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Biomedical subjects

F Streiff

Publications and source records attributed to F Streiff.

At least 37 records · Page 2Linked to original sources
PubMed1989

Amodiaquine-induced agranulocytosis: report of a case with in vitro studies of granulocyte-macrophage progenitor cells.

We report a case of amodiaquine-induced agranulocytosis in a 60-year-old woman. Four months after the agranulocytosis episode we investigated the effect of the drug using in vitro agar culture techniques. Amodiaquine at increasing concentrations (0.005, 0.05 and 0.5 microgram/ml) displayed an inhibitory effect, probably dose-dependent, on the growth of the patient's bone marrow GM-CFU colonies in the absence of autologous serum. In contrast, no effect was found on the colony and cluster growth of bone marrow samples from 13 healthy controls. Though it has been shown in several cases that amodiaquine-induced agranulocytosis occurs via immune-mediated mechanisms, our data are in support of a direct toxic effect of the drug on abnormally sensitive myeloid progenitor cells.

Agranulocytosis↗
PubMed1989

Analysis of blood CD4+ and CD8+ T-lymphocyte subsets with monoclonal antibodies. Comparison of the Genetic Systems CD4/CD antigen typing kit with conventional indirect immunofluorescence microscopy and the automated Technicon-H1 Enzyme Immunoassay (EIA).

The Genetic Systems Technique (GS: direct immunofluorescence microscopy with ethidium bromide counterstaining of nuclei) was tested for quantitative analysis of T-lymphocyte subsets in human peripheral blood. The monoclonal antibodies anti-CD4 and anti-CD8 were used for detection of T-helper and T-suppressor cells respectively and the results compared to those obtained by conventional indirect immunofluorescence microscopy and the Technicon Enzyme Immunoassay (EIA) with automated reading. The GS technique provided results correlating well with both indirect immunofluorescence and EIA techniques. Moreover, this method has two advantages: it is less time-consuming than the indirect immunofluorescence microscopy and necessitates less expensive and more commonly available equipment than the automated EIA technique.

Antibodies, Monoclonal↗
PubMed1988

Use of a new plasma separation membrane for plasma donation. Technical and biological results.

Donor safety and high-quality plasma are important assumptions for a successful plasma donation. The manual technique has two major inconveniences: It is time consuming and there are risks in the transfusion because the system is not closed. Among the available technological options, filtration has great future prospects insofar as a hemocompatible and efficient membrane can be made available and at low cost. Hemascience Travenol developed the polycarbonate membrane for the first disposable. Because of a high rate of no or very low plasma flow (5-10%), the company equipped the disposable with a new membrane: the nylon membrane. This membrane was tested according to biological and technical protocols; 39 disposables were used. The anticoagulant was acid citrate dextrose formula A. The results were good: time to collect 600 g of plasma, 34 min (only two occurrences of low flow); no problem with blocking or clotting. The main biological data show no problem with molecule activation (complement factors, coagulation factors), a good recovery of Factor VIII, and no contamination of platelets (5 +/- 2 X 10(3)/ml). No side-effects in the donor were noted. These results confirm that the Hemascience Travenol system is one of the best automatic plasmapheresis systems and could be used to separate great quantities of plasma from donors.

Filtration↗
PubMed1988

[Evaluation of an automated system of measuring erythrocyte aggregation in dysglobulinemia treated by plasma exchange].

Characteristics of a new erythrocyte aggregameter were evaluated in 7 patients with monoclonal dysglobulinemia (5 multiple myeloma, 1 Waldenström disease, 1 CLL with monoclonal immunoglobulin) treated by plasma exchange. This apparatus measures modifications of light retro-diffused by erythrocytes suspension after shear arrest. All parameters measured: Ta, Tf, S10, gamma D, gamma S were modified after plasma exchange: either immediately after the first or after the second or third plasma exchange. These findings demonstrate the value of this new technique for evaluating red cell aggregation. These data should be completed by rheological assessment of dysglobulinemia treated by plasma exchange.

Automation↗
PubMed1988

[Erythropheresis in 4 cases of congenital hemolytic anemia].

Erythrocytapheresis was performed 6 times in 4 patients with sickle cell anemia and thalassemia. Good results were obtained, particularly in pregnant patients, no crisis being observed during pregnancy. In the other patients the painful crisis was suppressed over 4 months. The technic is very easy. The Haemonetics H 30 was used, 70% of red cell volume being removed and replaced with leukocyte-poor blood.

