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F Stratton

Publications and source records attributed to F Stratton.

48 records · Page 3Linked to original sources
PubMed1968

Value of gel filtration on Sephadex G-200 in the analysis of blood group antibodies.

Analysis of blood group antibodies on Sephadex G-200 columns is described. The position of blood group antibodies when whole serum containing them is subjected to gel filtration is studied and compared with the position of the gamma-globulins and of standard blood group antibodies. The technique on its own cannot be used to identify the immunoglobulin nature of the antibody but is very useful when employed in conjunction with the antiglobulin test using specific antisera and with other methods. The technique is rapid, simple, and reliable.

ABO Blood-Group System↗
PubMedDate not supplied

Test cells for use with anti-IgG sub-typing antisera in the antiglobulin test.

The preparation of test cells coated with specific IgG of known subclass is described. Such cells are required in the standardization of IgG subtyping reagents. At present, these test cells usually are prepared by coating cells with IgG myeloma paraproteins. However, these paraproteins may not be generally available and an alternative method is presented using more readily obtainable materials. Quantification of cell-bound IgG showed that the subclass did not affect the sensitivity of the antiglobulin test when using broad-spectrum anti-IgG and that the test cells produced had an optimal IgG coating. Reactions with subclass-specific antisera were however, considerably weaker that those obtained with broad-spectrum anti-IgG. A modified spin-layering technique for use with subclass-specific antisera is described.

ABO Blood-Group System↗