Biomedical subjects

F Marzatico

Publications and source records attributed to F Marzatico.

75 records · Page 5Linked to original sources

PubMedDate not supplied

Oxygen radicals and other toxic oxygen metabolites as key mediators of the central nervous system tissue injury.

Free radicals are species containing one or more unpaired electrons, and for this reason they are highly reactive and can combine with a great variety of biomolecules, changing their physico-chemical characteristics. Oxygen free radicals are normally produced during cellular metabolism and aerobic cells are provided with antioxidant defense mechanisms able to counteract this physiological production. In conditions of increased production or decreased scavenging of free radicals, they can assume a fundamental importance in the pathogenesis of acute or chronic brain diseases.

Aging↗

PubMedDate not supplied

Cellular and molecular events of ischemic brain damage.

Many events occurring during and after cerebral ischemia are well known, but they are not known enough to fully elucidate the mechanisms of brain damage. Energy failure alone cannot explain the functional damage occurring during the reperfusion phase, and, at present, four features of the ischemic and postischemic brain are the focus of interest: development of acidosis, calcium overload, free radical formation, and nitric oxide overproduction. It is likely that these events, perhaps with other less known ones, contribute altogether to the occurrence of irreversible damage. An understanding of them might lead to the development of a drug, or a drug cocktail, able to counteract ischemic lesions.

Acidosis↗

PubMedDate not supplied

Antioxidant enzymatic system and free radicals pathway in two different human cancer cell lines.

Resistance to oxidative damage is an important feature of cancer cells. Cellular anti-radical enzymes, lipid peroxidation, glutathione pathway, capability to produce ROS, and cells' susceptibility to H2O2 and menadione toxicity, were analyzed in DND-1A and HeLa cancer cell lines. SOD and GSHPx activities were higher in DND-1A than in HeLa cells. Lipid peroxidation was the same in both cell lines, while menadione stimulation of ROS production was tenfold higher in HeLa cells. Total and reduced, but not oxidized, glutathione levels, were tenfold smaller in HeLa cells. H2O2 proved fatal to HeLa cells after 12 hours' incubation, while it was ineffective on DND-1A; DND-1A cells were more sensitive to menadione toxicity than HeLa cells. The two lines behaved differently in response to the above treatments. These observations might be important in designing more specific cancer treatments.

Antioxidants↗