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Biomedical subjects

F Molina

Publications and source records attributed to F Molina.

123 records · Page 7Linked to original sources
PubMed1981

[Monoclonal IgG lambda paraprotein, Fanconi's syndrome of the adult, and osteomalacia (author's transl)].

The case here reported of 72 year-old female with osteomalacia in whom an adult's Fanconi's syndrome with distal tubular involvement and a monoclonal IgG-lambda paraprotein were discovered. There was urinary excretion of lambda light chains without evidence of myeloma or amyloidosis. Such apparently unrelated entities might be subject to an unitary pathophysiological approach: the nephrotoxicity of light chains could cause a Fanconi's syndrome, which in turn would give rise to osteomalacia through phosphate depletion. It is noteworthy that in this patient the urinary excretion of light chains was of the lambda type, in contrast to similar cases described in the literature which presented mostly kappa chains. The likelihood of this patient developing myeloma or amyloidosis at a later stage is discussed.

Aged↗
PubMed1977

Comparison between some digestive processes after eating and gastric loading in rats.

Physiological and biochemical differences were found between normal and intragastric feeding in the rat. Milk ingested orally remained in the stomach significantly longer than milk ingested via a chronic intragastric fistula. Milk samples were removed from the stomach at intervals of 5, 20 and 45 min after ingestion and it was found that milk which had been ingested via intragastric fistula underwent substantially less lipolysis than did milk which had been ingested orally. These results are discussed in relation to the aversive and rewarding effects obtained by injections of edible nutrients into the stomachs of rats.

Animals↗
PubMedDate not supplied

Nasal correction in Binder's syndrome: the evolution of a treatment plan.

Maxillofacial dysplasia, or Binder's Syndrome is a challenge for the surgeon. The evolution of a surgical treatment plan has led to improved facial contour and patient self-image. We studied 27 patients with maxillonasal dysplasia of variable degrees, both on a short- and long-term basis. In some patients, surgical treatment began as early as 3 years of age, while others were treated as teenagers or young adults. Surgical options included cartilaginous onlay grafts to the pyriform area, nasal dorsal grafts (linear or L-strut in design), and columellar strut grafts. Le Fort osteotomies were reserved for those patients with Class III malocclusion (15% in this series). The overall goals were to augment skeletal deficiencies of the midface and begin the soft tissue expansion process as early as possible. From our long-term follow-ups (up to 15 years) it has become apparent that surgical treatment should begin early. This leads to improved self-image by the child's preschool years, taking advantage from their youthful skin elasticity. In the young patient, sequential lengthening procedures of the dorsum and columella are beneficial. Paranasal and midfacial augmentation is reserved until midfacial growth is near complete when the patient is in their midteenage years.

Abnormalities, Multiple↗
PubMedDate not supplied

Chromosomal insertions localized around oriC affect the cell cycle in Escherichia coli.

The present work reports the effects of localized insertions around the origin of Escherichia coli chromosome, oriC, on cell cycle parameters. These insertions cause an increase of the C period with an inverse correlation to the distance from oriC. In addition, Omega insertion near oriC causes an increase in the number of replication forks per chromosome, n, and Tn10 insertion causes a decrease in growth rate. We found that the same insertion positioned in another region of the chromosome, outside of oriC, has a negligible effect on the C period. Marker frequency analysis suggests a slower replication velocity along the whole chromosome. We propose that the insertions positioned at less than 2 kbp from oriC could create a structural alteration in the origin of replication that would result in a longer C period. Flow cytometry reveals that asynchrony is not associated with these alterations.

Cell Cycle↗
PubMedDate not supplied

Different expressivity of two adjacent mutations of the APC gene.

AIMS AND BACKGROUND: The phenotypic expression of different APC mutations in familial adenomatous polyposis (FAP) is variable: two to three variants of the disease have been defined based on the severity of colonic manifestations. Age of onset and number of polypectomies per person-year of post-surgical follow-up were compared in two FAP families with very close mutation sites in the APC gene, in order to ascertain mutation-specific variation of expressivity. FAMILIES AND APC MUTATIONS: Family A (5 patients) carried a newly characterized mutation, a four bp deletion at codon 843. Family B (5 patients) carried a previously identified mutation at codon 835. RESULTS: Mean age of onset was 49.7 years in family A and 30.5 years in family B; number of polypectomies per person-year of follow-up was 1.05 for family A and 10.1 for family B (P < 0.001). CONCLUSIONS: There is significant variation of expressivity (allelic heterogeneity) in FAP between two mutations separated by only eight codons, located at the 5' extremity of APC gene exon 15.

