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Biomedical subjects

F Pasmans

Publications and source records attributed to F Pasmans.

25 records · Page 2Linked to original sources
PubMed2003

First report on Cryptococcus laurentii associated with feather loss in a glossy starling (Lamprotornis chalybaeus).

Although Cryptococcus laurentii has been isolated from fresh droppings and cloaca samples from feral pigeons, it has never before been associated with an actual disease condition in birds. This case study deals with the first report on C. laurentii associated with feather loss in a glossy starling (Lamprotornis chalybaeus). The bird exhibited patchy feather loss, especially around the back and beak area, and greyish crusts sticking quite firmly to the underlying skin. The feathers had a greasy appearance and disseminated a musty odour. Treatment was installed with fluconazole in the drinking water. One month following the onset of treatment, the condition of the plumage had markedly improved.

Animals↗
PubMed2002

Interactions of Salmonella enterica serovar Muenchen with macrophages of the turtle Trachemys scripta scripta.

Interactions of Salmonella with macrophages have been studied in birds and, most extensively, in mammals. In these homeothermic animals, interactions between Salmonella and macrophages are characterized by the following processes. After macropinocytosis, spacious phagosomes are formed within the macrophage. Partial inhibition of phagosome-lysosome fusion and resistance to the formation of reactive oxygen species and reactive nitrogen intermediates enable the bacterium to survive and even multiply within the host macrophage. Eventually, Salmonella will induce apoptosis of the macrophage. In this study, interactions of peritoneal macrophages of the turtle Trachemys scripta scripta with Salmonella enterica serovar Muenchen were examined in vitro. Turtle macrophages were able to phagocytise Salmonella efficiently at both 30 and 37 degrees C. Exposure of macrophages to Salmonella induced the production of reactive oxygen species, which could be partially suppressed by adding the NADPH oxidase inhibitor diphenylene iodonium. Initially, most of the intracellular bacteria were killed. However, Salmonella proved to be able to persist and multiply inside turtle macrophages at both 30 and 37 degrees C for at least 48 h, despite the production of reactive nitrogen intermediates by inducible NO synthase. Salmonella infection of turtle macrophages killed the phagocytes at both 30 and 37 degrees C. These findings demonstrate that no obvious qualitative differences exist between macrophage-Salmonella interactions from homeothermic animals and from turtles. This indicates that other factors are responsible for the different course of Salmonella infections in homeothermic and poikilothermic hosts.

Animals↗
PubMed2001

Induction of the respiratory burst in turtle peritoneal macrophages by Salmonella muenchen.

Peritoneal macrophages were collected from juvenile turtles 72h after intraperitoneal inoculation with a 3% Sephadex suspension. The macrophages were assayed for their chemiluminescent (CL) properties, reflecting their respiratory burst activity, after stimulation with Zymosan A, phorbol 12-myristate 13-acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (fMLP), and calcium ionophore A23187. Except for fMLP, all triggering agents induced a marked CL response. Luminol was used as the chemiluminescent probe. When comparing CL responses in temperatures ranging from 15 to 35 degrees C, lower assay temperatures induced lower and slower CL responses. Stimulation with viable Salmonella muenchen resulted in a distinct response. Bacteria, inactivated by means of heat or acetone, induced a faster and stronger oxidative burst. Opsonization of either viable or heat-inactivated S. muenchen with non-inactivated anti-S. muenchen serum, prepared in turtles, induced faster and higher CL responses. On the other hand, opsonization of acetone-inactivated S. muenchen caused CL responses to be slower and weaker. S. muenchen, opsonized with heat-inactivated turtle anti S. muenchen serum, induced higher responses than non-opsonized bacteria, but slower and weaker responses than bacteria opsonized with native turtle antiserum. No response was recorded after stimulation with LPS and the supernatant of heat-inactivated bacteria.

Acetone↗
PubMedDate not supplied

Interactions of Salmonella enterica subsp. enterica serovar Muenchen with intestinal explants of the turtle Trachemys scripta scripta.

Salmonella infections in reptiles, in contrast to those in birds and mammals, are limited to the intestinal tract. In this study, interactions of a strain of Salmonella enterica subsp. enterica serovar Muenchen (SEEM) with intestinal explants of the turtle Trachemys scripta scripta were examined by scanning electron microscopy (SEM). Adhesion and invasion in the chelonian intestinal explants at 30 degrees C and 37 degrees C were evaluated quantitatively. For purposes of comparison, the invasive capacity of SEEM in the continuous avian epithelial cell line DIV-1 at 30 degrees C and 37 degrees C was determined. Small numbers of M-like cells were found in the ileum of the turtles. The bacteria adhered mainly to the mucus of the intestinal explants. Only small numbers of salmonellae were associated with epithelial cells. Higher numbers of bacteria adhered at 30 degrees C than at 37 degrees C. Epithelial damage, embedding of bacteria in the epithelial surface and a ruffling-like process were noted only at 37 degrees C. Minimal numbers of salmonellae invaded the explants at 30 degrees C and 37 degrees C. Invasion of DIV-1 cells was greater at 37 degrees C than at 30 degrees C. The study suggested that the intestinal mucous layer provides an important site of colonization for salmonellae in the chelonian host and protects the underlying epithelial cells.

Animal Diseases↗