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Biomedical subjects

F Reyes

Publications and source records attributed to F Reyes.

At least 127 records · Page 7Linked to original sources
PubMed1982

Unusual intracytoplasmic immunoglobulin inclusions in chronic lymphocytic leukaemia.

Unusual intracytoplasmic immunoglobulin inclusions were found by immunofluorescence in three patients with chronic lymphocytic leukaemia. The inclusions contained the same immunoglobulin chains as those detected on the plasma membrane, except for delta chains which were expressed on the cell surface and not in the cytoplasmic inclusions. The cytoplasmic staining persisted throughout culture for 8 or more days. An initial study of patients 1's cells showed that the inclusions contained only mu chains, and kappa chains gradually became apparent after in vitro culture. In a second study, the fresh lymphocytes contained both mu and and kappa chains. Initially, biosynthetic experiments showed production of mu chains which polymerized in the cytoplasm and were not secreted. Subsequently there was synthesis of heavy and light chains which assembled into monomeric subunits that were retained and secretion of free light chains. The apparent molecular weight of these immunoglobulin chains was larger than that of their secretory counterparts. Immunoelectronmicroscopy revealed cytoplasmic mu chains in strands of endoplasmic reticulum. In the two other patients, immunofluorescence displayed unusual staining patterns of bright networks in perinuclear areas.

Cytoplasm↗
PubMed1982

Detection of surface immunoglobulins of human lymphoid cells: a comparative study of live and fixed cells using a direct immunoperoxidase procedure.

Surface immunoglobulins (Ig) of normal and malignant lymphoid cells were detected on prefixed, smeared (method A) and live (method B) cell suspensions; the results were compared with regard to staining patterns, specificity and sensitivity. In both methods surface Ig were detected by a direct immunoperoxidase procedure using conjugated purified antibody. Although method A has practical advantages, method B is more sensitive. The reasons for this discrepancy are discussed in relation to surface Ig denaturation and redistribution.

B-Lymphocytes↗
PubMed1982

The ultrastructural localization of immunoglobulins in human b cells of immunoproliferative diseases.

The cellular distribution of immunoglobulins in human malignant and normal B cells was investigated by immunoelectron microscopy by direct incubation of fixed cells with electron microscopy by direct incubation of fixed cells with peroxidase-coupled antibody. These conjugates penetrated into the cell, resulting in the simultaneous detection of surface and cytoplasmic immunoglobulins. The latter were seen as specific intracisternal staining of the perinuclear space and endoplasmic reticulum and occasionally of the Golgi complex. Plasma cells were frequently characterized by a heterogeneity of reactivity of the endoplasmic reticulum. Minute amounts of cytoplasmic immunoglobulin were demonstrated in cells without developed secretory organelles, such as lymphoma cells and lymphocytes from chronic lymphocytic leukemia (CLL). The method allowed us to define several subsets of cells according to the expression of surface and cytoplasmic immunoglobulins and thus to determine the stage of maturation of cells involved in monoclonal proliferation.

B-Lymphocytes↗
PubMed1982

[Diagnostic value of computed tomography in blood diseases (author's transl)].

Study of a series of 108 patients with blood disease explored by computer tomography showed that this is a reliable method for investigating retroperitoneal lymph node lesions, and that the cost and irradiation level was equivalent to conventional radiological examination. The scanner can also explore the liver, spleen, and lymph node chains, not revealed by the latter. Computer tomography, which is easily accepted by the patient, can be proposed, therefore, for initial and follow-up examinations of these patients.

Hematologic Diseases↗
PubMed1981

Acute myelodysplasia with myelofibrosis: a report of eight cases.

Eight patients with acute myelodysplasia and myelofibrosis are described. Four cases were secondary to long-term therapy with cytotoxic agents and four were idiopathic. All cases presented with an abrupt onset of the illness, absence of organomegaly and severe pancytopenia. Bone marrow aspirate yielded adequate material in four cases and showed myelodysplasic features. Study of histological sections indicated that the bone marrow was cellular in every case, including numerous dystrophic megakaryocytes, erythroblasts, immature cells of the granulocytic series and blast cells which were difficult to identify. The reticulin network was always increased. In each case the disease was rapidly fatal. No improvement was noted with chemotherapy. In three cases an overt leukaemia developed with marked pleomorphism of blast cells. The nosology of this syndrome is discussed.

Adult↗
PubMed1981

Detection of J chain in lymphomas and related disorders.

