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Biomedical subjects

F Saji

Publications and source records attributed to F Saji.

At least 163 records · Page 9Linked to original sources
PubMed1984

Immunosuppressive factors in ascites fluids from ovarian cancer patients.

The nonspecific immunosuppressive effect of ascites fluids from ovarian cancer patients was examined and compared with that of noncancerous abdominal effusion and sera from ovarian cancer patients. The malignant ascites fluids produced a noncytotoxic, dose-dependent suppression of DNA synthesis of phytohemagglutinin-stimulated human peripheral blood lymphocytes. The suppression was higher than that observed in sera from cancer patients. No suppressive effect was seen in control abdominal effusion. The factors responsible for inhibition of in vitro lymphocyte function were partially purified from ascites fluid by lentil lectin affinity chromatography and gel filtration. Major active factors had a high molecular weight (440-1500 kilodaltons), an affinity to lentil lectin, and were stable against heat and acid treatment.

Ascitic Fluid↗
PubMed1982

A human thy-1-like antigen expressed on cultured leukemic T-cells.

A human cell membrane antigen that is highly T-cell specific among a number of leukocyte cell lines was isolated from cells of a human T-cell leukemia cell line, SKW-3. In addition to the high specificity to T-cell-type cell lines, the isolated antigen showed the following characteristics: (1) It is an acidic glycoprotein of approximately 30 000 daltons that has a charge heterogeneity and probably a disulfide bond(s); (2) Its antigenicity is stable when treated with heat, acid, and various protein denaturants; (3) It is widely distributed in lymphoid and nonlymphoid tissues but is most predominant in brain. These features are similar, if not identical, to those reported for the Thy-1 antigen of mouse or rat and have raised the possibility that this cell membrane antigen may correspond to human "lymphocyte" Thy-1 antigen, the counterpart of human "brain" Thy-1 antigen.

Antigens, Neoplasm↗
PubMed1980

[Argyrophil cell of the endometrial adenocarcinoma -light and electron microscopic observations (author's transl)].

Fifty one endometrial adenocarcinomas were used in this study. For light microscopic observations paraffin section from 43 cases were processed with H and E, PAS, and Grimelius argyophil reaction. Besides, 21 of these endometrial adenocarcinomas (25.6%) showed positive Grimelius' argyrophil reaction. With regard to the degree of histological differentiation, these 11 cases were classified as G1 or G2 and all G3 cases (2 cases) revealed negative for Grimelius argyophil reaction. Under electron microscope, in 3 cases out of 21, many small granules reminiscent of neurosecretory type granules mostly round in shape with high electron dense core so often surrounded by a limiting membrane were detected in some of the endometrial carcinoma cells having argyophil granules under light microscope. Since the distribution of these granules was assumed to be random however, the clusters of granules so often located in the groundplasm of subapical portion and subnuclear area near the epithelial basement membrane of the glandular cells. In high magnification, they consisted of an aggregation of fine granular substances with high electron density. Based on the morphological characteristics we made a comparative study between argyophil cell carcinoma of the endometrium and apudomas of the uterine cervix.

Adenocarcinoma↗
PubMed1980

Sensitized T-lymphocytes against paternal histocompatibility antigens cause intrauterine fetal death and growth retardation.

The influence on pregnancy of sensitized T lymphocytes against male histocompatibility antigens was investigated using inbred strain mice. Number of abortion, litter size, fetal weight and placental weight were studied on 18th day of pregnancy in the mice which were administered intravenously with T lymphocyte enriched fraction of sensitized spleen cells against allogeneic male histocompatibility antigens. The incidence of abortion in the experimental mice was higher than that in control mice. Mean fetal weight in the experimental mice was less than that in the control mice although mean placental weight in the experimental mice was unaffected. Those results show that immunologic interruption of pregnancy due to histoincompatibility between mother and fetus can occur and that the immunologic interruption of pregnancy is caused by sensitized T lymphocytes against paternal inherited histocompatibility antigens. Possible process of the immunologic interruption of pregnancy is discussed.

