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Biomedical subjects

F Penninckx

Publications and source records attributed to F Penninckx.

At least 109 records · Page 6Linked to original sources
PubMed1988

Changes in small intestinal epithelial expression of MHC class II antigen after terminal ileal resection for Crohn's disease.

Aphthous lesions in the neoterminal ileum from patients operated for Crohn's disease are an early sign of recurrence that can be identified during ileocolonoscopy. The origin of these lesions was studied in nine patients treated by terminal ileal resection and right hemicolectomy for complicated Crohn's disease. During surgery the neoterminal ileum was turned inside out, the mucosa was carefully inspected and two large mucosal biopsies were obtained. The same procedure was carried out in seven patients operated for other diseases. Four to six months after surgery endoscopy of the neoterminal ileum was carried out and multiple biopsies were obtained from the neoterminal ileum. Another follow-up colonoscopy with biopsies was carried out one year after the operation. The operative specimens and the per- and postoperative biopsies were submitted to routine microscopy and immuno- and enzyme-histochemistry. None of the Crohn's patients had macroscopic lesions in the neoterminal ileum at operation and only one had microscopic signs of inflammation and a positive section margin. Four-six months after operation all Crohn's patients had active aphthous lesions in a 5-20 cm segment of the neoterminal ileum at endoscopy. Biopsies taken at this time showed microscopic features which were not observed in biopsies from control subjects: an increase of HLA-DR+, ATPase+ dendritic cells in the ileal mucosa and a defective expression of MHC class II antigens by the small intestinal epithelial cells. MHC class II expression by the small intestinal epithelial cells returned towards normal after one year.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Triphosphatases↗
PubMed1987

The use of biochemical parameters for a qualitative and quantitative assessment of ischemic damage to the small-intestinal mucosa.

Lactate dehydrogenase has been measured in the small-intestinal mucosa in order to assess its value as a marker for the effects of ischemia and of reperfusion. The decrease in specific activity of the enzyme illustrates the deleterious effect of reperfusion on the quality of the remaining epithelial cells. However, this parameter fails to detect the loss of epithelial cells, which is the major event during ischemia as well as during reperfusion. In contrast, the expression of enzyme activity per g protein of the underlying intestinal muscle allowed us, in addition, to assess quantitatively the loss of epithelial cells, in good agreement with the histological data.

Animals↗
PubMed1987

Anal dilatation.

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Adult↗
PubMed1987

Transection of the pancreas demonstrated by ultrasound and computed tomography.

The early occurrence of peritoneal signs and hyperamylasemia in a 14-year-old boy, who had fallen off his horse, urged us to perform a sonographic and computed tomographic study of the upper abdomen. Both examinations showed a complete pancreatic rupture. Distal pancreatectomy led to a rapid and uneventful recovery.

Adolescent↗
PubMed1987

Hepatic echinococcosis ruptured into the biliary tract. Clinical, radiological and therapeutic features during five episodes of spontaneous biliary rupture in three patients with hepatic hydatidosis.

Three patients are described with hepatic involvement by Echinococcus granulosus, complicated by spontaneous rupture into the biliary tract. Clinical features consisted of upper abdominal pain, jaundice, fever, anorexia, and vomiting. Hepatomegaly and marked epigastric tenderness were consistently observed. Laboratory findings included obstructive liver function tests, leucocytosis, eosinophilia, and hyperamylasemia. Abdominal computed tomography, showing the cystic wall, the presence of wall calcifications, daughter cysts and wall enhancement, provided a correct diagnosis of hepatic hydatidosis in all patients. Dilatation of the bile ducts with the presence of intraluminal material was clearly shown by sonography and endoscopic retrograde cholangiography. These abnormalities were most frequently found in the common bile duct and in the left hepatic duct. On sonography, the intraluminal material appeared as amorphous, sludge-like hydatid sand, and as daughter cysts. On ERCP, the intrabiliary parasitic material appeared as non-homogeneous, irregularly shaped and mobile filling defects. Other findings at ERCP were displacement and distortion of intrahepatic bile ducts by the hepatic cysts and a mild dilatation of the pancreatic duct. In one occasion, evacuation of a daughter cyst through the papilla was observed. The therapeutic value of mebendazole and endoscopic sphincterotomy in our patients is discussed.

