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Biomedical subjects

F Roussel

Publications and source records attributed to F Roussel.

At least 37 records · Page 2Linked to original sources
PubMed1992

Euonymus europaeus lectin as an endothelial and epithelial marker in canine tissues.

The Euonymus europaeus agglutinin (EEA) is an endothelial marker in mammalia. In canine tissues, 4 types of endothelial cells (general, nervous, arterial, hepatic) were identified by the presence of the EEA receptor and by its sensitivity to neuraminidase enhancement. In adult dogs, EEA binding saccharides had endothelial or epithelial distributions and reactivities similar to those described for human tissues. Different EEA reactivities were observed between fetal, neonatal and adult canine tissues mainly at the arterial level. These findings suggest that the development of the binding sites is not identical in dog and man. Related lectins and monoclonal antibodies were used to characterize the EEA binding site, and the probable structure of the EEA binding saccharide in endothelial cells appeared to be alpha Gal (1,3) beta Gal (1,4) GlcNAc.

Animals↗
PubMed1991

A cytopathic effect of Trichomonas vaginalis probably mediated by a mannose/N-acetyl-glucosamine binding lectin.

The pathogenic effect of a highly pathogenic strain of Trichomonas vaginalis on McCoy cell monolayers was investigated. Specific inhibition of the cytopathic effect by monosaccharides, such as N-acetyl-glucosamine (GlcNAc) and mannose (Man), was observed. Our preliminary results suggest that the pathogenicity of T. vaginalis depends on a lectin specifically sensitive to GlcNAc and to a lesser extent to Man. Although N-acetyl-mannosamine was found to be the most efficient inhibitor, this effect seems to be unrelated to the natural biological behaviour of the infested host.

Acetylglucosamine↗
PubMed1991

Granulomatous renal mass during endovesical BCG therapy for bladder carcinoma. Diagnosis by fine-needle aspiration.

One case of pseudotumoral granulomatous renal mass during endovesical bacillus Calmette-Guérin (BCG) therapy for superficial bladder neoplasm is reported. Such an adverse effect is exceptional and is clearly related to a vesicorenal reflux in our patient. In this case ultrasound-guided fine-needle aspiration was able to settle the diagnosis and avoid surgery. The patient responded to triple antituberculous therapy.

BCG Vaccine↗
PubMed1990

Insertional inactivation and cloning of the wA gene of Aspergillus nidulans.

We describe examples of wA gene inactivation (resulting in white conidiospores) obtained during transformation of Aspergillus nidulans. One wA- transformant was obtained by transformation with a prn+ plasmid of a strain with green conidia (wA+) which was unable to catabolize L-proline (prn-). This transformant contains a very large number of plasmid copies integrated at a single site inseparable from the wA locus. Passage of this transformant through the sexual cycle generated a variety of novel phenotypes for L-proline utilization, the number and frequency of which depended upon the cleistothecium from which the progeny were obtained, suggesting that the altered phenotypes were due to premeiotic events. The most extreme phenotype was severe hypersensitivity to L-proline. Hypersensitive progeny had a much reduced number of integrated plasmid copies enabling us to identify and clone putative prn-wA fusion sequences and subsequently retrieve wA sequences from a wild-type gene library. One of the wild-type clones overlapped the different sites of the insertional mutations in two wA- transformants and complemented the wA3 allele. Sequences within this clone hybridized to a transcript that was developmentally regulated in the wild type and absent in a number of mutants defective in conidiospore development. A reiterated sequence was also found in the region of the wA gene.

Aspergillus nidulans↗
PubMed1989

Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum.

An heterologous transformation system for the phytopathogenic fungus Fusarium oxysporum has been developed based on the use of the Aspergillus nidulans nitrate reductase gene (niaD). F. oxysporum nia- mutants were easily selected by chlorate resistance. The A. nidulans niaD gene was isolated from a gene library by complementation of an A. nidulans niaD mutant. The cloned gene is capable of transforming F. oxysporum nia- mutants at a frequency of up to ten transformants per microgram of DNA. Southern analysis of the DNA of the F. oxysporum transformants showed that transformation resulted in integration of one or more copies of the vector DNA into the genome.