Adenomatous Polyposis Coli↗
PubMedDate not supplied

[Primary neuroendocrine carcinoma of the pavilion of the ear].

We present a case of primary neuroendocrine carcinoma (Merkel cell carcinoma) of the external ear and its treatment. This paper also deals with histogenesis, pathology, clinical findings, diagnosis and treatment of this tumor.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

[Microbiological characteristics and spectrum of infections of 108 Streptococcus anginosus isolated].

BACKGROUND: The aim of the present study was to assess the key microbiologic characteristics aid in the identification of Streptococcus anginosus in the laboratory and know the spectrum of clinical infections it produces. METHODS: From March 1991 to April 1992 all the isolated strains of clinical significant samples were collected. For identification the API 20 STREP (BioMérieux) system was used. The histories of the patients with infection by S. anginosus were retrospectively reviewed to collect clinical and microbiologic data of interest. RESULTS: The 108 strains of St. anginosus isolated were diminute colonies on sheep blood agar with CO2 enrichment. They had a characteristic smell, were beta-hemolitic or non hemolitic and of the Lancefield group A, C, F, G or not groupable. All had invariable biochemical features in the API 20 STREP: produced acetone and alkaline phosphatase, hydrolized arginine, did not hydrolize hypurate nor did they ferment ribose. Streptococcus anginosus produced purulent infections of different localization: abdominal, head-neck, pleuro-pulmonary, muscle-skeletal, skin and soft tissues and miscellaneous. Most of these infections were polymicrobial (81.5%). CONCLUSIONS: St. anginosus was easily identified in the laboratory by its morphologic characteristics and biochemical reaction in the API 20 STREP. It produced a wide variety of supurative infections, some being severe: cerebral, pulmonary, hepatic, empyema abscess. The identification of this microorganism at a species level should contribute to better recognition as a pathogen of occassionally severe supurative infections.

Female↗
PubMedDate not supplied

[Antibiotic sensitivity of anaerobic bacteria isolated during one year (1993)].

BACKGROUND: The aim of this study was to determine the antimicrobian susceptibility of the anaerobe bacteria isolated from clinical samples in the authors' department in 1993. METHODS: The minimum inhibitory concentrations of 10 antimicrobians were determined in microgram/ml for 129 anaerobe bacterias by the microdilution in broth method recommended by the NCCLS. RESULTS: All The strains were sensitive to chloramphenicol. Metronidazole inhibited all the isolates except A. meyeri and 50% of other non spored gram-positive bacilli. Imipenen and piperacillin-tazobactam were the most active beta lactamics, with only 1 B. fragilis being resistant to both. Clavulanic amoxycillin presented good activity with only 5% from the B. fragilis group, 1 F. mortiferum and 2 P. anaerobius being resistant. In the B. fragilis group 10% were resistant to cefoxitin and 15% to piperacillin. The remaining isolates were sensitive to both antimicrobians. Resistance to clindamycin in the B. fragilis group was 16.7%. There were also strains resistant to Peptostreptococcus, Prevotella and aerotolerant non spored gram-positive bacilli. Penicillin and amoxycillin were the least active antimicrobians. CONCLUSIONS: Chloramphenicol, metronidazole, imipenem, piperacillin-tazobactam and clavulanic amoxycillin were very effective and showed a wide spectrum of activity versus the strains studied. Cefoxitin, piperacillin and clindamycin maintain good antianaerobe activity.

Anti-Bacterial Agents↗
PubMedDate not supplied

Improved performances of spot multiple peptide synthesis.

We have developed a new software for the design of peptides to be prepared by the manual Spot synthesis method. It covers most of the common protocols for epitope mapping and offers flexibility for planning the experiment. We have also quantified the coupling efficiencies of three different coupling methods for the synthesis of four model decapeptides. The DIC/HOBT procedure was superior (mean coupling yield per cycle: 87% to 91%) to methods using pentafluorophenyl esters of Fmoc amino acids. All four peptides prepared by the three different coupling protocols were reactive with their cognate monoclonal antibody. Finally, using the best coupling method, we synthesized peptides of increasing length (10 to 29 residues) bearing the model epitope AcPGK at the N terminus and showed that peptides of up to 29 residues were immunoreactive.

Antibodies, Monoclonal↗