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Biomedical subjects

F Moreno

Publications and source records attributed to F Moreno.

At least 289 records · Page 16Linked to original sources
PubMed1980

A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme beta-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a beta-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

Amino Acid Sequence↗
PubMed1980

Alterations of hepatic alcohol metabolizing enzyme activities due to thyroid hormones.

In order to achieve a hyperthyroid state, rats were treated for 7 days with thyroxine (150 microgram/100 g BW) or triiodothyronine (10 microgram/100 g BW). This regimen resulted in an enhanced activity of the microsomal ethanol oxidizing system. In addition, a decrease of hepatic alcohol dehydrogenase activity was observed under these experimental conditions, whereas hepatic catalase activity remained unchanged. These findings suggest that if chronic ethanol consumption simulates a functional "hyperthyroid hepatic state", increased rates of ethanol metabolism observed following prolonged alcohol intake might therefore be attributed at least in part to an induced activity of the microsomal ethanol oxidizing system in the liver.

Aldehyde Oxidoreductases↗
PubMed1980

Decreased hepatotoxicity of dimethylnitrosamine (DMN) following chronic alcohol consumption.

Compared to controls receiving physiological saline, the i.p. administration of dimethylnitrosamine (DMN) on 5 consecutive days to rats fed a nutritionally adequate liquid diet resulted 24 hours after the last injection of significant increases in glutamic dehydrogenase (GDH), glutamic oxylacetate transaminase (GOT), and glutamic pyruvate transaminase (GPT) activities in the serum, indicating a striking hepatotoxic effect of this compound. This was confirmed by the histological demonstration of massive centrolobular necrosis. Conversely, following pretreatment of the rats with an ethanol containing liquid diet for 23 days and subsequent administration of DMN the increases of serum enzyme activities and massive centrolobular necrosis could not be observed. These results therefore suggest that chronic alcohol consumption protects from hepatotoxicity due to DMN, most probably due to an enhancement of detoxifying pathways of the parent component or one of its toxic metabolites.

Alanine Transaminase↗
PubMed1980

Management of patent ductus arteriosus in preterm babies.

There is a higher incidence of delayed closure of the patent ductus arteriosus in premature babies with respiratory distress syndrome. From July, 1975, to December, 1977, 57 small, preterm infants with patent ductus arteriosus were diagnosed at our neonatal intensive care unit. From July, 1975, until September, 1976 (first period), 23 patients were diagnosed, and 11 underwent surgical ligation of a patent ductus arteriosus. There were 3 early deaths. From October, 1976, until December, 1977, out of a total of 34 patients with diagnosed patent ductus, 18 were treated with indomethacin, and only 3 required ligation. Our present policy for patent ductus arteriosus with respiratory distress syndrome in the premature baby is to initiate early treatment with indomethacin. If this treatment fails and the infant's status deteriorates, we perform early surgical ligation of the ductus in order to minimize the time on mechanical ventilation and lessen the chances of the development of bronchopulmonary dysplasia.

Ductus Arteriosus↗
PubMed1980

Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1.

tpo mutations, located at 74 min on the genetic map, rendered Escherichia coli K-12 resistant to TP1, a phage which can use either the OmpF protein or the LamB protein as its receptor. tpo mutants synthesized decreased amounts of OmpF and LamB proteins but increased amounts of the OmpC product, another outer membrane protein. The effect of the tpo mutations in lam B gene expression was transcriptional. It is one facet of the following effect on the maltose regulon: strong decreases in the syntheses of the LamB protein and the periplasmic MalE protein occurred when the regulon was uninduced; a lesser decrease occurred in the syntheses of the LamB protein the MalE protein, and the cytoplasmic MalQ protein (amylomaltase) when the regulon was induced. The tpo mutants were found to be phenotypically identical to the perA mutant recently described by Wanner et al. (J. Bacteriol. 140:229--239, 1979) and to some of the ompB mutants described by Verhoef et al. (Mol. Gen. Genet. 169:137--146, 1979). Mapping and complementation analysis suggested that these three types of mutations belong to the same cistron. Our results bring to at least four the number of clearly distinct phenotypes which can result from mutations at, or close to, ompB, a locus which appears increasingly complex.

Bacterial Proteins↗
PubMed1979

[Meconium aspiration syndrome with severe hypoxemia. Treatment with a pulmonary vasodilator (tolarzoline) (author's transl)].

Three patients with Meconium Aspiration Syndrome (S.A.M.) and severe hypoxemia are reported. From the first hours of life they needed mechanical ventilation, showing no improvement in PaO2 in spite of usual respiratory support measures. In one case, pulmonary hypertension and ductal right to left shunt through ductus arteriosus and foramen ovale was objetivated by cardiac catheterization. Following intravenous prefusion of a pulmonary vasodilator (Tolazoline) patients showed a clinical amelioration and a definite increase in PaO2. Results and evolution with the use of this drug are commented.

Ductus Arteriosus, Patent↗