Aspergillus nidulans↗
PubMed1988

Lectins as markers of endothelial cells: comparative study between human and animal cells.

Vascular endothelial cells were labelled with 10 vegetal lectins and 3 more monoclonal antibodies antiblood group ABO substances, in major organs of 14 common laboratory animals. After fixation in PLPa and paraffin embedding, cells were examined to determine their likeness to human cells. The most interesting reactive used was EEA, whose positivity defines upper mammalians. Blood B substance positivity and CSA negativity defines primates among which man is unique and defined by UEA I positivity and variability in ABO substance. CSA positivity defines non-primate upper mammalians. Rodents and birds were negative with all reactives tested. From the histochemical point of view, the animals closest to humans are monkeys, followed by swine and oxen, then by cat and dog and lastly by sheep. Rodents appear unrelated to humans in this system.

Animals↗
PubMed1987

Evonymus europaeus agglutinin as a marker of endothelial cells in the human.

Vascular endothelial cells were marked with Evonymus europaeus agglutinin (EEA) in the major organs of 6 subjects to reveal the anatomical distribution of positive elements in the human after fixation in paraformaldehyde-lysine-periodate and embedding in paraffin. EEA may be used as a marker of human adult vascular endothelium in the same way as Ulex europaeus Agglutinin 1, revealing the same positive endothelial and epithelial territories. The intensity of labeling was constant in a given subject of the B blood group. In A or 0 individuals the cerebral endothelium showed a much lower reactivity than the cardiac endothelium. This lectin, equally active in higher mammals, offers the unique advantage of direct comparison between species.

Adult↗
PubMed1987

[Labeling of testicular biopsies with lectins. Value of Evonymus europaeus agglutinin].

EEA was used to label testicular biopsies with 11 other lectins and three monoclonal antibodies anti blood group A, B, H substances. Although EEA's receptor chemical structure is still unknown and an electron microscopic control necessary, it is possible to use this lectin to label proacrosomic vesicles of spermatids in man and animal, allowing useful comparisons between species. The intensity of the reaction makes it interesting in the standard study, even automatized, of every testicular biopsy.

ABO Blood-Group System↗
PubMed1986

[Technical parameters influencing the reactivity of the vascular endothelium in relation to lectin of Evonymus europaeus].

Some parameters characterizing sample preparation for coloration with lectins were investigated in the model of sheep myocardial capillaries, revealed by EEA after formol fixation and paraffin embedding. The most influential factors are: addition of lysine-periodate to paraformaldehyde, duration of fixative immersion, oldness of paraformaldehyde dilution, delay of fixation (if below 8 h). All of them happen before embedding. On the other hand, those happening later are out of influence.

Animals↗
PubMed1985

[Labelling endothelial cells with the lectins from Cytisus sessilifolius and Ulex europaeus; comparison between human and animal cells].

Cytisus sessilifolius Agglutinin (CSA) was compared with Ulex europaeus Agglutinin (UEA1) for labelling endothelial cells fixed and embedded in paraffin. Human profile characterized by a dimorphism shown by UEA1 with a positive preponderant population and a minor negative one is never found in tested animals. CSA does not mark any endothelial cell in man but reveals endothelial cells in swine, sheep, ox, dog. A dimorphism exists in ox with the same repartition as the one shown in man by UEA1.

Animals↗
PubMed1985

[Labelling animal vascular endothelial cells with Evonymus europaeus lectin].

Evonymus europaeus Agglutinin (EEA) marks some vascular endothelial cells in animal as in man. Bindings is identical in organs frezen or fixed by paraformaldehyde-lysine-periodate and embedded in paraffin. Monkey, swine, ox, sheep, dog, have positive cells which are not shown in rabbit, rat, mouse or hen. But monkey is the only animal to show an endothelial dimorphism with a topography analogous to the one revealed by UEA1 in man.

Animals↗