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Biomedical subjects

F S Fluer

Publications and source records attributed to F S Fluer.

At least 19 recordsLinked to original sources
PubMed2006

[Influence of the food products, enriched with pectin, on properties of potentially patogenic representatives of the microflora of the large intenstine].

The treatment method of disbios suffered by patients with syndrome of irritated intestines with constipations was under research. The method was based on introduction of sour-milk beverage with addition of beet pectin in a dietary intake. The comparison product was kefir. The following developments were detected against the positive dynamics of clinical symptoms: improvement of microbiocenosis structure, reduction of potentially pathogenic germs, inhibition of pathogenic properties of enterotoxigenic Staphylococcus aureus. Comparison group results showed lower clinical efficiency and poorer effect of dietary treatment on large gut microorganisms, contents of enterotoxigenic S. aureus remained unchanged. Introduction of sour-milk beverage with addition of alimentary fibre in a dietary intake of patients suffering the syndrome of irritated intestines with constipations was found expedient.

Adult↗
PubMed2002

[Enterotoxin production as a diagnostic test in intestinal dysbacterioses ].

The new approach to advancing diagnostic of dysbacterioses grounded on determination of the pathogenic factors of potential-pathogenic microflora is proposed. The method of reacting of an indirect hemagglutination with usage of erythrocital diagnosticums ostend availability of production of staphylococcal enterotoxines of types A and B in 40% of the strains of staphylococcuses, isolated at dysbacterioses. The intensity of production of staphylococcal enterotoxines for strains of microorganisms, isolated at dysbacterioses and carrying of S. aureus, and at food borne diseases, is various. The availability of clinical developments of an intestinal dysfunction in faeces of the patients contacts with persistence of enterotoxigenics S. aureus, and their elimination--with successful police of the disturbed microbiocenosis.

Bacterial Infections↗
PubMed2002

[Detection and serological screening of conditionally-pathogenic enterobacteria isolated from food products and the environment].

The spread of some coliform bacteria in foods, the faeces of children and adults and in human environment was studied, and the evaluation of their toxigenic potential and serological properties was given. More then 160 strains of enterobacteria including 70 strains of Escherichia spp were described. The frequency of detection enterotoxigenic E. coli in foods--18%--was approximately similar an amount of ETEC strains which was isolated in humans (22%). 3 strains of E. coli) 157 were found in foods and in clinical samples (1.9%). The observed results have confirmed the proposal about the increasing of ETEC E. coli food-borne transmitting and dissemination its in the environment.

Adult↗
PubMed1990

[An immunoenzyme test system for determining the staphylococcal exotoxin of toxic shock].

A highly sensitive and specific enzyme immunoassay system for the determination of staphylococcal toxic shock exotoxin (TSE), permitting the detection of TSE at a concentration of 5-10 ng/ml, has been developed. The possibility of using this assay system for the selection of TSE-producing strains has been shown. 84% of staphylococcal strains under study have been found to produce TSE.

Carrier State↗
PubMed1989

[A rapid method of detecting the staphylococcal exotoxin of toxic shock].

The authors suggest a simple and rapid technique, staphylococci coagglutination, for rapid detection of the toxic shock exotoxin (TSE)-producing S. aureus strains. The method is based on the sensitization of formalin-treated Cowan-1 S. aureus cells with immunoglobulins to TSE. The reagent thus obtained coagglutinates with the supernatant of TSE-producing S. aureus tested strains. This method is more sensitive than the routinely used gel immunoprecipitation. The technique for preparing the supernatant of S. aureus tested cultures is also described.

Exotoxins↗
PubMed1989

[The frequency of the production of toxic-shock exotoxin by Staphylococcus aureus strains isolated in the USSR].

515 S. aureus strains, isolated from carriers and patients with staphylococcal infection in different regions of the USSR, were studied. Of these, 52.2% were found capable of producing exotoxin of toxic shock (ETS). The occurrence of the capacity for ETS production was the same among the strains isolated from the upper respiratory ways of carriers and from the purulent inflammatory foci of patients and little varied in staphylococci isolated in different regions. The study revealed that in strains sensitive to the typing phages of the International Set the capacity for ETS production occurred considerably more frequently than in nontyped cultures. No essential differences with respect to this sign between strains belonging to different phage groups were established.

Adult↗
PubMed1989

[Production of toxic shock exotoxin by Staphylococcus aureus strains resistant and sensitive to antibiotics].

