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Biomedical subjects

F Sperling

Publications and source records attributed to F Sperling.

11 recordsLinked to original sources

River basin management, described for the example of the Emschergenossenschaft.

Considerable problems concerning drainage capacity made it necessary, at the beginning of the 20th Century, to make a joint public-private effort, because the existence of the Ruhr Area as an industrial location was endangered. By Prussian law a co-operative was established for wastewater and drainage-management of the entire Emscher river system. In the past the Emscher Association and the later founded Lippe Association have proved to be the best instrument to cope with the wastewater problems of a densely-populated industrial area. In recent time, the Emscher area is undergoing fundamental structural changes. To increase its attractiveness increased efforts are needed for a modernisation of the wastewater transport and treatment system. To deal with the various and complex tasks to reshape the Emscher system, the joint association again provides a highly efficient and competent instrument.

Conservation of Natural Resources↗

Anti-inflammatory effects of systemic anti-tumour necrosis factor alpha treatment in human/murine SCID arthritis.

OBJECTIVES: To evaluate in vivo the contribution of tumour necrosis factor alpha (TNFalpha) to the chimeric transfer model of human rheumatoid arthritis synovial membrane into SCID mice (hu/mu SCID arthritis), systemic anti-TNFalpha treatment was performed and the clinical, serological, and histopathological effects of this treatment assessed. METHODS: Animals were treated with the rat-antimouse TNFalpha monoclonal antibody V1q, starting on day 1 after hu/mu engraftment, twice weekly for 12 weeks. Joint swelling, serum concentrations of human and murine interleukin 6 (IL6), and serum amyloid P (SAP) were measured. Histopathological and immunohistochemical analyses of the joints were also performed at the end of treatment. RESULTS: Neutralisation of murine TNFalpha induced the following effects: (a) reduction of extent and duration of the acute arthritis phase, with significant reduction of joint swelling at two weeks; (b) decrease of murine SAP concentrations after the first antibody administration; and (c) increase of murine IL6 in the serum. At the end of treatment, there was a significant reduction of the inflammatory infiltration in the engrafted joints. Because of the mild degree of joint erosion, no treatment effects could be demonstrated on the destructive process. CONCLUSION: In the lymphocyte independent hu/mu SCID arthritis, anti-TNFalpha treatment reduces local and systemic signs of inflammation.

Acute Disease↗

Developmental toxic effect after subcutaneous injections of methadone in Charles River CD-1 mice.

Four groups of Charles River CD-1 pregnant mouse dams were dosed subcutaneously daily with 5, 10, 20, and 28 mg/kg of methadone during day 6 to 15 of gestation. Prenatal and postnatal studies were conducted in the offspring and mouse dams. Caesarean sections were performed on some of the females from each group on day 19 of gestation and fetuses evaluated by routine teratologic methods. All females remaining were allowed to deliver, and several postnatal signs were recorded from the pups during the lactation period. Maternal mean body weight gains and food consumption were reduced. No apparent alteration in the food efficiency index was detected, thus maternal malnutrition may not have been a factor in producing adverse effects on offspring. The incidence of external and visceral malformations was neither dose-related nor significantly different from those of the controls. However, administration of methadone decreased ossification of the digits, sternum, and skull of the offspring. Adverse effects of methadone on reproduction were apparent at doses of 20 mg/kg/day and above, and characterized by an increase in resorptions and a decrease in the number of live fetuses per litter. Methadone was found not to be teratogenic to the CD-1 mice. However, adverse effects on intrauterine growth, fetal ossification, neonatal growth, development, and survival rate were seen among weaned pups.

Animals↗

Pregnancy-associated plasma proteins in pregnant and pseudopregnant rats.

As in the human, the rat pregnancy-associated plasma proteins consisted of four distinct entities as revealed by immunological methods. All showed gradual increases during gestation and rapid disappearance postpartum. They could be physicochemically, although not immunologically, tentatively related to the human pregnancy proteins. None of them was detectable in the serum of rats at various stages of pseudopregnancy, nor in nonpregnant rats.

Animals↗

Biological parameters and the acute LD50 test.

The acute LD50 is an inadequate index of toxicity because the catastrophe of death obscures the mechanisms and sites of action. Thus, nonlethal toxicity is not evaluated, and the LD50 is not extrapolated to the possible effects of low level exposures for long periods of time. Collaborative studies should be undertaken which would develop methods to reasonably permit such evaluation and extrapolation.

Biological Assay↗