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Biomedical subjects

F Stadil

Publications and source records attributed to F Stadil.

At least 55 records · Page 3Linked to original sources
PubMed1983

Circulating glucagon after total pancreatectomy in man.

In five totally pancreatectomized human subjects the secretion of gut-derived glucagons was stimulated by ingestion of a meal rich in fat and carbohydrates. Glucagon-like immunoreactivity in plasma, measured with an antiserum against the 6-15 sequence, increased fivefold in response to the meal. Glucagon like immunoreactivity measured with a antiserum against the C-terminal sequence was initially normal (12-13 pmol/l), increased slightly (to 20 pmol/l), and then decreased (to approximately 6 pmol/l). The chromatographic profile of glucagon-like immunoreactivity in plasma at maximum stimulation was studied after concentration by affinity chromatography. Both assay systems identified two peaks (at Kd-values of 0.30 and 0.60-0.65, and 0.30 and 0.70, respectively). The position at Kd 0.70 corresponds to that of glucagon 1-29. The same components may be identified in plasma from normal subjects. It is concluded that the human intestine is capable of generating all of the molecular forms of glucagon which normally are present in plasma.

Adult↗
PubMed1983

Sulfation of gastrin in Zollinger--Ellison sera: evidence for association between sulfation and proteolytic processing.

Sulfated gastrins were quantitated in sera from 15 patients with the Zollinger--Ellison syndrome (ZES) by specific radioimmunoassays. The total concentration of gastrin varied from 174 to 285 000 pmol/l. Sulfated gastrins constituted 44.8 +/- 5.5% (mean +/- S.E.M.) of the gastrins in ZES sera compared with 37.7 +/- 1.9% in sera from 100 control subjects (P greater than 0.1). There was no correlation between gastrin concentration and sulfation (r = 0.40). Gel and ion-exchange chromatography showed that up to 90% of the gastrins could be in the sulfated form. The highest degree of sulfation was found in sera where the small gastrin components dominated. Thus, the percentage of small gastrins (G-17 and G-14) correlated with the degree of sulfation (N = 15, r = 0.75, P less than 0.01). We suggest therefore that proteolytic processing of the gastrin precursor and sulfation of tyrosyl are associated.

Female↗
PubMed1983

Preparation of 125I-(Tyr 3)- and 125I-(Tyr 11)- neurotensin for radioimmunoassay.

The chloramine-T method for radioiodination of neurotensin for radioimmunoassay was studied. As conventional procedures produced heterogeneous preparations, labelling was performed with a low amount of chloramine-T (1.8 nmol) in the presence of excess of peptide (6 nmol). Purification and complete separation of labelled from unlabelled peptide was obtained by ion-exchange chromatography on SP Sephadex C-25. Four labelled components were identified by isoelectric focusing, enzymatic cleavage and studies of immunoreactivity. The two components representing monoiodinated preparations labelled at Tyr 3 or Tyr 11 could be isolated. Depending on the binding site of the particular antiserum the appropriate tracer could be selected for use in the radioimmunoassay. The specific radioactivities were high (2303 (2137-2407) microCi/nmol and 1927 (1608-2307) microCi/nmol (median and range] and the stability of the label and the reproducibility of the procedure was good.

Iodine Radioisotopes↗
PubMed1983

Gut and pancreatic hormones after jejunoileal bypass with 3:1 or 1:3 jejunoileal ratio.

The aim of the study was to elucidate the differential role of the jejunum and ileum in the regulation of secretion of the gut hormones, gastrin, gastric inhibitory polypeptide, and enteroglucagon, and the pancreatic hormones, insulin, glucagon, and pancreatic polypeptide, in man. We measured the plasma levels of the hormones (and glucose) during fasting and after a test meal in 34 obese patients, of whom 5 were waiting for bypass surgery and 29 had had a jejunoileal bypass with a 3:1 or 1:3 jejunoileal ratio between the functioning segments 3, 9, or 15 months earlier. The major findings were that surgery bypass (1) has no important influence on the levels of gastrin and pancreatic polypeptide, (2) reduces the level of gastric inhibitory polypeptide, insulin (and glucose), and enhances the pancreatic glucagon level, independently of the jejunoileal ratio, and (3) increases enteroglucagon secretion, most effectively so with a short jejunal and long ileal segment left in continuity. These findings suggest that the upper jejunum and terminal ileum has no important role in regulation of secretion of these hormones apart from that in secretion in enteroglucagon which is related to the length of functioning ileum.

Adult↗
PubMed1983

Neurotensin inhibits meal-stimulated gastric acid secretion in man.

The effect of physiological doses of exogenous neurotensin on meal-stimulated gastric acid secretion and serum gastrin concentration was investigated in six healthy subjects. Acid secretion was reduced by 32% during intravenous infusion of neurotensin, the plasma neurotensin concentration being within physiological range. Serum gastrin concentration was unchanged during infusion of neurotensin. The results strongly suggest that neurotensin participates in the regulation of gastric acid secretion and support the theory that neurotensin may play a role in the intestinal phase of gastric acid secretion in man.

Adult↗
PubMed1982

Prolonged secretory inhibition during cimetidine treatment in Zollinger-Ellison patients.

