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F Squartini

Publications and source records attributed to F Squartini.

83 records · Page 5Linked to original sources
PubMedDate not supplied

Characteristics of lymphomas in the BALB/cfRIII mouse strain.

The occurrence of 392 lymphomas in 1607 BALB/cfRIII mice from F0 to F45 is analyzed. Lymphoma incidence increased rapidly in the first 5 generations, reached a high plateau from F6 to F20 and then decreased slowly until it disappeared altogether. After F35, only sporadic cases of lymphoma have been observed. The morphologic characteristics of 350 of these lymphomas observed from F0 to F20 are described. Three main types of lymphomas have been recognized on the basis of gross morphology, histology and age: a) early, lymphocytic, with thymic involvement; b) early, lymphocytic, without thymic involvement; and c) late, histiocytic, without thymic involvement. The first 2 types are virus-induced and thymus-dependent, and the third type is both virus and thymus independent. Lymphomas without thymic involvement and histiocytic lymphomas increased with the generations. The source of causative virus, the interference with mammary tumors, and the possible cell types of origin of lymphomas are discussed.

Age Factors↗
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Quantitative comparison of milk-released C3H and RIII mammary tumor viruses in infected BALB/c hosts.

The C3H and RIII mammary tumor viruses (MTV) carried by BALB/cfC3H and BALB/cfRIII breeding females have been quantified and compared in milk samples, after partial purification with a sucrose density gradient. The samples were collected at identical times during the first 3 lactation periods from individual mice (6 per strain), standardized for age at delivery and size of litter. Milk samples from 6 MTV negative BALB/c controls have also been analyzed. Data for comparison are expressed in optical density units (ODU) and refer to the protein content of the whole milk using MTV-negative Balb/c milk as blank. The results have shown 1) an increase of MTV released through milk in each MTV-carrying female from the first (average ODU, 0.542) to the second (1,351) and third (2.105) lactation, 2) individual variations, and 3) a significant difference in release between C3H and RIII MTV, the latter being more than double (average ODU, 1.801) in respect to the former (0.847). The apparent discrepancy between these results and the bioactivity in BALB/c mice of C3H and RIII MTV, significantly lower for the latter, is discussed.

Animals↗
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Value of ER-D5 immunocytochemical determination in routine tissue sections of human breast cancer.

ER-D5 is a recently identified protein related to estrogen receptors (ER). Generally ER measurement requires fresh frozen tissue and for ER-D5 assay ethanol (E) fixation of the specimen is recommended. We evaluated the possibility of immunocytochemical detection of ER-D5 in routine formalin-fixed (F) sections in 51 breast cancers comparing the results with those obtained in the same specimens using E as fixative. The results of ER-D5 assay were expressed by the staining index (SI) taking values greater than or equal to 5 as positive. In all tumors ER was also assayed by a biochemical method (DCCA). The sensitivity of ER-D5 detection in F was only 33.3%, while the specificity was 94.4%. A lower cut-off value of SI for F sections (greater than or equal to 2) increased the sensitivity to 66.6%, leaving the specificity unchanged. A strong correlation was found between the SI of ER-D5 in E and F (p less than 0.001). The SI of ER-D5 in F sections was also well correlated with ER concentrations (p less than 0.001). These results suggest that immunocytochemical determination of ER-D5 in routine sections may be useful in retrospective studies of hormone dependence in breast cancer.

Adult↗
PubMedDate not supplied

Estrogen, progesterone receptors and proliferating activity evaluated by immunocytochemistry in breast cancer.

The correlation of the most important prognostic indicators was evaluated in 75 breast cancer cases. Estrogen-progesterone receptors and proliferating activity were analyzed by immunocytochemical methods (ER-ICA, PR-ICA, Ki-67). Both steroid receptors were inversely correlated with the proliferating activity (ER-ICA vs Ki-67, p less than 0.003; PR-ICA vs. Ki-67, p less than 0.0001). No correlation was found between steroid receptors or cell kinetics and tumor size or lymph node status. These findings confirm the relevance of biochemical and kinetic parameters as independent markers in breast cancer and suggest a routine use of the simple immunocytochemical methods in assessing the biological behavior of tumors.

Adult↗
PubMedDate not supplied

Correlations between histologic type of clinical breast cancer and physiopathologic profile of the mammary glandular tree.

