Search PubMed⌕ Search

Biomedical subjects

F Milgrom

Publications and source records attributed to F Milgrom.

At least 109 records · Page 6Linked to original sources
PubMed1979

The use of staphylococcal protein A in the mixed agglutination test.

A mixed agglutination (MA) test employing staphylococcal protein A (SpA) instead of an antiglobulin reagent has been developed for the detection of IgG antibodies bound to cell-surface antigens. In the SpA MA test, a single indicator system may be used for the detection of IgG antibodies from several mammalian species. The sensitivity of this test compares favorably with that of the conventional MA test in the detection of most mammalian IgG. The main advantage of the conventional SpA MA test is its usefulness in the study of antigens on cells because of adherence of antibody-coated indicator erythrocytes.

Animals↗
PubMed1979

Mixed agglutination in microtiter U-trays.

The principle of mixed agglutination with cell cultures was applied to those cells which could not be obtained as monolayer cell cultures. Artificial monolayers of freshly isolated cells from various tissues were prepared in poly-L-lysine-coated wells of a microtiter U-tray. By means of this technique, murine alloantigens, H-2 private specificities and Thy-1 antigens were demonstrated on cells from thymus, brain, spleen, kidney and liver. Ia.2 antigen was detected on splenocytes but not on thymocytes or fibroblast L cells. Evidence was also presented that HLA A and B locus antigens could be demonstrated on human thymocytes. Sensitivity of this test was shown to be at least 100 times higher than that of the standard lymphocytotoxicity test for detection of HLA antigens.

Absorption↗
PubMed1979

Detection of a microsomal antigen and its antibody in human liver diseases.

Sera of 173 patients with various forms of liver disease along with serum precipitates produced by polyethylene glycol were screened for the presence of a microsomal antigen referred to as ubiquitous tissue antigen (UTA) and its antibody by double diffusion precipitation in agarose gel. UTA was detected in 7 or 26 patients with chronic active hepatitis, 1 of 5 with alcoholic hepatitis, 2 of 14 with alcoholic cirrhosis and 18 of 98 with hepatoma. Antibodies to UTA were found only in 2 patients with chronic active hepatitis, 1 with alcoholic cirrhosis and 1 with hepatoma. No UTA or its antibody were noted in sera of 5 patients with alcoholic fatty liver, 10 patients with hepatitis B, and 15 asymptomatic carriers of HBsAg. Positivity for the UTA or its antibody was restricted to severe, chronic cases irrespective of diagnosis, indicating that persistent tissue destruction might be necessary for antigen release or antibody formation.

Antibodies↗
PubMed1979

Paul-Bunnell antigen in malignancies and rheumatoid arthritis.

By means of agglutination inhibition test, Paul-Bunnell (P-B) antigen was demonstrated in sera of patients with cancer (5%), lymphomas or leukemias (12.5%) and rheumatoid arthritis (RA) (1.4%) as well as in synovial fluids from RA patients (27%). In contrast, none of 124 normal human sera gave positive results. P-B antigen was also demonstrated in 3 of 12 perchloric acid extracts of cancer tissues and 2 of 11 saline extracts obtained at 100 degrees C from spleens of lymphoma-leukemia patients. Extracts of apparently normal tissues from 16 individuals gave negative results.

Antigens, Neoplasm↗
PubMed1979

Hanganutziu-Deicher antigen and antibody in pathologic sera and tissues.

Heterophile, Hanganutziu-Deicher (H-D) antigen was studied in pathologic sera by means of inhibition of agglutination of bovine erythrocytes by H-D antibodies. H-D antigen was demonstrated in 38% of random cancer sera, 25% of lymphoma or leukemia sera, 25% of leprosy sera, 8% of infectious mononucleosis sera, 6% of rheumatoid arthritis sera, and 27% of synovial fluids of rheumatoid arthritis patients. None of 134 normal human sera gave positive results. Some of the inhibition-positive cancer sera formed precipitation lines with H-D antibody-containing sera. Over 50% of various extracts of cancer tissues as well as spleens of lymphoma or leukemia patients were shown to contain H-D antigen by means of the inhibition test.

Animals↗
PubMed1979

Dual response of lymphocytes from multiple sclerosis patients to myelin basic protein.

Peripheral blood lymphocytes from forty-nine patients with multiple sclerosis (MS), twelve patients with other neurological diseases and twenty-seven healthy individuals were examined by a two-stage procedure based on the Con A stimulation of the basic protein (BP) pre-treated lymphocytes. Enhanced BP-induced blastogenic stimulation (BS) (90--220% was observed in MS individual classified as active (A-MS), whereas suppressed BS (27--120%) was observed in MS patients classified as non-active (NA-MS). The difference was statistically significant (P less than 0.01). The results obtained with the MS patients' lymphocytes also differed significantly (P less than 0.05) from those of the two control groups. Either response obtained with MS lymphocytes could be elicited with a similar concentration of BP. The enhanced or suppressed response to BP did not correlate with the cellular response to common antigens such as candida and streptokinase-streptodornase. Most lymphocytes of individuals from the two control groups showed BS values of 100 +/- 20% in response to BP. However, some degree of stimulation or suppression was also obtained with lymphocytes from a few individuals of both control groups. The potential value and application of the two-stage procedure is discussed.

Adult↗