Adult↗
PubMed1987

Biochemical and functional aspects of recovery of mammalian systems from deep sub-zero temperatures.

The viability of isolated mammalian systems is, apart from possible morphological changes, essentially conditioned by the biochemical modifications from normal physiological conditions to an artificial environment where blood supply is interrupted leading to ischaemia and where the temperature is lowered. In order to survive freezing and thawing, mammalian systems have to be protected by cryoprotectants, which apart from some inherent toxicity, may also interact with vital metabolic mechanisms (Conover, 1969, 1975: Fahy, 1986: Fahy et al. 1984: Jacobs & Herschler, 1986: Karow, 1982: Penninckx et al. 198 3: Polge et al. 1949: Rowe et al. 1980: Schlafer, 1981: Taylor & Pignat, 1982). Cellular volume changes as a result of modifications in extra- a and intracellular osmolality occurring during freezing and thawing prove particularly detrimental to the normal functioning of the cellular membranes (Crowe et al. 1983: Farrant, 1980: Farrant et al. 1977b: Karow, 198 2: Mazur & Rigopoulos, 1983: Meryman, 1970: Meryman et al. 1977: Nei, 19 76: Santarius & Giersch, 1983). Furthermore intracellular ice formation enhances structural and metabolic injury to subcellular particles(Farrant et al. 1977a: Fink, 1986: Fishb ein & Griffin, 1976: Fujikawa, 1981: Fuller & De Loecker, 1985: Lazarus et al. 1982: Malinin, 1972: Mazur, 1984: Pavlock et al. 1984:Penninckx et al. 1984: Persidsky & Ellet, 1971: Rubinacci et al. 1986: Shikama, 1965: Steponkus & Wiest, 1979: Strauss & Ingenito, 1980: Takehara & Rowe, 1971: Tamiya et al. 1985). Even with the protection of structural integrity, the preservation of energy production and the maintenance of the specific intracellular medium are essential to secure viability (Pegg, 1981).

Animals↗
PubMed1986

The cellular composition of granulomas in mesenteric lymph nodes from patients with Crohn's disease.

The immunohistochemical findings in granulomatous lymphadenitis in patients with Crohn's disease are presented and compared with conventional light microscopic findings. The cellular composition of the granulomas in mesenteric lymph nodes was examined with a broad panel of monoclonal and polyclonal antibodies directed to B-cells, T-cells, monocytes/macrophages, dendritic reticulum cells, HLA-DR antigens and the transferrin receptor. The centre of the granulomas contains OKIa+, OKM1+, OKT9+, DRC-, To5- epithelioid cells and giant cells and OKT3+ lymphocytes. In general, the majority of the small lymphocytes within the granulomas expresses the OKT3+, OKT4+ Leu 3a+ phenotype which points toward T-helper cells. Fewer OKT3+ OKT8+ T suppressor/cytotoxic cells are observed. At the periphery of the granulomas a lymphocytic corona composed of BA1+, B1+ B lymphocytes may be present. From these findings it can be concluded that the granulomas in mesenteric lymph nodes from patients with Crohn's disease are composed of centrally located T-lymphocytes and of epithelioid cells which are of monocyte/macrophage origin and have the characteristics of antigen-presenting cells.

Adolescent↗
PubMed1986

Internal sphincter-saving in imperforate anus with or without fistula. A manometric study.

Manometric assessment was performed in 54 infants with congenital ano-rectal anomalies including those with a blindly ending rectum (6 cases) or with an ectopic bowel outlet either in the perineum (25 cases), vulva (8 cases), vagina (8 cases), urethra (5 cases) or bladder (2 cases). Infants with an externally accessible orifice were investigated preoperatively whereas those with an internal orifice or no orifice could only be evaluated after surgical correction. In all operated cases presented, the ectopic rectal opening was preserved together with its surrounding smooth musculature. It was transplanted ventral to the puborectalis sling into the anal dimple if present. A normal functioning internal sphincter was observed in all infants even after transplantation. Anal resting tone was normal in all cases with mean values in the various subgroups ranging from 32 +/- 10 mmHg to 51 +/- 6 mm Hg. Frequency of anal slow pressure wave activity ranged from 11.6 +/- 1.5/min to 15.7 +/- 1.3/min. The recto-anal inhibitory reflex was present in all cases. The term fistula should be reserved for morphologically and physiologically abnormal microscopic communications. Failure to appreciate the presence of a normal internal sphincter may account for poor functional results after surgery. The results demonstrate the importance of preserving the internal sphincter in surgical correction of anorectal anomalies.