The relationships between cancer histotype and characters of the mammary glandular tree have been studied in 100 human breasts removed for clinical cancer by radical mastectomy. The group of invasive ductal carcinomas, including 78 cases, was subclassified in not otherwise specified (53 cases), with intraductal (19 cases), and with tubular component (6 cases). The other cancer histotypes were invasive lobular carcinoma (9 cases), medullary carcinoma (7 cases), mucinous carcinoma (4 cases), and papillary carcinoma (2 cases). The pattern of the mammary glandular tree was atrophic (67 cases) or adenosic, i.e., rich in mammary lobules (33 cases), but mammary lobules (normal, paranormal, atypical, or persistent in otherwise atrophic breasts) were present in 83% of the cases. A significant prevalence of not otherwise specified cancer (X2 = 10.20; p less than 0.01) was found in atrophic breasts (64%) and, even more, in breasts without lobules (76%). By contrast, intraductal component, tubular component, invasive lobular, and medullary cancers were, with two single exceptions, only in breasts with lobules, and this concentration was also significant (X2 = 7.26; p less than 0.01). It is concluded that breast cancer histotype is influenced by, or related to, the surrounding microenvironment, i.e., the pattern of the mammary glandular tree.

Adult↗
PubMedDate not supplied

The microenvironment of human breast with clinical cancer.

The characteristics of the mammary glandular tree have been studied in 100 human breasts removed for clinical cancer by radical mastectomy. The glandular tree was observed in whole thin (2 mm) stained sections under a stereoscopic microscope with removal of any suspicious or interesting change for histology. The results allow to categorize as follows the microenvironments in which mammary neoplasia may clinically appear. In respect to the main characteristics of the collateral glandular tree, clinical breast cancer may occur in: (a) atrophic breasts without lobules or lesions in 17% of cases; (b) atrophic breasts with persistent lobules and minor pathologic changes such as cysts, apocrine metaplasia, sclerosing adenosis, fibroadenoma and ductal hyperplasia in 19% of cases; (c) atrophic breasts with proliferative lobular changes (atypical lobules), duct papillomas, in situ carcinomas and/or microscopic independent foci of infiltrating carcinoma in 31% of cases; (d) adenosic breasts, which are breasts rich in mammary lobules, with the minor pathologic changes specified under (b) in 14% of cases; (e) adenosic breasts with the severe proliferative changes specified under (c) in 17% of cases, and (f) adenosic breasts with normal lobules and without lesions in 2% of cases. Therefore, 83% of the cancerous breasts have normal or pathologic lobules and 81% have lesions of various degrees of severity in the glandular tree.

Adult↗
PubMedDate not supplied

p53 expression in non small cell lung cancer: clinical and biological correlations.

p53 is a tumor suppressor gene, located in the short arm of chromosome 17, which encodes for a nuclear protein involved in the control of cellular growth. Mutations in p53 gene are the most common genetic alterations in a several human cancers, including Non Small Cell Lung Cancer (NSCLC). However, up to now, the role of p53 in the tumour's behaviour and its progression has not been completely clear. We performed immunohistochemical staining for mutated p53 using two monoclonal antibodies, PAb1801 and PAb240, in fresh tumour specimens from 103 consecutive patients who underwent surgery for resectable NSCLC. PAb1801 detects both the normal and mutant form of p53, while PAb240 is specific only for the mutant form and recognizes a denaturation-resistant epitope located between aminoacids 156-335. Both antibodies showed a mainly nuclear staining in neoplastic cells but not in surrounding uninvolved lung tissues. 68 out of 100 (68%) and 37 out of 103 (35.9%) of the cases were positive with PAb1801 and with PAb240, respectively. Tumours from patients with hilar-mediastinal lymph node involvement showed a higher p53 expression, detected by PAb1801, than those without nodal metastases (p = 0.04). Moreover, tumours expressing more than 60% of positive cells with both antibodies showed a significant increase of nodal involvement (p = 0.1; p = 0.03). Furthermore, p53 expression was significantly related to post-surgical stage (p Tumor Stage) (p = 0.04). In addition, we did not find any correlation between p53 expression and proliferating activity evaluated by PCNA, Ki-67 and DNA flow cytometric cell cycle. In conclusion, the evaluation of p53 oncogene expression may identify individuals whose resectable NSCLCs have a more aggressive tumour behaviour.

Antigens, Neoplasm↗