Search PubMed⌕ Search

Biomedical subjects

F Mendoza

Publications and source records attributed to F Mendoza.

At least 37 records · Page 2Linked to original sources

Detection of antibodies against avian antigens in bronchoalveolar lavage from patients with pigeon breeder's disease: usefulness of enzyme-linked immunosorbent assay and enzyme immunotransfer blotting.

The study reported here evaluated the usefulness of the enzyme-linked immunosorbent assay (ELISA) in the detection of antibodies against pigeon antigens in the serum and bronchoalveolar lavage (BAL) of patients with clinical, radiological, and functional evidence of interstitial lung disease (ILD) with and without pigeon breeder's disease (PBD). The results were compared with those obtained by the simultaneous use of counterimmunoelectrophoresis (CIE) in the same patients. In PBD, ELISA detected antibodies against pigeon's sera in both serum and BAL in 100% of patients, while CIE failed to detect the antibodies in the serum of one patient and in most of the samples of BAL. In addition, we used enzyme immunotransfer blotting to determine the number of epitopes in pigeon serum recognized by antibodies present in serum and BAL. There was a heterogeneous response in both fluids, but the reaction pattern demonstrated that patient's sera recognize to-25 different pigeon epitopes. We conclude that ELISA is a highly sensitive and specific method for the detection of antibodies against pigeon antigens in the serum and BAL of patients with PBD and that the host response involves a great number of avian antigens.

Alveolitis, Extrinsic Allergic↗

An evaluation of food group intakes by Mexican-American children.

Food group daily servings were examined for 3,436 children who participated in the Mexican-American portion of the 1982-1983 Hispanic Health and Nutrition Examination Survey (HHANES). Mean daily servings of 40 foods and food groups were calculated for four age groups: 1 to 2 years, 3 to 5 years, 6 to 11 years, and 12 to 17 years. The HHANES food servings data were combined into four major groups and compared with recommended servings for children. Mean daily servings of the milk group exceeded the recommended two to three servings for younger children but were low for teenagers. Meat group servings (including eggs and nuts/legumes) exceeded the recommended two daily servings for all age groups, whereas bread group intakes averaged 70% to 80% of a recommendation of four servings but only half of a recommendation of six servings. Intakes of fruits and vegetables were lowest, averaging only 33% to 47% of a recommended four servings, or 26% to 38% of a recommended five servings. Servings of all four groups were lowest for teenagers. A dietary score, based on the number of servings from each of the four groups, was developed for each child. Mean dietary scores ranged from 55% (teenagers) to 70% (toddlers and preschoolers) of the recommended score. According to these analyses, dietary guidance for Mexican-American children should focus on increasing intakes of fruits and vegetables and on encouraging more nutritious food choices by teenagers.

Adolescent↗

Effects of fructose feeding on blood parameters and blood pressure in impaired glucose-tolerant subjects.

The effects of fructose and glucose on selected blood parameters and blood pressure after 4 weeks of oral ingestion were investigated in nine impaired glucose-tolerant subjects (IGTS) and nine normal glucose-tolerant subjects (NGTS). The criteria for IGTS were (1) 1- or 2-hour postprandial glucose levels between 160 and 200 mg/dL (8.8 to 11.1 mmol/L); (2) compliance with the diet; and (3) no cardiac or renal complication. The mean increment in the area under the plasma glucose and insulin curves after a glucose load was significantly greater following glucose than following fructose ingestion in both IGTS and NGTS (p less than .05). Glycosylated hemoglobin was greater after glucose than after fructose ingestion in IGTS and NGTS (71 gm/L vs. 61 gm/L in IGTS; 61 gm/L vs. 59 gm/L in NGTS). Serum triglyceride level in IGTS fed glucose was 173 mg/dL (1.90 mmol/L), whereas in those fed fructose, it was 154 mg/dL (1.69 mmol/L). Serum cholesterol was also greater after glucose than after fructose ingestion in all subjects. However, the differences were not statistically significant. Urate, pyruvate, and lactate levels were significantly greater in IGTS fed glucose than in IGTS fed fructose. Systolic and diastolic pressures were lower in IGTS fed fructose than in IGTS fed glucose. The present study shows that a 4-week oral ingestion of moderate amounts of fructose appears to have more benefits than an equivalent glucose ingestion in both IGTS and NGTS. However, greater benefits were found in IGTS than in NGTS.

