was performed on patients using Watanabe's method of transrectal ultrasonotomography (N = 37), pedal lymphography (N = 10), and bone scanning with technetium phosphate (N = 35). Of 14 patients found to have tumor confined within the prostate following routine examination, 4 were elevated to a higher disease stage after sonographic evaluation. Thirty-two percent of 25 patients with normal bone X-rays had abnormal bone scans. Four cases with positive node metastases on lymphogram had already presented with skeletal involvement. The values of these techniques in the assessment of disease extent in prostatic carcinoma are compared and briefly discussed.

Bone Neoplasms↗
PubMed1981

Mortality and survival for Down syndrome in Japan.

Mortality and survival data for 1,052 Japanese patients with Down syndrome who were born between 1966 and 1975 were analyzed. The survival rate at age 10 was estimated to be about 86%. Mortality in each age group for Down syndrome was elevated over that of the general population. In the survival rate at age 10, there was no significant difference between males and females, but the difference between cases with and without congenital heart disease was highly significant. Using data from this study-for mortality up to age 10-and from the study of institutionalized cases for mortality over age 10, a hypothetical life table was constructed; it shows that the life expectancy at birth for cases with Down syndrome is nearly 50 years.

Adolescent↗
PubMed1980

Pulse fluorimetry of N-(1-pyrenesulfonyl)dipalmitoyl-L-alpha-phosphatidylethanolamine in concanavalin A-stimulated human lymphocytes.

Human peripheral lymphocytes were cultured with a fluorescent probe, N-(1-pyrenesulfonyl)dipalmitoyl-L-alpha-phosphatidylethanolamine, and with concanavalin A. Fluorescence microscopic observations revealed that in lymphoblasts, pyrenesulfonyl dye was distributed mainly in vacuoles whereas in normal cells cultured without concanavalin A the dye was distributed exclusively in plasma membranes. The fluorescence spectra of the pyrenesulfonyl group incorporated into the cells exhibited two emission maxima, band A (monomer fluorescence of the pyrenesulfonyl group at about 400 nm) and band B (dimer fluorescence of the dye at about 500 nm). The values of the fluorescence lifetime measured at bands A and B indicated that in the absence of concanavalin A, the environment surrounding the pyrenesulfonyl group at the lipid/water interface became more hydrophilic with cultivation time. Concanavalin A made the environment of the interface more hydrophobic than that of lymphocytes cultured without concanavalin A. Fluorescence polarization measured at band A revealed that the mobility of pyrenesulfonyl monomers at the aqueous interface of the membranes was reduced upon concanavalin A stimulation.

Cell Membrane↗
PubMed1980

Picosecond fluorescence lifetime of the coenzyme of D-amino acid oxidase.

Conformational difference surrounding the coenzyme, FAD, of D-amino acid oxidase (D-amino-acid:O2 oxidoreductase (deaminating), EC 1.4.3.3) between its monomeric and dimeric forms were examined by observing fluorescence of FAD. The fluorescence lifetimes of the coenzyme was measured directly with a mode-locked Nd:YAG laser and a streak camera in picosecond region. The values of lifetime of FAD fluorescence in the monomer and dimer were 130 +/- 20 ps and 40 +/- 10 ps, respectively. The relative quantum yield of the fluorescence of FAD combined with the protein to that of free FAD depended on the concentration of the enzyme; it was higher at lower concentration. Comparing the lifetime with relative quantum yield of FAD combined with the protein, it is concluded that the fluorescence is quenched mostly by a dynamic process. These results indicate that the distance between the isoalloxazine nucleus and a quencher is nearer in the dimer than in the monomer.

D-Amino-Acid Oxidase↗
PubMed1980

Analysis of sera from patients with breast cancer for blocking and induction of leukocyte adherence inhibition.

The correlation of serum capacity for blocking and arming with effector cell reactivity has been examined in patients with breast cancer by using the leukocyte adherence inhibition (LAI) assay. It was found that sera from breast cancer patients possessed the ability to make nonimmune mononuclear cells specifically reactive (i.e., armed) to the breast cancer extract and to specifically block the reactivity of mononuclear cells from breast cancer patients with the cancer extract. Sera from patients with breast cancer whose mononuclear cells were nonreactive to the corresponding tumor extract (6/8) showed higher blocking than sera from patients whose cells showed reactivity to the corresponding tumor extract (3/13). Similarly, sera from patients with positive LAI response (6/13) induced arming of normal mononuclear cells more often than sera from patients with negative LAI (2/8). There also appeared to be an inverse correlation between the capacities of serum from breast cancer patients to exhibit blocking and arming. That is, serum arming activity was found only in sera which showed no blocking, and serum blocking activity was detected only in sera without arming, activity, except for one sample. The possibility is discussed that immune complexes play a key role in both serum activities, and that their biphasic character might be dependent on the ratio of antigen of antibody.

Antigen-Antibody Complex↗
PubMed1980

Enzyme immunoassay of hepatitis B surface antigen with use of the "Hepanostika" test kit and a new photometer, "Photo-Elisa 1".

We describe an enzyme immunoassay for hepatitis B surface antigen, with use of a Hepanostika test kit and "Photo-Elisa 1," a photometer capable of measuring small quantities of test material. Because the length of the optical path in the "Photo-Elisa 1" is only 1.2 mm, volumes as small as 15 micro L can be can be measured. Accuracy is enhanced through cancellation of background absorbance by measurement at two wavelengths. Results obtained with the kit compare well with those by radioimmunoassay. By substituting photometric reading for visual reading, Hepanostika provides a more nearly accurate screening test.

Enzyme-Linked Immunosorbent Assay↗
PubMed1979

The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane.

A new procedure is established for the analysis of alkaline phosphatase isoenzymes. The electrophoretic separation on cellulose acetate membrane coupled with the detection of alkaline phosphatase activity with 4-methyl-umbelliferyl phosphate as a substrate is described. The proposed method would be useful for the analysis of sample of micro-scale quantities and low activities.

Alkaline Phosphatase↗