Anal Canal↗
PubMed1986

Preservation of taenia coli by freezing and storage at -196 degrees C.

Some damaging effects that occur during cryopreservation by freezing to -196 degrees C have been evaluated in rabbit taenia coli by analyzing the proportional recovery of acetylcholine- and histamine-induced maximal contractions. Dimethyl sulfoxide (Me2SO) 10 v/v% was used as the cryoprotectant; it reversibly abolishes spontaneous contractility even after incubation at 37 degrees C during 2 hr. Programmed freezing at 0.6 degrees C/min with compensation for the latent heat of fusion and warming at 35 degrees C/min proved to be slightly superior to programmed cooling without compensation and slower warming. The degree of functional recovery was comparable after either abrupt or stepwise removal of Me2SO. Freeze-thawing resulted in a significant reduction of contractile force in each buffer solution tested, and acetylcholine-induced contractility was always better preserved than histamine-induced contractility. The best preservation (approximately 65%) was obtained in a potassium-rich buffer solution. The absence of calcium and magnesium from the incubating medium had no influence, whereas the presence of EDTA significantly affected functional recovery. It is difficult to compare our results with those reported by others because of multiple methodological differences. However, it seems that previous results can be improved by changing the freezing rate and the composition of the incubating and cryoprotecting medium.

Acetylcholine↗
PubMed1984

Erythrocyte swelling after rapid dilution of cryoprotectants and its prevention.

These in vitro studies on canine red blood cells confirm that cell swelling occurs after rapid dilution of Me2SO and glycerol. Cells loaded with a penetrating cryoprotectant in a medium with low Na+, high K+ composition present significantly less swelling after rapid dilution of the cryoprotectant than cells exposed to an electrolyte medium characterized by high Na+, low K+ composition. The osmotic cell stress during rapid dilution of Me2SO can be completely counteracted by the simultaneous use of the nonpenetrating sorbitol during exposure and loading. However, the addition of sorbitol is of no important benefit when glycerol is used as the intracellular cryoprotectant. This is probably due to the slower elution of glycerol. Thus utilizing a perfusion solution containing sorbitol during loading and dilution of Me2SO reduces the osmotic injury and may greatly improve the survival prospects of cryopreserved organs by avoiding "out-flow" block.

Animals↗
PubMed1984

Primary gastric actinomycosis: a case report and review of the literature.

A case of primary gastric actinomycosis presenting as a bleeding gastric tumor is reported. The diagnosis of this infection of the stomach is difficult and may often be established only by histological examination. If the disease is recognized, the prognosis is good because antibiotic treatment is very effective. Recent literature findings are reviewed and clinical and endoscopic features are compared.

Actinomycosis↗
PubMed1983

The effects of different concentrations of glycerol and dimethylsulfoxide on the metabolic activities of kidney slices.

Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure to glycerol by incubation of kidney slices in Krebs-Ringer bicarbonate buffer containing varying concentrations of glycerol from 0 to 70% (v/v) resulted in a pronounced inhibitory effect on the protein synthesizing activity, while thymidine incorporation into DNA and the alpha-aminoisobutyric acid uptake through the cell membranes were less affected. Exposure of the tissue to buffer containing dimethylsulfoxide (Me2SO) in concentrations of 10 to 20% (v/v) resulted in a stimulatory effect on metabolism. At higher concentrations, Me2SO was toxic resulting in damaging effects on protein and DNA synthesis as well as on membrane integrity. The stimulatory effects of exposure to low concentrations of Me2SO on protein and DNA synthesis in kidney slices were concluded to be the result of an increased transport of precursors through the cell membranes.

Animals↗
PubMed1980

Pre-operative and post-operative evaluation of the rectal bladder.

Five Heitz-Boyer-Hovelacque operations in children were reviewed in which particular attention was paid to pre-operative examinations, including electromanometry (EMM) and post-operative evaluation of continence, functional result and renal function. In our limited experience the rectal bladder operation is a good procedure in selected cases; it produces satisfactory continence and maintains renal function.

Adolescent↗