Adolescent↗
PubMed1988

[Treatment of systemic scleroderma using plasma exchange. A study of 19 cases].

Generalized scleroderma (GS) is associated with dysimmunity anomalies suggesting possible benefits of plasma exchange (PE) therapy. Nineteen patients with GS were treated by PE (volume of plasma exchange equivalent to 5-6% body weight and replacement by 4% human albumin), initially three times weekly, then weekly, bi-monthly and monthly (total duration 12-18 months). Clinical and paraclinical follow up was for an average of more than 2 years after the end of PE (mean number 17 per patient). Clinical results were assessed as positive and lasting in 11 cases (57.9%), two cases remaining stable and three cases worsening (one death from heart failure). The remaining three cases were failures in application of treatment (difficult venous approach). Improvement was noted in cutaneous sclerosis (62% of cases), trophic disorders (recovery in 6 of 7 cases) and articular manifestations. Vasomotor disorders were improved in only 20% of cases and visceral lesions unaltered. Results of capillaroscopy showed improvement in 5 of 11 cases. Biological values could not be correlated with either the course or the therapeutic efficacy. General tolerance to PE was good but the venous approach must be of good quality. These findings suggest the need for a randomized trial to define the place of PE in the treatment of GS.

Adult↗
PubMed1988

[Pyoderma gangrenosum: treatment with plasma exchange (4 cases)].

Four patients with pyoderma gangrenosum were treated by plasma exchange. The series included one woman (cervical localization) and three men (sural localization in 1 case, multiple trunk and facial localizations in the second case and multiple, recurrent localizations on trunk and limbs in the third case). In 2 cases another disease was associated (ulcerative colitis in 1 case and Crohn's disease in the other). Between 6 and 12 plasma exchange sessions were carried out in combination with corticotherapy (1/2 mg/kg/day). Results in 2 cases were rated as very good: progression of the disease interrupted and no further pain after the 1st plasma exchange, rapid healing of lesions (a skin graft was necessary for a lesion of hand exposing tendons). A good result was obtained in the 3rd case with interruption of progression of lesions after the 1st plasma exchange but slower relief of pain. Treatment was considered a failure in the 4th case since there was no obvious regression in lesions or pain. Three of the 4 patients had no recurrences, one patient developing recurrences on 4 occasions responding well to plasma exchange each time. Pyoderma gangrenosum is a good indication for use of plasma exchange, which limits extension of ulcerating lesions, suppresses or reduces pain and decreases esthetic prejudice.

Adolescent↗
PubMed1988

[Treatment using plasma exchange of massive acute hemolysis. 15 cases].

Fifteen patients admitted to an intensive care unit for massive acute hemolysis (MAH), defined by a free hemoglobinemia of more than 60 mumol/l, were treated by plasma exchange (PE) after a mean period following onset of 48.5 39 hours. The aim of plasma exchange therapy was to obtain early purification of hemoglobin and other substances released by red cell lysis and to prevent serious complications of MAH such as shock and RVHF or acute renal failure. Treatment by PE produced a decrease in initial hemoglobinemia of 76%, this level then remaining stable. The PE also appeared effective in the prevention and treatment of shock and RVHF, but much less so for installed acute renal failure. Comparison of results with those of a previous series of 16 patients with MAH treated by exsanguino-transfusion showed that PE was more effective, simpler to perform and less aggressive for early treatment of MAH, if rigorous conditions are applied.

Adult↗
PubMed1988

[Evaluation of cascade filtration on a test sample].

Double filtration or filtration in series can be consider as an effective means of removing proteins such as IgG, IgM, immune complexes, lipoproteins from plasma. In this study, we evaluated, using a biological and technical protocol: Kuraray (2A and 4A) and Dideco (Albusave) filters. Results were good by analysis of the sieving coefficient, but the method had 2 inconveniences: problem of slogging of the columns after filtering 2 liters and too high a reject coefficient of albumin. No effective solutions exist to avoid these two problems. Nevertheless, the filtration in series technic seems to be a good method for removing cholesterol and we have undertaken new studies to improve this system.

Blood Component Removal↗
PubMed1988

Lymphocyte proliferative responses in haemophiliac patients: relations to clinical and immunological findings.

