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F Staib

Publications and source records attributed to F Staib.

At least 73 records · Page 4Linked to original sources
PubMed1982

Bird manure filtrate agar for the formation of the perfect state of Cryptococcus neoformans, Filobasidiella neoformans. A comparative study of the agars prepared from pigeon and canary manure.

An account is given to two pairs of C. neoformans strains on two different bird manure filtrate agars, i.e. pigeon manure filtrate and canary manure filtrate agar. Basidiospore formation was observed in both pairs after 3 d incubation at 26 degrees C on both media. 0.1% biphenyl in pigeon manure filtrate agar showed an inhibitory activity on the formation of the perfect state of the C. neoformans pairs tested. Concentrated pigeon manure filtrate allowed growth of the imperfect, however not of the perfect state of C. neoformans. On canary manure filtrate agar, a brown color effect (BCE) of the colonies of the C. neoformans strains was observed in addition to their formation of the perfect state. Except for a known self-fertile strain no self-fertility could be seen in 40 C. neoformans strains on pigeon manure filtrate agar. Attention is drawn to the risk of laboratory infection when handling the perfect state of C. neoformans.

Agar↗
PubMed1982

[Mycoses caused by fungal spores in indoor air].

Airborne fungal spores may cause mycoses and allergies. From the epidemiological angle, various modes of exposure are possible, viz. 1. Professional (Work with material contaminated with fungi); 2. Accidental (Growth or presence of fungi owing to shortcomings of construction); and 3. Discrete, constant (Household-associated activities such as keeping of potted plants and pets). To enable an evaluation of indoor air quality in respect of its content of fungal spores, a reliable mycological diagnosis is required.

Air Microbiology↗
PubMed1981

[Chronic pulmonary cryptococcosis. A case contribution for diagnosis of cryptococcosis in man from a pathologic-anatomic viewpoint].

Routine use of differential nutrients for isolating cryptococcus neoformans (Staib-Agar) during post-mortem examination revealed previously unsuspected chronic pulmonary cryptococcosis in a 64-year-old woman. Cause of recurrent pleural effusions over three years were probably sub-pleural cryptococcomas. In on-target and specific search for the cryptococcosis pathogen, the lung deserves special attention as a probable gateway.

Autopsy↗
PubMed1981

Effect of uremia on lymphocyte transformation and chemiluminescence by spleen cells of normal and Cryptococcus neoformans-infected mice.

The effect of uremia on immune incompetence was studied. BALB/c mice were infected with a minimally virulent strain of Cryptococcus neoformans 6 weeks before immune assay. Uremia was induced by intramuscular injection of 0.15 ml of glycerol. Pooled spleen cells from four experimental groups (normal, uremic, infected, and infected and uremic) were assayed by lymphocyte transformation (LT) and luminol-dependent chemiluminescence (CL) 24 h after induction of uremia. A greater response to phytohemagglutinin and concanavalin A stimulation in tests of LT and CL was exhibited by uremic cells than by nonuremic cells; however, the presence of BALB/c uremic serum resulted in lower responses by both LT and CL. Infected mice showed a greater response to mitogens than did noninfected mice, but no significant stimulation in response to heat-killed whole cells of C. neoformans. Spleen cell populations of uremic mice had a lower viability and a different composition of spleen cell subpopulations than did cell preparations from nonuremic mice.

Animals↗
PubMed1981

Serotypes of Cryptococcus neoformans strains isolated in Germany.

An examination of 21 strains of Cryptococcus neoformans isolated from environmental and clinical sources in Germany revealed only serotypes A, D, and AD. Of these, 13 isolates were serotype A, 5 isolates were serotype D, and 3 isolates were identified as serotype AD. The absence of serotypes B or C confirms earlier reports from other European countries.

Animals↗
PubMed1981

The perfect state of Cryptococcus neoformans, Filobasidiella neoformans, on pigeon manure filtrate agar.

To enable studies of the dependence of Cryptococcus neoformans and its perfect and imperfect states upon bird manure as a habitat of this pathogen, a nutrient medium closely resembling natural conditions was prepared. As sole nutrient, the water soluble ingredients of manure from pigeons (Columbia livia) were used. There was no heat sterilization of the manure filtrate. Using a standard pair of C. neoformans strains for mating, it could be demonstrated that the perfect state of the fungus developed on this so called pigeon manure filtrate agar within 48 h at 26 degrees C. This medium is supposed to help in the elucidation of the epidemiological significance of the perfect and imperfect states of this pathogen.

Agar↗
PubMed1980

[Aspergillus fumigatus infection of the lung in cystic fibrosis (author's transl)].

In an 8-year-old girl with cystic fibrosis Aspergillus fumigatus infection of the lung was diagnosed. Pot-plant soil as reservior of Aspergillus fumigatus was shown to be the source of inhalable Aspergillus fumigatus units within the clinic. Cystic fibrosis mucus of the patient acted as nutritive substrate for Aspergillus fumigatus. Consequent exclusion of pot plants in housing and hospital rooms is considered necessary for prevention of Aspergillus fumigatus infection in patients with cystic fibrosis.