Animals↗
PubMed1980

[Vaginal smears of malignant ovarian tumors: comparison with cervical canal, aspirate uterine corpus, ascites, imprint smears and cytomorphology of histological specimens (author's transl)].

Vaginal smears of 21 patients with malignant ovarian tumors were examined. In 5 cases malignant cells were present: 1 mucinous cystadenocarcinoma, 2 poorly differentiated adenocarcinomas, 1 clear cell carcinoma, and 1 adenoid squamous cell carcinoma arising in a dermoid cyst. Cytomorphology of tumor cells in tissue specimens and imprint, ascites, aspirate uterine corpus, cervical canal and vaginal smears were examined. The ovarian malignant cells of the vaginal smears were grouped, round or oval with eccentric round or oval nuclei having a finely granular chromatin pattern regularly distributed, and with vesicular or vacuolated cytoplasm. Tumor diathesis appeared in 1 of 5 cases. Two post-menopausal patients showed an increase of superficial cells in the maturation index. In the comparison of malignant cells taken from different locations, vaginal tumor cells were similar to those in ascites. Since ascites smears were reported to be useful for the diagnosis of some kinds of ovarian tumors, it was expected that vaginal smears might also have this potentiality.

Adult↗
PubMedDate not supplied

Transvaginal ultrasonographic cervical assessment for the prediction of preterm delivery.

To determine the usefulness of transvaginal ultrasonographic cervical assessment for the prediction of preterm delivery in an apparently normal population, 729 pregnant women (between 15 and 34 weeks' gestation) were randomly enrolled in the study in ten tertiary perinatal centers in Japan. Cervical parameters, including cervical length, internal os dilatation, and funneling depth, were measured by transvaginal ultrasound. The predictive values of these measurements for preterm delivery were investigated in a prospective fashion. Among various cervical parameters, cervical length showed the best correlation with pregnancy outcome. Cervical length (mm) was gradually decreased as the gestational age progressed, the regression line being y = 41.21-0.22x. When the mean cervical length minus 1 standard deviation at each gestational age was chosen as a cut-off value, the group with a shortened cervix showed a significantly high preterm delivery rate exclusively in the primigravidae (odds ratio: 4.86, 95% CI: 1.85-12.72). Internal os dilatation, in contrast, was a useful predictor in multiparous women (odds ratio: 6.00, 95% CI: 1.65-21.71). It was concluded that tranvaginal ultrasonographic cervical assessment, especially the measurement of cervical length, was effective for the prediction of preterm delivery in the primigravidae.

Cervix Uteri↗
PubMedDate not supplied

A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiform mole.

A new method of DNA analysis has been used for the determination of androgenesis as a cause of complete hydatidiform mole. This method, using a minisatellite core probe, requires only a small amount of DNA and detects the restriction fragment length polymorphisms (RFLPs) due to allelic differences in the number of tandem repeats containing the core sequence. Southern blot hybridization showed an individual-specific DNA fingerprint, and each polymorphic band in molar tissues could be identified as being of paternal, but not maternal, origin. Some polymorphic bands of paternal DNA were not observed in molar tissues, indicating that endoreduplication of a normal haploid sperm or fertilization by dispermy to an anuclear oocyte with no effective genome could be the cause of complete hydatidiform mole. This method is sufficiently reliable and rapid that differential diagnosis could be made between complete hydatidiform mole, partial mole and hydropic change.

Adolescent↗
PubMedDate not supplied

Expression of HLA class I and beta 2-microglobulin on human choriocarcinoma cell lines: induction of HLA class I by interferon-gamma.