Lymph node specimens from 125 patients with malignant lymphomas and related disorders were studied by immunoperoxidase procedure for the presence of intracytoplasmic immunoglobulin (CIg) and J chain CIg staining was present in 22/24 cases of lymphoplasmacytic-lymphoplasmacytoid lymphomas, and in 10/10 cases of extramedullary plasmacytomas and myelomas. In the majority of these cases J chain could be demonstrated in plasmacytoid or neoplastic plasma cells. In 21/36 cases of immunoblastic lymphomas, intracytoplasmic Ig staining was present. In only two of the 36 cases were the lymphomatous cells stained positively for J chain. J chain was not detected in other lymphomas such as lymphocytic lymphomas, follicular lymphomas, lymphoblastic lymphomas or in Reed-Sternberg cells or hairy cells. J chain was demonstrated in mature plasma cells and immunoblastic cells in hyperplastic lymph nodes, and in angioimmunoblastic lymphadenopathy. These findings show that J chain is not detectable in all B cell lymphomas even in the presence of CIg synthesis, irrespective of class.

Cytoplasm↗
PubMed1980

Immunoperoxidase detection of immunoglobulins in cells of immunoproliferative diseases. A comparison between conjugate and nonconjugate (PAP) procedures.

Two immunoperoxidase procedures, direct conjugate and peroxidase-antiperoxidase (PAP) nonconjugate, were compared by studying surface and cytoplasmic immunoglobulins in identical smeared or embedded material from patients having various conditions involving B-cell proliferation. The direct procedure was carried out with affinity-purified antibodies; the PAP procedure was carried out with commercially available antisera and in some experiments by also using purified antibodies as a primary layer. This study confirms the feasibility of both procedures for staining human B cells, although surface immunoglobulins were not visualized in tissue sections. By comparing the phenotypes obtained with both procedures, this study also emphasizes the need for highly specific reagents and the possible shortcoming represented by contaminating specificities present in the first serum layer of the PAP procedure. Diluting primary antiserum was effective in eliminating such unwanted specificities, but at the same time could alter the genuine phenotype of cells. This study emphasizes the need for highly specific reagents such as solid-phase immunoadsorbed antibodies.

Burkitt Lymphoma↗
PubMed1980

Acute non myeloid leukaemia in adults. Prognostic factors in 92 patients.

An analysis of prognostic factors was performed on a series of 92 adult patients with acute non-myeloid leukaemia (ANML). Four factors were shown to be correlated with a poor short-term prognosis: (1) Neuropathy involving a single cranial nerve (numbness of the chin) as a presenting feature. (2) A low percentage blast cell infiltration of bone marrow (less than 55%). (3) Cytological L2 type or L3 type of cells according to FAB classification proposals. (4) Increased age. Two factors were shown to be correlated with a poor long-term prognosis: 1) A high level of circulating blast cells; 2) A high tumour burden, as in childhood ANML. These two long-term prognostic factors were interrelated. In addition, our results suggested that myelofibrosis could be a poor long-term prognosis factor as well.

Acute Disease↗
PubMed1980

Lytic enzymes in the autolysis of Schizophyllum commune with special reference to 1,3-alpha-glucanase.

In the autolytic phase of growth Schizophyllum commune lost 62% of its dry weight in 70 days of incubation. The variations in the activity of some lytic enzymes were studied in the culture fluid and mycelial extracts during growth and autolysis of this fungus. The enzymes 1,3-beta-glucanase (exoglucanase), 1,3(4)-beta-glucanase (endoglucanase), alpha-amylase, and invertase behaved in the same way in culture fluid and mycelial extract, but their activities were much higher in the culture fluid. The enzyme activities increased during autolysis, but then decreased at the end of this period except in the case of alpha-amylase which remained high. It was only possible to detect 1,6-beta-glucanase, cellulase, and polygalacturonase activities at certain times during the autolytic phase of growth. The enzyme chitinase was not detected and 1,3-alpha-glucanase (S-glucanase) occurred in the mycelial extract at a higher concentration than in the culture fluid. A decrease in the activity of this enzyme in the mycelial extract and an increase in the culture fluid occurred during autolysis. The enzyme 1,3-alpha-glucanase exhibited two optima pH, one at 6.0 and the other at 8.0. The Km value for the latter was 0.02 M at pH 5.5 in borate-citrate-phosphate buffer.

Agaricales↗
PubMed1980

[Comments about use of polychemotherapy MOPP associated with radiotherapy in the treatment of early stages of Hodgkin's disease. Preliminary report of 58 patients (author's transl)].

58 patients with early stages of Hodgkin's disease (I, II, III l) were treated by 3 courses of MOPP chemotherapy followed by extended field irradiation. After 31 to 72 months follow up 87,8 p. 100 of patients are alive in complete remission. The study of the group deserves two comments: 1. Adjuvant MOPP polychemotherapy may be useful, only when a complete response is obtained after 3 courses, when it is so a complete remission of long duration may be hoped in nearly 100 p. 100 of cases. 2 After complete remission induced by combined chemotherapy and radiotherapy, maintenance chemotherapy seems to be useless.

Adolescent↗