Three hundred and ninety two strains of S. aureus isolated from bacteria carriers and patients with staphylococcal infections in different regions of the Soviet Union were investigated. 55.9 per cent of the isolates were able to produce exotoxin of toxic shock. No regular relation between resistance to definite antibiotics (tetracycline, chloramphenicol, benzylpenicillin, streptomycin, lincomycin, erythromycin, oleandomycin, gentamicin and methicillin) and the polyresistance range on the one hand and the ability to produce toxic shock exotoxin on the other hand was revealed.

Anti-Bacterial Agents↗
PubMed1988

[Identification of Salmonella enterotoxin by coagglutination reaction].

It has been shown that the intensity of enterotoxin production by various strains of Salmonella is different (it ranges from + to +). Thus, in addition to adhesive properties and skin permeability factors, the ability of various Salmonella strains to produce enterotoxin is one of the pathogenic factors of these microorganisms. Further biological characteristics of the infective agents will promote the detection of epidemiologically significant types of microorganisms during outbreaks of toxicoinfections.

Agglutination Tests↗
PubMed1986

[Staphylococcal toxic shock exotoxin (its isolation and characteristics)].

The method for obtaining the preparation of toxic shock exotoxin (TSE) has been developed. This method comprises the following operations: the sorption of the toxin from the culture fluid on Amberlite CG-50, elution, dialysis, gel chromatography in a column with biogel P-2, isoelectric focusing, and gel chromatography in a column with Sephadex G-75. TSE is a relatively thermostable protein with a molecular weight of 24,000. Its isoelectric point is 7.2. Monospecific antiserum to TSE with precipitating antibody titer equal to 1:16, identical to the reference serum (M. S. Bergdoll), has been prepared. This antiserum has shown no cross reactions with the homogeneous preparations of staphylococcal enterotoxins.

Animals↗
PubMed1984

[Isolation of Salmonella typhimurium enterotoxin in partially purified form and study of its properties].

S. typhimurium enterotoxin, partially purified in accordance with our scheme (salting out with 75% ammonium sulfate, dialysis and gel filtration in a column with Sephadex G-150, followed by electrofocusing), showed enterotoxic activity in the intestinal loop of a rabbit and yielded the positive result in the cutaneous test. S. typhimurium enterotoxin proved to be protein with a molecular weight of 140000 daltons and the isoelectric point equal to 4.4. The biological activity of S. typhimurium enterotoxin was neutralized with homologous antiserum and with antiserum to cholera enterotoxin. Heating the preparation at 75 degrees C for 30 minutes led to a considerable decrease in its enterotoxic activity.

Animals↗
PubMed1983

[Isolation of staphylococcal enterotoxin type B and a study of its physicochemical, biological and immunological properties].

The homogeneous preparation of type B staphylococcal enterotoxin (SEB) has been obtained by its adsorption from the culture fluid on amberlite, elution, isoelectric focusing in the ampholine system and gel filtration in a column packed with Sephadex G-25. The isolated SEB component is a thermostable protein, homogeneous in its antigenic composition and according to the results of disc electrophoresis in acrylamide gel. Its molecular weight is 28,000 daltons with pI equal to 8.6 (the main component), 8.0 and 8.25 (the minor components). All the forms are immunochemically identical. The minimum enterotoxic dose of SEB for cats is 3-5 micrograms per kg of body weight. The biological activity of the enterotoxin disappears under the action of 6 M urine. Monospecific antiserum to SEB with a precipitating antibody titer of 1:64 has been obtained.

Animals↗
PubMed1983

[Toxigenicity of microorganisms isolated from dry products for infant and dietetic nutrition].

The authors describe the results of studying the dissemination with Bac. cereus cells and staphylococci of raw material, components and ready-made products for infants' and dietetic nutrition after varying times of storage under the conditions of uncontrolled humidity and temperature. It has been demonstrated that Bac. cereus and staphylococcal strains isolated are potentially enterotoxigenic. Prevention of food poisoning with the microorganisms in question should be aimed at the improvement of industrial sanitary and hygienic conditions.

Bacillus cereus↗
PubMed1982

[Production of a monovalent staphylococcal enterotoxic antiserum type A and its use for typing staphylococcal enterotoxins].

A monovalent specific staphylococcal antiserum, type A, was obtained by means of the isolated and purified preparation of type A staphylococcal enterotoxin. This antiserum was proved to be identical to antiserum of the same type, manufactured by Serva Feinbiochemica GmbH & Co. (West Germany). The titer of the newly obtained antiserum in the precipitation test was 1 : 16, and its use allowed one to detect enterotoxin of the above-mentioned type at a concentration of 0.004 mg/ml. The study of 320 staphylococcal strains with the use of this antiserum revealed that 25 strains (7.8%) produced type A enterotoxin.

Enterotoxins↗