Blood lymphocyte proliferative responses to mitogens were studied in 65 patients with haemophilia (haemophilia A: 54 patients, haemophilia B: 11 patients) in parallel with 39 male control subjects. As a group, patients with haemophilia did not demonstrate abnormal proliferative responses to phytohaemagglutinin (PHA), Concanavalin A (ConA) and pokeweed mitogen (PWM) when compared with healthy controls. When the patients were analysed according to their seropositivity for antibody to human immunodeficiency virus (HIV), those who were positive had significantly decreased PHA, ConA and PWM responses. Haemophiliac patients with T4+/T8+ ratios less than 1 had reduced proliferative responses to PHA, ConA and PWM when compared to patients with ratios greater than 1. No significant difference in mitogen responses were found when the patients were analysed according to the presence or absence of palpable lymphadenopathy. Those patients with haemophilia A who had received more than 5 x 10(4) units of factor VIII during the two years preceding the study showed no significant difference in PHA, ConA and PWM responses when compared to patients receiving less.

Adolescent↗
PubMed1987

Lack of linkage between HLA and multiple endocrine neoplasia type 2 in a French family.

In this study, linkage between HLA and a dominant gene determining multiple endocrine neoplasia type 2 (MEN 2) in a large pedigree was investigated. All lod scores for recombination fractions ranging from 0 to 0.45 were negative. If we pool data from our family and the families studied by Jackson et al. (1976) and Simpson & Falk (1982), a link between HLA and the locus for MEN 2 can be excluded. Linkage studies with various markers and pooled data should be pursued to permit detection of high risk individuals and to identify a genetic defect.

Genetic Linkage↗
PubMed1987

[Clinical and biological evaluation of the preventive role of anti-cytomegalovirus specific immunoglobulins in bone marrow grafts. Randomized study of 60 patients].

The effects of i.v. cytomegalovirus (CMV) immunoglobulin given for prophylaxis of CMV infections in recipients of allogeneic and autologous marrow transplants were evaluated in a randomized trial: 60 patients were randomly assigned to receive (30 patients) or not to receive (30 patients) CMV immunoglobulin for a period of 90 days after transplantation. As to the allografted patients, the cumulative incidence of asymptomatic and symptomatic CMV infections was significantly reduced in the CMV immunoglobulin-treated group as compared to the control group (56.5% versus 92.9%, P less than 0.05). No other statistically significant effect of CMV immunoglobulin could be found. In particular, the incidence of symptomatic CMV infections (including interstitial pneumonia), the mean delay of post-transplant viraemia and haematopoietic recovery were similar in the control and CMV immunoglobulin-treated groups. We conclude that prophylactic CMV immunoglobulin administration, as designed in our study, is no more than marginally effective and cannot be recommended without additional trials.

Antibodies, Viral↗
PubMed1986

[Occurrence of non-A, non-B post-transfusion hepatitis in heart surgery. Prospective study on the value of the determination of serum alanine aminotransferase and detection of anti-HBc antibodies in blood donors].

We studied a group of 64 patients undergoing cardiac surgery for the occurrence of post-transfusion hepatitis during a follow-up period of 5 months. They received blood units (packed red cells in saline-adenine-glucose medium and/or fresh frozen plasma exclusively) from 447 volunteer donors. Post-transfusion hepatitis was identified in 5 patients: 1 patient had cytomegalovirus hepatitis and the remaining 4 cases were defined, by exclusion, as non-A, non-B hepatitis (with prevalence and incidence rates of 80% and 6.25% respectively). We found no statistically significant differences between the numbers of transfused blood product units in patients who developed non-A, non-B hepatitis as compared to those who did not. Our analysis of the predictive effectiveness of alanine aminotransferase and anti-HBc antibodies screening in blood donors to prevent non-A, non-B post-transfusion hepatitis led to the following conclusions: we failed to confirm the association between anti-HBc in blood donors and enhanced risk of non-A, non-B hepatitis in recipients since no case developed among patients receiving blood products from anti-HBc positive donors. So, 20 donors (4.5%) would have been discarded without any reduction of the incidence of non-A, non-B hepatitis. we could not confirm nor exclude the possibility that screening donor blood for elevated alanine aminotransferase levels would have reduced the number of non-A, non-B hepatitis in recipients.

Adult↗