Biomedical subjects

F Monaco

Publications and source records attributed to F Monaco.

At least 145 records · Page 8Linked to original sources

PubMed1975

Bioavailability of two carbamazepine preparations during chronic administration to epileptic patients.

Two preparations of carbamazepine, as tablets and in syrup, were given for 8 weeks to 20 patients with epilepsy in a randomized crossover study. Weekly plasma levels were 3-21 mg/1 on dosages of 5-24 mg/kg/day. There was no difference in absorption or steady-state level between the two preparations. Carbamazepine 10, 11-oxide was constantly present when the level of carbamazepine was over 5 mg/l, and tended to be lower with the syrup.

Administration, Oral↗

PubMed1975

Levels of free amino acids in serum and cerebrospinal fluid after administration of taurine to epileptic and normal subjects.

The modifications associated with taurine treatment of the free amino acid content of serum and cerebrospinal fluid were investigated in epileptic and control subjects. In patients with epilepsy the main findings were, in the serum, the correction toward normal of the amino acid levels that were low prior to therapy, and in the cerebrospinal fluid, the increase up to above-normal levels of glutamic acid, greatly diminished before treatment. Thus taurine, which has an anticonvulsant action appears to partially correct the amino acid imbalance in epileptics. The monitoring of taurine-induced glutamate changes in the cerebrospinal fluid could help to establish the correct therapeutic dose.

Adolescent↗

PubMed1975

Free amino acids in serum of patients with epilepsy: significant increase in taurine.

More than half the amino acids determined in serum were lower in patients with epilepsy than in control subjects. Taurine was the only amino acid to be increased in epilepsy. The changes could represent a compensatory metabolic reaction to limit the imbalance of amino acids in epileptic brain and to facilitate uptake of taurine, which has an anticonvulsant action.

Adolescent↗

PubMed1975

Soradic ulcerative mutilating acropathy with imbalance of free amino acids in the cerebrospinal fluid.

A case of sporadic ulcerative mutilating acropathy is described. Muscle biopsy showed signs of neurogenic atrophy. Motor nerve conduction velocity was slightly reduced. The muscles of the foot were completely unresponsive to electric stimulation of both femoral nerves. The content of free amino acids in serum and cerebrospinal fluid was determined by ion exchange chromatography, and compared with a control group. While in serum no significant changes were found, in CSF most amino acids showed great alterations, mainly consisting in an increase. It is suggested that the primary disturbance may be located in the spinal nerve roots.

Adult↗

PubMed1975

Defective thyroglobulin synthesis in an experimental rat thyroid tumor: iodination and thyroid hormone synthesis in isolated tumor thyroglobulin.

Purified rat tumor thyroglobulin from the experimental rat thyroid tumor, line 1-1C2, was studied for its thyroid hormone content after in vivo and in vitro iodination and compared with normal and desialylated normal rat thyroglobulin. Tumor thyroglobulin had a very low sialic acid and iodine content; after in vivo iodination it contained only small amounts of triiodothyronine (T3) and no detectable thyroxine (T4). After in vitro iodination with 125I it showed a distribution of T3 and T4 very similar to that of normal and desialylated normal thyroglobulin iodinated in vitro. In vitro iodination dissociated tumor and desialylated normal thyroglobulin to a greater extent than normal thyroglobulin. Tumor tissue, on the other hand, showed considerable iodinating activity in the 105,000 X g pellet when studied with exogenous acceptors. These results are compatible with a role for sialic acid in the maturation and migration of thyroglobulin to the iodination site, rpovided that the intracellular distribution of the iodinating enzymes are normal.

Animals↗

PubMed1975

Thyroglobulin biosynthesis in "cold" and "hot" nodules in the human thyroid gland.

Studies on "cold" and "hot" nodules from human thyroid gland have been carried out to investigate thyroglobulin biosynthesis and to correlate carbohydrates incorporation and thyroid hormone formation in thyroglobulin. The aim of the investigation was to ascertain the role of carbohydrates of the protein molecule in its migration to the iodinating site of the cell. Thyroid slices from two cases with "cold" and two with "hot" nodules were incubated for 30 to 120 min with [3H]leucine, [3H]carbohydrates (ManNAc, Gal, Man, GlcNAc, GalNAc) and 125I. Soluble and particulate iodoproteins, solubilized by digitonin, were identified by density gradient centrifugation and immunoprecipitation; following hydrolysis carbohydrates and iodothyronines were identified by paper chromatography and chemically determined. While the rate of leucine incorporation into TG increased with time in both "cold" and "hot" nodules, the "cold" nodule, with respect to the "hot" tissue, showed: much lower efficiency incorporating carbohydrates and iodine into TG; lower thyroid/medium ratio of radioiodine; less total soluble protein and soluble TG; more particulate protein and solubilized TG; 1/3 of the carbohydrate and iodine chemical content; only minute amounts of labeled iodothyronines in TG compared to normal levels in "hot" tissue. The formation of thyroid hormone, in enzymatically iodinated TG, and the iodinating activity of the 105,000 X g pellet, incubated with low iodine TG, were similar in both "cold" and "hot" tissues. These results demonstrate that the "cold" nodule is able to synthesize thyroglobulin but the protein is defective in its carbohydrate content. The deficient iodine transport into "cold" tissue and impaired hormonal synthesis are confirmed. The incomplete incorporation of carbohydrates into thyroglobulin in the "cold" nodule could be important for the maturation and migration of the molecule to the iodinating site of the cell

Acetylgalactosamine↗

PubMed1975