Aspergillosis, Allergic Bronchopulmonary↗
PubMed1980

Interaction between aspergilli and streptomycetes in the soil of potted indoor plants: a preliminary report (contribution to the epidemiology of human aspergillosis).

The soil of potted ornamental plants as a reservoir for Aspergillus species pathogenic for man is of epidemiological and ecological interest. Isolation of A. niger as the sole hyphomycete from the soil of potted African violets (Saintpaulia ionantha, Gesneriaceae), prompted us to look for A. inger on the surface of the roots of this plant. Small pieces of the roots were inoculated in the nutrient-free agar-gel with and without antibiotics. On the antibiotic-free gel, a dense growth of Streptomyces griseus was consistently observed around the root pieces. But the gel fortified with antibiotics showed pure growth of only A. niger. One of the two strains of S. ariseus isolated from the African violet, showed inhibitory effect against a number of fungi including A. niger but the other strain had no effect on this fungus. The possible influence of streptomycetes on the occurrence of aspergilli in the soil of potted ornamental plants is discussed.

Aspergillosis↗
PubMed1980

A notable Aspergillus from a mortal aspergilloma of the lung. New aspects of the epidemiology, serodiagnosis and taxonomy of Aspergillus fumigatus.

Different basic mycological observations were made in relation to a fatal case of aspergilloma of the lung in a 45-year-old male. Cultural examination of the sputum and autopsy material from the lung revealed the predominant presence of a white fungus along with a scarce growth of the typical Aspergillus fumigatus. The white isolate produced conidial heads almost similar to that of A. fumigatus but with apparent septation in the phialides. With the help of the immunodiffusion test and use of the antigens from the typical A. fumigatus strain and from the white isolate, antibodies could be detected in the serum of this patient and an antigenetic relationship between these fungi could be observed. It is assumed that this white isolate is a variant of A. fumigatus and its presence in the lung is an indication of chronic or progressed aspergillosis caused by A. fumigatus. Cultural characteristics and developmental morphology of this fungus and the serodiagnostic value of the antigen derived from it will be described in detail in separate communications.

Antibodies, Fungal↗
PubMed1980

Auxanographic detection of experimental murine uremia with Cryptococcus neoformans.

A modified blood residual nitrogen plate auxanographic method was applied to the detection of experimental uremia in a murine model. The yeast-like fungus, Cryptococcus neoformans, was used as the indicator. Transient uremia was induced by injection of 0.2 ml glycerol intramuscularly. The low molecular weight nitrogen levels were estimated by measuring the diameter of the auxanogram at intervals of 2 hr for 24 hr and at 32 hr after the glycerol injection. After 4 hr, elevated levels of low molecular weight nitrogen were found. Maximum levels occurred 20 hr post glycerol injection. This method requires only 5 microliter of whole blood per assay. The results can be read after an incubation time of 24 hr at 26 degree C. The stability of the prepared plates was determined to be at least 96 hr at 4 degree C. The ease of use, reliability and versatility of the modified auxanographic method are discussed.

Animals↗
PubMed1980

Pathogenic yeast-like fungi in meat products.

100 samples of sausage and ham (cut in fine slices) were examined to find out whether meat products can harbour yeast-like fungi pathogenic for man. Pieces of the test material were placed in a sterile Petri dish containing 1 ml distilled water and incubated at 26 degrees C for 2 days. The colonies grown on the material were identified by international standard methods, tested for extracellular proteolytic activity and serotypes. Experimentally infected meat products were studied culturally and histologically (PAS stain). 14 out of the 100 samples examined were found to contain Candida parapsilosis and 1 C. tropicalis. In vitro, extracellular proteolytic activity was found in 54.5% of the C. parapsilosis isolates. By serotyping, 2 of the isolates wree found to be related with serotype A and 8 with serotype B of C. albicans. The strains isolated from the blood of 27 patients, in part of them, identity of serotype and proteolysing activity was established. In the experimentally infected meat products, C. albicans showed a scarce growth preferably in the pseudomycelium form. C. tropicalis grew very well on the boiled sausage of salami type but mostly in the pseudomycelium form. C. parapsilosis on the other hand, showed a strong growth preferably as round blastospores on all 3 meat products and Cryptococcus neoformans a moderate growth as round blastospores in all the 3 meat products (Bologna type sausage, boiled sausage of salami type and smoke ham). The findings are discussed with a view of their implications for Medical Mycology, epidemiology and the practice of food control.

Blood↗
PubMed1979

[Localised aspergillosis and oxalosis of the lung caused by aspergillus niger: soil of ornamental plants as a reservoir of aspergilli (case report) (author's transl)].

Haemoptysis occurred in a 64-year-old man with a history of pulmonary tuberculosis, negative for M. tuberculosis. Precipitating antibodies to Aspergillus niger were found in serum and the fungus found in sputum. Autopsy revealed aspergilloma, colonization of the pulmonary cavity by A. niger and local oxalosis if the cavity wall. Ornamental plant soil containing A. niger conidia, as well as the air in the patient's living-room, was the probable source of infection.

Aspergillosis, Allergic Bronchopulmonary↗