Blood Glucose↗

Antibodies to ribosomal ribonucleoprotein. Prevalence in systemic rheumatic diseases and partial characterization of the antigen.

The prevalence of antibodies to ribosomal ribonucleoproteins (rRNP) was studied in patients with rheumatic diseases. Seven patients had precipitating antibodies against rRNP, 6 had systemic lupus erythematosus, one had primary Sjögren's syndrome. Anti-rRNP was not present in mixed connective tissue disease, rheumatoid arthritis, progressive systemic sclerosis, CREST, primary Raynaud's or normal control sera. A partial immunological identity precipitin line was present between (U1) nRNP and rRNP, but these were distinct in physicochemical properties. Western blot analysis of ribosomal extract using anti-rRNP IgG revealed 2 polypeptides called rA and rB which appear to be the important antigenic determinants.

Antibodies↗

Entamoeba histolytica: specific antigen recognized by a monoclonal antibody.

Specific antigenic determinants on the membrane surface of Entamoeba histolytica that distinguish it from other Entamoeba species were demonstrated. Evidence for these antigenic determinants was obtained with a monoclonal antibody to E. histolytica which showed not only specificity but also sensitivity as demonstrated in enzyme linked immunosorbent assay. Immunofluorescence microscopy showed that the monoclonal antibody recognized an epitope present on the membrane surface of E. histolytica trophozoites. The epitope detected by the monoclonal antibody was present in three components of different molecular weight. These components may have a common precursor or may be the result of enzymatic degradation under the conditions tested.

Animals↗

Surgical procedures for the treatment of baldness.

New surgical methods are now available for the correction of male pattern baldness. Standard techniques have been significantly refined during the past decade. These methods include punch autografts, strip grafting, scalp reduction, use of hair-bearing flaps, microsurgery, and various combinations of these techniques. A more recent refinement of hair transplantation is the use of minigrafts and micrografts. We present an overview of the advantages and indications for each procedure.

Adult↗

Comparison of Bennett shift measured at the hinge axis and an arbitrary hinge axis position.

Bennett shift was measured at an arbitrary hinge axis position and at the hinge axis position in 10 subjects. Sixty percent of the arbitrary hinge axis positions were within 5 mm of the hinge axis position. There was no significant difference in the Bennett shift measurements made at the hinge axis and the arbitrary hinge axis positions when measured in 0.25 mm increments. The average immediate Bennett shift was found to be 1.05 mm on the left side and 1.12 mm on the right side when firm guidance was used.

Dental Articulators↗

Comparison of two methods for measurement of immediate Bennett shift.

IBS was measured by an intraoral interocclusal record and an extraoral method. The extraoral method gave significantly greater values statistically. Although more time consuming, the extraoral method provided a means of verification that is not obtainable by interocclusal records.

Dental Articulators↗

Effect of Ala-2-D-Trp-8-D-Cys-14-somatostatin on the arginine induced release of insulin, GH and glucagon in normal men.

The effects of several doses of somatostatin (SS) and the analog Ala-2-D-Trp-8-D-Cys-14-SS on the arginine-induced release of insulin, glucagon, and GH were compared in a group of normal human subjects. Somatostatin suppressed the release of insulin, glucagon and GH when administered in a dose of 100 micrograms/hr. The same dose of Ala-2-D-Trp-8-D-Cys-14-SS inhibited GH and glucagon release, but although insulin levels were decreased about 24%, this inhibition was not significant statistically. Higher doses of Ala-2-D-Trp-8-D-Cys-14-SS (300 micrograms/hr) induced a partial inhibition of insulin secretion and completely blocked GH release. These results indicate that in humans this analog is a less potent inhibitor of insulin release than somatostatin.

Adult↗

Pharmacologic management of Raynaud's phenomenon.

Raynaud's phenomenon (RP) represents a wide spectrum of disease activity. Specific pharmacologic therapy, when indicated, should be based upon the clinician's understanding of drug effectiveness and side effects. We present an update of currently available pharmacologic agents being used for RP and review the effectiveness and side effects of these drugs.

Blood Platelets↗