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Biomedical subjects

F Manzarbeitia

Publications and source records attributed to F Manzarbeitia.

29 records · Page 2Linked to original sources
PubMed1996

Role of endothelin in the pathophysiology of renal ischemia-reperfusion in normal rabbits.

The present study addressed the acute effects of endothelin-1 on renal function and neutrophils accumulation in the setting of in vivo severe (60 min) acute ischemia/reperfusion. Ischemia/reperfusion decreased renal functional parameters and increased renal neutrophil accumulation and medullary congestion. All these parameters markedly improved with the intrarenal administration of anti-endothelin-1 antiserum. Comparatively, the intrarenal infusion of endothelin-1 decreased renal function and increased neutrophil accumulation. Abnormalities in renal histology were, however, less pronounced than with ischemia/ reperfusion. In experiments using rabbit isolated perfused kidneys, endothelin-1 induced the accumulation of labeled neutrophils. This accumulation was similar to that observed in kidneys obtained after 60 minutes of ischemia plus 60 minutes of reperfusion. Both endothelin and ischemia/ reperfusion effects were counteracted by an anti-endothelin antibody. In further in vitro studies, we found that endothelin-1-induced the expression of the CD18 antigens on the neutrophil surface. In subsequent experiments based on this effect of ET-1 on CD18 antigens, a blockade of both ischemia/reperfusion-induced and endothelin-1-induced neutrophil accumulation was obtained by infusion an anti-CD18 antibody. In conclusion, our experiments disclosed the critical role of endothelin-1 as a major promoter of early neutrophil accumulation after ischemia/reperfusion, which occurred through an integrin-mediated mechanism.

Animals↗
PubMed1996

Role of endothelium-related mechanisms in the pathophysiology of renal ischemia/reperfusion in normal rabbits.

The present study addressed the effect of interventions aimed to increase NO in the setting of acute renal ischemia/reperfusion (I/R) in uninephrectomized rabbits. In the 60-minute post-I/R period, L-arginine+superoxide (O2.-) dismutase (SOD) synergistically improved the renal functional (69.4% versus 10.4% of the pre-I/R glomerular filtration rate with or without L-arginine+SOD, respectively; p < .01) and histological parameters (82.9% decrease of medullary congestion in L-arginine+SOD, P < .01 versus vehicle) and blocked the I/R-dependent neutrophil accumulation (89.3% reduction). In spite of these results over the short term, a second set of experiments disclosed that the protection by L-arginine+SOD was no longer present at 24 and 48 hours (plasma creatinine in vehicle-treated versus L-arginine+SOD-treated animals [mg/100 mL]: 24 hours after I/R, 9.4 +/- 1.9 versus 8.07 +/- 0.65; 48 hours after I/R, 11.6 +/- 3.6 versus 9.7 +/- 0.9; P = NS in all the cases). Additional experiments were conducted using a milder 30-minute ischemic model, which showed no significant functional or histological protection by using L-arginine+SOD. In conclusion, our experiments disclosed the following: (1) the critical importance of the interaction between NO and O2.- in the acute protective effect of L-arginine (this effect not only improved renal function and histology but also reduced neutrophil accumulation) and (2) the discordance existing between the immediate protection afforded by L-arginine+SOD and the lack of protection observed at 24 and 48 hours. This finding suggests that a punctual intervention on the NO system at the time of I/R is not sufficient to reduce renal damage over the long term.

Animals↗
PubMed1995

[Skin fragments carried by spinal needles in cadavers].

Epidural or intradural puncture with inappropriately stiffened or improperly placed needles can carry cells or fragments of epithelial tissue into the epidural or intradural space. These skin fragments feed by imbibition, possibly leading to the development of epidermoid cysts. We aimed to study the ability of today's needles to transport cells or epithelial fragments. We studied 120 needles in 6 groups of 20, as follows: group 1, Touhy G-16; group 2, Touhy G-17; group 3, Quincke G-22; group 4, Quincke G-26; group 5, Sprotte G-22, and group 6, Sprotte G-24. These needles were used to make intradural and epidural insertions, as indicated, with stiffeners fully in place. Insertions were made into 3 cadavers, epidermal cells or skin fragments were then isolated from the solutions used to wash the needles, and the samples were studied under an optical microscope. We identified groups of cells or epidermal tissues in 45% of the Touhy G-16 samples and in 30% of the Touhy G-17 samples. Squamous epithelial cells were found in 15% of the Quincke G-22 samples and in 30% of the Sprotte G-22 samples. There was a significant difference between the amount of tissue transported by the Touhy needles in comparison with the Quincke (p < 0.01) and Sprotte (p < 0.05) needles. Needles from some manufacturers transport epithelial fragments during lumbar puncture. We believe that better quality control during manufacture of epidural and intradural needles can help to eradicate the rare neurological complications derived from the removal of epithelial cells and their subsequent deposit inside the spinal channel.

Cadaver↗
PubMed1992

[Renal cholesteatoma: keratin accumulation tumor].

Presentation of 6 cases of renal cholesteatoma in 4 male and 2 female patients ranging between 30 and 67 years of age. The most consistent clinical data was a history of relapsing nephritic colic of long-evolution. The average time to diagnose was 19 years. In 50% cases an association to malignant neoplastic pathology was found. The clinical diagnosis was based on the urography and the histopathological examination of the material passed with the urine. Renal exeresis was performed in 5 cases. One was treated in a conservative fashion. Also the etiology causes, diagnostic procedure and other therapeutic possibilities were reviewed.

Adult↗
PubMed1992

[Anisakiasis in Spain. Report of 3 cases].

We reported here three cases of non-imported anisakiasis in Spanish patients. Eating raw sardines was the only common epidemiological feature. Two of the cases presented clinically as a bowel obstruction, and underwent an emergency surgical procedure. In the intestinal biopsy a larva of Anisakiasis simplex was found in the abdominal wall, surrounded by a eosinophilic granuloma and large edema. The third case presented as a peptic ulcer disease with longer evolution time, and a larva of Pseudoterranova decipiens (Phocanema) was found during an upper gastrointestinal endoscopic procedure.

Adult↗