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Biomedical subjects

F Papa

Publications and source records attributed to F Papa.

At least 55 records · Page 3Linked to original sources
PubMed1987

[Acetylcholinesterase titers in the amniotic fluid. Value of screening dysraphia of the central nervous system].

The authors report the results of 434 titrations of acetylcholinesterase in the amniotic fluid sampled by early amniocentesis, between the 17th and 24th week of amenorrhea. The biochemical technique used was quantitative and the values of the total cholinesterase activity (TchE) and the true acetylcholinesterase (AchE) were studied separately. Their conclusion is similar to that of other authors: the quantitative method, although satisfactory, has less value than the qualitative method using electrophoresis on polyacrylamide gel in order to screen or confirm the existence of a dysraphia of the central nervous system, because any amount of soiled fetal blood elevates the acetylcholinesterase level, titrated by the quantitative method.

Acetylcholinesterase↗
PubMed1983

[Determination of the acetylcholinesterase activities of the amniotic fluid on a centrifugal analyzer].

Amniotic fluid cholinesterase (ChE) activity measurements are realized to detect pre-natal neural tube defects. Their determinations on centrifugal analyzer are described. Total ChE activity is assayed at 412 nm with dithiobis-(nitrobenzoic-acid). Acetylcholinesterase activity is measured in the presence of ChE inhibitor, 14 X 10(-6)M lysivane; our results about the inhibitor concentration confirm the previous studies of Dale (Clin. Chim. Acta, 1977, 77, 407-413, Lancet, 1980, ii, 975) in opposition to those of Brock (Lancet, 1981, i, 95). Non NTD amniotic fluids from 92 women between the 17th and 20nd weeks of pregnancy were used to establish normal values. The technique proposed is rapid, accurate and needs only 40 microliters sample.

Acetylcholinesterase↗
PubMed1983

[Preconceptional selection of sex using the ionic method. Dietary regime. Results of a 2 years' prospective clinical study].

45 women out of a total of 58 patients who followed a dietary regime appropriate to the sex they had chosen for their babies were successful in delivering a baby of the wanted sex. This result gives a success figure of 77.6% (the mean between 67 and 89%: p less than 0.001) and from the statistical point of view this difference is significant as compared with the natural sex ratio. The diets were overall well tolerated and the children at birth weighed normal weights. The parents seemed to request approximately equal numbers of girls as of boys--a balanced request. A high number of patients abandoned the method (75%). This is explained by the restrictions that are imposed by the diets and because the method would still appear to be experimental. It does seem that the ability to influence the sex ratio of the future offspring is possible when the ionic balance of the diet is changed. The study should be continued.

Clinical Trials as Topic↗
PubMed1979

[Early amniocentesis, 1061 punctures and 1000 pregnancies].

The authors analysed 1061 early amniocentesis carried out during 1000 pregnancies. The indications were as follows: chromosomal abnormalities (79,6 p. 100), fetal karyotypes for X-linked diseases (4,7 p. 100), metabolic disorders (5,9 p. 100), amniotic fluid alpha-foetoprotein (9,8 p. 100) in neural tube defect or congenital nephrosis. Amniotic fluid was obtained on the first attempt in 98.2 p. 100 and on the second attempt in 100 p. 100. The fluid was heavily blood-stained in 1,7 p. 100, sanguinolent in 3,8 p. 100 and brownish in 2,40 p. 100. Cells were grown on the first attempt in 98 p. 100 and on the second attempt in 100 p. 100. The outcome of pregnancies was correlated with the indications of amniocentesis. The rate of spontaneous abortion is 1,7 p. 100, but only 6 of them can be due to amniocentesis (0,6 p. 100). Perinatal mortality was 1,8 p. 100: mortinatality (1,2 p. 100) and neonatal mortality (0.6 p. 100). All this fetal deaths have other causes. The fetal loss was 3,9 p. 100. Fetal morbidity was low: none fetal injuries, none cutaneous scar, few premature deliveries (1,6 p. 100), some malformations more or less serious (2,6 p. 100) with 4 congenital luxations of the hips (0,45 p 100). Maternal morbidity was limited at a greater cesarean section rate: 21 p. 100 (30 p. 100 in women 40 years old and more), none feto-maternal rhesus immunization was observed because immunoprophylaxis was strictly performed. The rate of therapeutic abortion was 4,6 p. 100 without any diagnostic error. Diagnostic accuracy was 100 p. 100. Several conditions are necessary to be the procedure safe accurate and reliable: appropriate genetic counseling, exact determination of gestational age (17 international weeks), sufficient volume of amniotic fluid counseling, exact determination of gestational age (17 international weeks), sufficient volume of amniotic fluid (uterus size: 12 cm), gynecologic examination by operator himself, perfect echography to localize the placenta and detect multiple gestations, adequately trained obstetrician, use of 20 gauge spinal needle, stric asepsis, experimented laboratory and experienced staff.

Amniocentesis↗
PubMed1978

[Early amniocentesis in twin pregnancies (author's transl)].

Since 1972, we have performed 951 early amniocentesis in 902 pregnant women. There were 19 twin pregnancies, all diamniotic, representing 2.1% of the sample. Diagnosis was always suspected before the amniocentesis. It was confirmed by echography in the 14 cases in which this technique was used. Out of the first 5 cases without echography, we punctured only one sac. In the 14 last cases, the process was successful in 13 cases thanks:--the perfection of echography showing the exact location of the fetuses and the position of the septum--the injection of congo red (12 cases) or methylene blue (2 cases) into the first sac. The indications were as follows: chromosomal aberrations (14 cases) metabolic diseases (1 case), sex-linked disorders (1 cases) neural tube defects (3 cases). No complications due to amniocentesis were observed but three abortions and one fetal death occurred. The reason why is discussed. We have made only one therapeutic abortion.

Abortion, Spontaneous↗