The trophoblast cells which contact with maternal tissue express HLA class I either weakly or not at all. This phenomenon is beneficial for the protection of the fetus from immunological rejection by the mother. However, the mechanisms of reduced expression of HLA class I are unexplained. Therefore, we utilized choriocarcinoma cells to analyse the suppressive mechanisms to HLA class I expression in trophoblasts. In this paper we reported the heterogeneity of HLA class I expression in five choriocarcinoma cell lines. The levels of surface HLA class I molecules correlate approximately with mRNA of HLA class I heavy chain, and all choriocarcinoma cells produce moderate amounts of surface and secreted beta 2 microglobulin. It was also found that interferon gamma upregulates HLA class I molecules and mRNA in two choriocarcinoma cell lines, NUC1 and HCCM5.

Actins↗
PubMedDate not supplied

Localization of three subtypes of Fc gamma receptors in human placenta by immunohistochemical analysis.

We investigated the localization of the three subtypes of (Fc gamma R) in the normal human placenta using immunohistochemical and immunocytochemical techniques with specific monoclonal antibodies. The analysis revealed that Fc gamma RI was expressed on Hofbauer cells, that Fc gamma RII was expressed on Hofbauer cells and endothelial cells of fetal vessels, while Fc gamma RIII was expressed on trophoblasts, especially syncytiotrophoblasts. Moreover, we demonstrated that the expression of Fc gamma RI and Fc gamma RII on Hofbauer cells and endothelial cells of the fetal vessels in the 1st trimester placenta was different from that in the 3rd trimester placenta. These results, therefore, indicate that the three subtypes of Fc gamma R in the human placenta may contribute to maintenance of placental functions, because each Fc gamma R molecule displays unique biological functions similar to those on leukocytes.

Animals↗
PubMedDate not supplied

Production of interleukin-6 by normal human trophoblast.

The placenta plays a number of important roles during pregnancy, some of which might be mediated by cytokines with multiple activities such as IL-6. Using an IL-6-dependent cell line, MH6o.BSF2, we showed that the placenta released IL-6 into the culture supernatant. Analysis of single-cell suspensions of placental cells determined the major source of IL-6 to be trophoblast. Using a mouse monoclonal antibody specific for IL-6 (alpha BSF2-I66), immuno-histochemical analysis of placental specimens demonstrated the localization of IL-6 only in the trophoblast layer. Additional immunocytochemical studies with single-cell suspensions of trophoblasts demonstrated the preferential presence of IL-6 molecules in syncytiotrophoblasts rather than cytotrophoblasts. The evidence that a high titer of IL-6 is produced spontaneously by syncytiotrophoblasts indicates that IL-6 may play immunological roles in fetomaternal interactions by means of IL-6-driven multiple immunoregulatory activities.

Cells, Cultured↗
PubMedDate not supplied

Selection of acrosome-reacted sperm with MH61-immunobeads.

Separation of acrosome-reacted viable sperm is an important problem in the analysis of sperm function and the micromanipulation of gametes such as the microinjection of sperm into ooplasm and subzonal sperm insertion. In the present study, we collected acrosome-reacted sperm selectively using paramagnetic immunobeads, referred to as MH61-beads, which attach themselves to the head of the acrosome-reacted sperm and are recovered by a magnet. The maximum number of sperm was recovered by the MH61-beads when 3 x 10(5) sperm/100 microliters and 6 x 10(5) MH61-beads were coincubated for 1 hour. The recovery of sperm was significantly correlated with the percentage of the acrosome reaction in the capacitation medium. After incubation for 12 hours, 2.4-9.0 x 10(4) sperm could be collected, and the percentage of viable and acrosome-reacted sperm was 28-60%. To improve the recovery of the acrosome-reacted sperm, we utilized follicular fluid, which induces the acrosome reaction. Addition of follicular fluid to the capacitation medium at a final concentration of 20% induced the acrosome reaction and increased the recovery of acrosome-reacted sperm significantly. In conclusion, separation of acrosome-reacted sperm by the MH61-bead-binding method could become a useful technique in research of sperm function and gamete micromanipulation.

Acrosome↗