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Biomedical subjects

F Negretti

Publications and source records attributed to F Negretti.

36 records · Page 2Linked to original sources
PubMed1983

Immunomicrobiological monitoring of orovaccinal therapy in acute intestinal infections.

A double blind study in 42 patients (treated and controls) affected by acute uncomplicated salmonellosis was performed. The treatment consisted of an oral inactivated tetravaccine at the dose of 1 ampoule twice a day for 15 consecutive days. Microbiological and serological techniques (determination of intestinal secretory IgA by means of immunofluorescence, titration of copro and serum antibodies, quali-quantitative coprocultures) have shown that the administration of oral vaccine improved both the clinical and the immunomicrobiological picture in treated patients.

Acute Disease↗
PubMed1977

[Studies of the microbial content of raw materials used in pharmaceutical preparations].

The microbiological controls (determination of the total and mycetic bacterial count; isolation of enterococci, Clostridium, Pseudomonas aeruginosa, Staph. aureus, E. coli, Salmonella and other Enterobacteriaceae) executed on 1517 samples of 100 varous raw materials, have ascertained the presence of a microbial contamination varying to matters, with presence also of pathogenic or hygienically undesirable microrganisms. The more contaminated substances have resulted to be those organic either of vegetable or animal origin. Sensible differences have been noted between the various lots and according to the various suppliers. The conservation trials under various conditions of temperature (15-22-37 degrees C) and humidity (30 degrees - 60 degrees - 90 degrees of relative humidity) with opened or closed recipients, have demonstrated the possibility of remarkable increases of the number of the microrganisms in the raw materials with increase of temperature and humitidy, especially in the non hermatically closed recipients.

Bacteria↗
PubMedDate not supplied

Chromophobe renal cell carcinoma: clinicopathological study of 7 cases.

Chromophobe renal cell carcinoma (CRCC) may be grossly and microscopically confused with oncocytoma. It is now believed that many, if not all, of the so-called malignant oncocytomas or oncocytomas with metastases reported in the literature were indeed chromophobe renal cell carcinomas. CRCC is characteristically positive for colloidal iron and shows cytoplasmic microvesicles in electron microscopy. This study of CRCC is thought to be the first one done in Latin America. Of a total of 106 renal epithelial neoplasms, 7 (6.6%) fulfilled the criteria for chromophobe renal cell carcinoma. This frequency in Brazil is similar to that in other parts of the world. There was no difference in age, sex, and race distribution of CRCC compared to usual renal epithelial tumors. Grossly, the CRCC ranged in size from 3.5 to 20 cm (average: 10.2 cm) in greatest dimension. Most frequently, the tumor was brown on the cut surface. The growth pattern showed compact areas in all tumors and, in most of the cases, both clear and eosinophilic cellular subtypes were seen. The electron microscopic findings favor an origin of the microvesicles from outpouchings of the outer membrane of mitochondria. The strong positivity for colloidal iron in spite of the destruction of the cytoplasmic vesicles in paraffin-embedded specimens seems to indicate that the acid mucopolysaccharides are not located inside the microvesicles. By the time of diagnosis, only one case had regional lymph node metastases and this particular case was the only one mixed (associated with the usual renal cell carcinoma). The follow-up examination after nephrectomy showed that prognosis seems to be favorable in CRCC, except when the tumor coexists with the usual renal cell carcinoma.

Adenocarcinoma↗
PubMedDate not supplied

Microbiological control of pharmaceutical products containing preservatives: drawbacks of the method of membrane filtration.

Microbiological assays carried out on membranes employed for the filtration of solutions of preservatives with concentration used in pharmaceuticals, demonstrated that such membranes, even after washing with 300 ml (100 ml X 3) of peptone water according to the guidelines of the Pharmacopoeia, maintain very frequently a remarkable antimicrobial activity revealed by inhibition zones and by disappearance or decrease of the number of colonies forming units. Such drawbacks can interfere negatively with the microbiological controls of pharmaceuticals containing preservatives.

Drug Contamination↗
PubMedDate not supplied

[The sequence of specific immune reactions at the biliary level after oral vaccination].

The appearance and the progression of the specific immunoreactions at biliary levels (agglutinant titre, immunoenzimatic titration of IgAGM, IgA, IgG) in rabbits treated per os from 1 to 10 times, daily or bi-weekly, with 20 and 40 milliard of inactivated bacterial antigens (E. coli, Proteus vulgaris, Staph. aureus, Str. faecalis) have been studied. The appearance of the specific biliary antibodies has been very precocious (only one day after the first administration) and the values increased progressively with the number of administrations. The posology of 40 milliard and the bi-weekly sequence of the treatments presented the highest immunoreactions. In the bile the IgA and IgG increased considerably, but the IgA have been preponderant; in the serum only the IgG presented a remarkable increase, not the IgA, that are present in very small quantity.

Administration, Oral↗