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Biomedical subjects

F Pinet

Publications and source records attributed to F Pinet.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Characterization of the enzyme involved in the processing of big endothelin-1 in human lung epithelial cells.

Biosynthesis of active endothelin-1 (ET-1) implies an enzymatic processing of the inactive precursor Big ET-1 (1-39) into the mature, 21 amino acid peptide. The aim of this study was to characterize in airway and alveolar epithelial cells the enzymes responsible for this activation. BEAS-2B and A549 cells, which both produce ET-1, were studied in vitro as models for bronchiolar and alveolar cells, respectively. Both cell lines were able to convert exogenously added Big ET-1 (0.1 microM) into ET-1, suggesting a cell surface or an extracellular processing. The conversion was inhibited by phosphoramidon in both cell lines with an IC50 approximately 1 microM, but not by thiorphan, a specific inhibitor of neutral endopeptidase 24.11 (NEP). The endogenous production of serum-stimulated BEAS-2B and A549 cells was not inhibited by thiorphan, and phosphoramidon showed inhibition only at high concentration (>100 microM). Western blotting following electrophoresis in reducing conditions demonstrated a protein of MR 110 corresponding to the ECE-1 monomer in both BEAS-2B and A549 cells, as well as in whole lung extracts. By RT-PCR we revealed the mRNA encoding for the ECE-1b and/or -1c subtype, but not ECE-1a, in both cell lines. We conclude that BEAS-2B and A549 cells are able to process either endogenous or exogenous Big ET-1 by ECE-1 and that isoforms 1b and 1c could be involved in this processing with no significant role of NEP.

Cell Line↗
PubMedDate not supplied

Emergency selective aortography for acute lesions of the thoracic aorta.

Emergency selective aortography is necessary for the definitive diagnosis of acute lesions of the thoracic aorta and defines their therapeutic possibilities. Cineradiography is very helpful and is complementary fo the roentgenograms. In this paper the study of dissecting aneurysms, tears of the aortic wall, ruptured aortic aneurysms and traumatic aortic lacerations is described. A new anatomic classification of dissecting aneurysms combined with that of De Bakey et al. is presented.

Aorta, Thoracic↗
PubMedDate not supplied

Regulation of human renin gene transcription by cAMP.

To delineate the cis-acting elements of the proximal promoter responsible for cAMP-induced human renin gene transcription, 5'-flanking regions of the human renin gene were fused to a luciferase reporter gene and transfected in chorionic cells. Forskolin treatment induced the expression of luciferase by 2.4 fold when the reporter plasmid contained the promoter region (-582 to +16). Mutation or deletion of the CRE diminished (1.7 fold) but did not abolish cAMP-induced transcription, demonstrating that region containing the CRE and region containing a Pit-1 site were both necessary for cAMP maximal induction. Taken together, these results show that the cAMP response of the human renin gene may involve CREB binding the CRE and tissue-specific factors (from chorionic and kidney cell origin), different from Pit-1, that interact with the Pit-1 response DNA elements.

Base Sequence↗
PubMedDate not supplied

Cine-angiography during percutaneous transluminal angioplasty of the lower limbs.

In the treatment with percutaneous transluminal angioplasty of 65 atheromatous stenosis of the lower limbs including 50 iliac and 15 femoro-popliteal lesions, the authors insist on the value of cine-angiography during the dilatation. Cine-angiography increases the security and the velocity of the examination. The anatomic data of this technic are as precise as those obtained with seriography and a special hemodynamic investigation is obtained with the direction of the flow of the contrast medium in the collateral branches and in the main artery. The cost is less expansive than with seriography.

Angioplasty, Balloon↗
PubMedDate not supplied

[Primary tumors of the diaphragm. Apropos of a case].

The diagnosis of diaphragmatic tumours is complicated by their rarity and because they are often difficult to distinguish from the more frequent tumours of surrounding structures. We describe two cases: an invasive fibroma and a primary hydatid cyst. We discuss the differential diagnosis of diaphragmatic tumours and suggest a systematic approach to their radiological diagnosis, stressing the value of ultrasonography, CT scanning and diagnostic pneumoperitoneum.

Aged↗