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Biomedical subjects

F Sundler

Publications and source records attributed to F Sundler.

At least 487 records · Page 27Linked to original sources
PubMed1979

Duodenal endocrine cells in adult coeliac disease.

Using immunohistochemical techniques we studied duodenal biopsies from 18 patients with coeliac disease and 24 patients with normal duodenal morphology. We had access to antisera against the following gastrointestinal peptides: cholecystokinin (CCK), gastric inhibitory peptide (GIP), gastrin-17, glucagon-enteroglucagon, motilin, neurotensin, pancreatic peptide (PP), secretin, somatostatin, substance P and vasoactive intestinal peptide (VIP). The somatostatin, GIP, CCK, and glucagon cells were increased in number in coeliac disease. The number of motilin cells was slightly increased, while secretin cells were reduced. Cells storing gastrin-17, substance P, or neurotensin were rare in all patients regardless of diagnosis. No PP immunoreactive cells were found and VIP was localised to neurons only. In biopsies from patients having a mucosa with ridging of villi the number of the various endocrine cell types did not differ from that in the control group.

Adult↗
PubMed1979

Vasoactive intestinal polypeptide nerves in human upper respiratory tract.

Immunoreactive vasoactive intestinal polypeptide (VIP), a recently recognized neuropeptide with a possible transmitter function has been demonstrated in nerves of the human upper respiratory tract. VIP nerves predominate in the inferior turbinate of the nose and in the tracheal wall. They are distributed in the subepithelial connective tissue, around blood vessels and seromucous glands. Scattered VIP nerves are observed also in the tracheal smooth muscle. VIP immunoreactive nerve cell bodies are found only in the tracheal wall, suggesting a local origin of the VIP nerves in this organ. Available information indicates that the VIP-containing nerves constitute a separate component of the autonomic nervous system in addition to the adrenergic and cholinergic ones.

Gastrointestinal Hormones↗
PubMed1979

On the immunocytochemical localization of the vasoactive intestinal polypeptide.

The distribution of vasoactive intestinal polypeptide (VIP) immunoreactive nerves and endocrine cells in the gastrointestinal tract and pancreas of a number of mammalian and submammalian species has been examined in order to throw light on the exact localization of this peptide. Seven out of 8 VIP antisera demonstrated numerous nerve fibers in the gut, whereas one antiserum (TR2) revealed only scattered, few nerve fibers. The distribution of endocrine cells demonstrated by the different VIP antisera varied considerably. Thus, some antisera demonstrated only endocrine cells in the feline antrum, others only colonic endocrine cells and still others only endocrine cells of the upper gut and pancreas. The variability in staining pattern of endocrine cells as well as recent radioimmunological data makes it opportune to suggest that true VIP is a neuronal peptide and that endocrine cells store peptides resembling, but not being identical with, VIP (VIPoids).

Animals↗
PubMed1979

Presence and influence of cholinergic nerves in the mouse thyroid.

The presence and influence of cholinergic nerves in the mouse thyroid was studied by histochemistry and measurements of changes in blood radioiodine (BRI) levels. Numerous nerve fibers displaying specific acetyl choline esterase activity were found, not only as a dense network around vessesl but also as single fibers running around and between thyroid follicles. In stress-adapted normal mice, injection of carbamyl choline (CCh) reduced the BRI levels. In mice whose TSH secretion was suppressed by L-T4, neither CCh nor atropine had any measurable influence on the BRI levels when given alone. However, CCh pretreatment reduced and atropine pretreatment enhanced the TSH-induced BRI increase in such animals. It is concluded that the murine thyroid contains numerous cholinergic nerves that may influence not only thyroid blood flow but also thyroid hormone secretion directly. This direct influence appears to be an inhibitory one, mediated via muscarinic receptors in the follicle cells.

Animals↗
PubMed1979

Majority and minority cell populations in GEP and bronchial endocrine tumours.

Gastro-entero-pancreatic (GEP) and bronchial endocrine tumours have been studied by immunohistochemistry using specific antisera against a variety of hormonal and neuronal peptides. In gastrinomas numerous tumour cells were found to contain GH-like immunoreactivity. These cells were identical with those storing gastrin. Gastrinomas as a rule were extremely heterogeneous containing a variety of minority cell populations, including CCK immunoreactive cells and neurotensin immunoreactive cells. Glucagonoma cells were found to store GIP-like material in addition to glucagon. In some insulinomas calcitonin-like material was encountered in the insulin producing tumour cells. In both glucagonomas and insulinomas other pancreatic endocrine cell types constituted minority cell populations. One intestinal somatostatinoma contained gastrin cells as a minority cell population. Bronchial endocrine tumours contained scattered cells displaying ACTH-like or enkephalin-like immunoreactivity. Two such tumours in addition contained cells displaying neurophysin immunoreactivity.

Adenoma, Islet Cell↗
PubMed1979

Variated serum gastrin concentration: trophic effects on the gastrointestinal tract of the rat.

Gastrin is thought to be a trophic agent for the digestive tract and the pancreas. This concept was studied on rats subjected to various operations designed to create hyper- or hypo-gastrinemia. Elevated serum gastrin concentration resulted from vagal denervation, antrum exclusion and fundectomy. Reduced serum gastrin concentration resulted from antrectomy. Antrum exclusion but not vagotomy increased the weight and height of the oxyntic mucosa; antrectomy had the opposite effects. There were no trophic changes in the small and large bowel or pancreas, attributable to the serum gastrin concentration. In the bypassed duodenum (following antrum exclusion or antrectomy B II) the villus height was greatly lowered suggesting that in this location the passage of food acts as a trophic stimulant. Our results show that endogenous gastrin exerts a trophic effect on the oxyntic mucosa; there was no evidence for a similar effect on the extragastric gut and pancreas.