In 23 Zollinger-Ellison patients who had been treated continuously with cimetidine for more than 3 months repeated measurements of basal acid output (BAO) were carried out 10-12 h after the last drug dose, to evaluate the prolonged inhibition of gastric acid during continuous cimetidine treatment. Most patients had a markedly prolonged inhibition of BAO after a few weeks of treatment compared with the BAO before cimetidine treatment. The prolonged inhibition became significant after 6 to 12 months of treatment (p less than 0.05). In some patients the prolonged inhibition was transient. In seven patients cimetidine therapy was discontinued for 3 days. Measurement of BAO after 12, 36, and 60 h of withdrawal revealed a marked inhibition lasting more than 60 h in four patients. Determination of plasma cimetidine could not demonstrate any detectable amounts after 12 h of withdrawal. The prolonged inhibition of BAO observed during continuous cimetidine treatment is of significant importance for evaluating secretory data. The phenomenon is still unexplained.

Adult↗
PubMed1981

Ultrasonically guided percutaneous interstitial implantation of iodine 125 seeds in cancer therapy.

A new method for ultrasonically guided percutaneous interstitial implantation of 125I seeds in abdominal tumours is described. A linear array puncture transducer mounted on a special x-y-z co-ordinate frame is used in combination with a special insertion technique. Following in vitro experiments the technique was applied in two patients with pancreatic cancer. There were no immediate complications after the implantation of 36 and 37 125I seeds.

Aged↗
PubMed1980

Correlation between exocrine pancreatic secretion and serum concentration of human pancreatic polypeptide in chronic pancreatitis.

Concentrations of human pancreatic polypeptide (HPP) in serum were measured in the fasting state and after a meal in 19 control subjects and 24 patients with chronic pancreatitis (CP). The severity of CP was characterized on the basis of the duodenal lipase concentration after a test meal (Lundh test). Basal and postprandial HPP concentrations were significantly (p less than 0.01) decreased in severe and moderate chronic pancreatitis and in diabetes secondary to pancreatitis. There was only a weak correlation (r = 0.44; p less than 0.05) between exocrine secretion and delta HPP in CP. Fifty-eight percent of patients with CP had serum concentrations of HPP within the normal range, limiting the value of serum HPP measurement in the diagnosis of CP.

Adult↗
PubMed1980

Pancreatic ascites. Intraoperative localization of the pancreatic fistula.

Pancreatic ascites can be diagnosed by demonstration of high amylase concentration in the ascites and ERCP may serve to identify the causal internal fistula. Intraoperatively a precise localization of the ductal leakage is helpful. In two patients we demonstrated the fistulas by stimulation of the pancreatic secretion with secretin.

Adult↗
PubMed1980

Cancer. A prospective study of patients' opinion and reaction to information about cancer diagnosis.

When asked before admission to hospital 74% of 640 surgical patients suffering from various abdominal diseases stated that they wanted to be told if examinations revealed they were suffering from cancer. A prospective study of 58 patients confirmed this wish and no regrets were noted 3 days and 3 months later. The immediate reactions to information were moderate and almost all patients adjusted well to the situation after a short, varying, initial phase with anxiety. No severe, untoward reactions were seen. In contrast to the traditional attitude in surgical wards this study suggests that generally cancer patients should be given the diagnosis.

Adaptation, Psychological↗
PubMed1979

Small cell carcinoma of the lung: relation of calcitonin to bone marrow metastases, parathormone and gastrin.

The relations of calcitonin concentrations to the presence of bone marrow metastases and to the concentrations of calcium, parathormone and gastrin in serum were investigated in 74 untreated patients with small cell carcinoma of the lung. Calcitonin concentrations were enhanced in two thirds of the patients, while serum calcium concentrations were normal in all. In 19 of 57 patients parathormone concentrations were slightly above the normal range, but the concentrations of parathormone and calcitonin were not correlated. Bone marrow metastases had no influence on the concentration of serum calcitonin. Finally, a small inverse correlation between the concentrations of gastrin and calcitonin in serum was observed. The results resemble those of the calcitonin-producing medullary carcinoma of the thyroid, supporting the suggestion of an ectopic source of hypercalcitoninemia in small cell carcinoma of the lung.

Bone Neoplasms↗
PubMed1979

Plasma enteroglucagon after jejunoileal bypass with 3:1 or 1:3 jejunoileal ratio.

Enteroglucagon concentration in peripheral blood was determined before and after a test meal in 24 morbidly obese patients. Eighteen had jejunoileal bypass, 6 with a 3:1 and 12 with a 1:3 jejunoileal ratio of the functioning segment, and 6 were unoperated. All three groups exhibited an increment of enteroglucagon concentration after the meal. Both the fasting values and the postprandial integrated increments were higher in operated patients than in unoperated patients and higher after 1:3 bypass than after 3:1 bypass. The findings agree with the hypothesis that enteroglucagon secretion is stimulated by exposure of the lower bowel to upper-bowel content, and that the effect of enteroglucagon is, as seen after bypass operation, stimulation of growth and reduction of motility of the intestine.

Adult↗