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Biomedical subjects

F Rinaldi

Publications and source records attributed to F Rinaldi.

72 records · Page 4Linked to original sources

Biochemical purification of monkey and human LDH isoenzyme 5 suitable as immunization antigen.

A method consisting in an ion-exchange chromatography on DEAE-Sephadex A50 and an affinity chromatography on Oxamate-hexyl-Sepharose for the purification of monkey and human LDH isoenzyme 5 is presented. Analytical isoelectric focusing reveals minor variants in the monkey LDH isoenzyme 5. This material has been used to produce an antiserum from rabbits which recognizes all the human LDH isoenzymes containing the M subunit, without cross-reacting with LDH1.

Animals↗

Purification of human prostatic acid phosphatase and preparation of a specific antiserum in the rabbit.

A modification of previously described methods for the purification of human prostatic acid phosphatase from prostatic tissue is proposed. The purification scheme comprises an Ammonium sulphate fractionation and affinity chromatography on Concanavalin A-Sepharose after dialysis against water. After DEAE-Sephadex A 50 chromatography, the final step is an Ultrogel AcA 34 fractionation. The purified PAP has a specific enzymatic activity of 675 U/mg protein and the total yield is of 32%.

Acid Phosphatase↗