Animals↗
PubMed1979

Spontaneous argyrophil cell carcinoid in the glandular stomach: immunohistochemical study of gastric endocrine cells in normal and tumour-bearing mastomys.

Endocrine tumours (argyrophil cell carcinoids) are frequent in the oxyntic mucosa of mastomys. The tumour is notable for its high histamine content and for its high histidine decarboxylase activity. The tumour is thought to arise from the histamine-storing, enterochromaffin-like cells of the oxyntic mucosa. They are of two ultrastructurally distinguishable types, ECL cells and A-like cells, both of which have been demonstrated in the tumour. Identical cells have been demonstrated in the oxyntic mucosa of the rat; there is much evidence that in this species the functional activity and the number of these cells are determined by the serum gastrin concentration. However, tumours have never been found to arise from these cells in the rat. As an initial step in an attempt to explain the formation of the gastric endocrine tumour in the mastomys we examined the distribution and frequency of occurrence of endocrine cells in the mastomys stomach. Gastrin cells in the antrum of mastomys seemed to occur in about the same frequency as in the antrum of rat and mouse. 5-HT-storing enterochromaffin cells, however, were considerably more numerous in the mastomys, whereas the somatostatin cells in the antrum were fewer. The number of enterochromaffin-like cells and somatostratin cells in the oxyntic mucosa of mastomys was much lower than in the rat and mouse. Once developed, the gastric endocrine tumour seems to reduce the antral gastrin cell number; the larger the tumour the greater the reduction.

Animals↗
PubMed1978

Is GIP a glucagon cell constituent?

"Gastric inhibitory peptide" or "glucose-dependent insulin-releasing peptide" (GIP) is a member of the gut hormone family. Its physiological action is thought to be related to its insulinotrophic effect. The occurrence and distribution of GIP was studied by immunohistochemistry. In all species examined including man, GIP immunoreactivity was found to reside in the glucagon cells of the pancrease and gut. Three pancreatic glucagonomas were found to contain numerous cells displaying GIP and glucagon immunoreactivity. The GIP antiserum used did not cross react with either pancreatic-type or gut-type glucagon (GLI).

Animals↗
PubMed1978

Immunoreactive neurotensin and somatostatin in the chicken thymus. A chemical and histochemical study.

Two distinct populations of endocrine cells in the chicken thymus display neurotensin and somatostatin immunoreactivity, respectively. Both cell types are few in number at hatching but proliferate rapidly during the first week. The neurotensin cells are 'Grimelius-positive' and 'Hellerström-Hellman-negative'. The somatostatin cells are 'Grimelius-negative' and 'Hellerström-Hellman-positive'. Both cell populations are non-argentaffin. The somatostatin-like material extracted from chicken thymus behaves immunochemically and chromatographically similar to synthetic ovine somatostatin, while the neurotensin-like material, from the thymus as well as from the gut, differs from synthetic bovine neurotensin in that it appears larger in size and more basic.

Animals↗
PubMed1978

Leu-enkephalin-like material in nerves and enterochromaffin cells in the gut. An immunohistochemical study.

The distribution and cellular localization of leu-enkephalin in the gut and pancreas was studied by immunohistochemistry using two different antisera, one specifically directed against leu-enkephalin and the other cross reacting with met-enkephalin. The results were identical with both antisera. In all species examined, enkephalin-immunoreactive material was found in nerves of the smooth muscle, particularly numerous in the myenteric plexus. Here, immunoreactive nerve cell bodies were observed occasionally. In addition, enkephalin-immunoreactive material was demonstrated in gut endocrine cells of chicken, mouse, rat, pig and monkey but not of guinea pig, cat and man. Enkephalin cells were detected also in the exocrine parenchyma of the porcine pancreas. They were rare in the gut of mouse, rat and monkey but numerous in the antrum and duodenum of pig where they were identified as 5-hydroxytryptamine-storing enterochromaffin cells. The enkephalin-containing cells of the porcine antrum and duodenum were defined ultrastructurally by the consecutive semithin/ultrathin section technique. The ultrastructural features were typical of enterochromaffin cells, the most characteristic ones being the irregular shape and high electron density of the cytoplasmic granules. The immunoreactive material was confined to the cytoplasmic granules.

Animals↗
PubMed1978

Fluorescence histochemistry of peptide hormone-producing cells: observations on the phenanthrenequinone method for the demonstration of arginine residues.

Phenanthrenequinone is a sensitive and specific fluorescence histochemical reagent for monosubstituted guanidines. It is probable that it selectively demonstrates the guanidino group of arginine residues of proteins and peptides. Phenanthrenequinone induces moderate to intense fluorescence in gastric chief cells, parenchymal cells of the pancreas, and certain peptide hormone-producing cell types such as the GH cells of the adenohypophysis and the glucagon cells of the pancreatic islets. Similar fluorescence spectra were obtained from an arginine-containing peptide in a histochemical model and from the GH cells of the adenohypophysis following exposure to phenanthrenequinone. We conclude that the cells demonstrated with this reagent store peptides or proteins rich in arginine.

Adrenal Glands↗
PubMed1978

Jejunal endocrine tumor composed of somatostatin and gastrin cells and associated with duodenal ulcer disease.

A case of malignant endocrine tumour of the jejunum, associated with severe duodenal ulcer is described. The tumour and a local metastasis were examined by immunohistochemistry and found to contain abundant somatostatin-immunoreactive cells together with less numerous cells displaying gastrin immunoreactivity. This is to our knowledge the first case of intestinal somatostatinoma. The presence of gastrin cells in the tumour may explain the ulcer diathesis.

Duodenal Ulcer↗