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Biomedical subjects

F Lozano

Publications and source records attributed to F Lozano.

At least 109 records · Page 6Linked to original sources
PubMed1989

Heterogeneity in the electrophoretic mobility of CD5 molecules after phorbol ester stimulation.

As judged by Western blot analysis, phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate (PDBU) induce the rapid and dose-dependent appearance of slower mobility CD5 molecular forms from peripheral blood mononuclear cell (PBMC) and thymus cell lysates. This phenomenon was inhibited by staurosporine, suggesting that it can be mediated by PKC activation. Furthermore, under our experimental conditions, neither Concanavalin A, nor Phytohaemagglutinin P or the calcium ionophore A23187 were able to reproduce the phorbol ester-induced changes in the CD5 electrophoretic mobility. When immunoprecipitated from phorbol ester-stimulated P32 labelled PBMC lysates, the slower mobility of CD5 molecules was associated to important phosphorylation. This special electrophoretic behaviour after phorbol ester-stimulation makes CD5 different from other lymphocyte surface glycoproteins and may have important implications in the elucidation of the biological role of this molecule as discussed below.

Alkaloids↗
PubMed1989

Leptospira abortion in horses.

Leptospira infection was diagnosed as the cause of 4 late-term equine abortions/stillbirths and 1 neonatal death in Louisiana. The most consistent gross and microscopic lesions were icterus and interstitial nephritis, respectively. Diagnoses were based on visualization of compatible spirochetes in Warthin-Starry-stained sections of kidney, liver, and placenta. Confirmation by immunofluorescence was made in 2 cases.

Abortion, Veterinary↗
PubMed1988

Subacute cutaneous lupus erythematosus: clinicopathologic findings in thirteen cases.

The clinicopathologic and serologic findings of thirteen patients with subacute cutaneous lupus erythematosus are reported. The clinical and immunologic features are similar to those described by most other authors. From a histologic point of view, however, two aspects could be emphasized: the presence of a larger number of epidermal colloid bodies and severe epidermal necrosis (more than 60% of cases). All patients with this microscopic picture have a similar clinical and serologic pattern: annular lesions, anti-Ro antibodies and human leukocyte antigen-DR3.

Adult↗
PubMed1988

An unusual cause of lymphoedema--confirmed by isotopic lymphangiography.

We describe here a rare case of lymphoedema (Anderson-Fabry's disease) and its examination using isotopic lymphography. Anderson-Fabry's disease gives rise to various multisystem problems including lymphoedema which are all secondary to the deposition of glycosphingolipids in venous tissue. The prognosis particularly in homozygote males is poor.

Adult↗
PubMed1987

Transient immunologic defect in a case of Listeria rhombencephalitis.

We report a case of Listeria rhombencephalitis in a previously healthy 60-year-old man. Listeria rhombencephalitis is a rare but well-defined clinical syndrome of lower brain-stem involvement caused by Listeria monocytogenes. Contrary to other listerioses, rhombencephalitis has been mainly observed in patients without predisposing conditions. In our case, however, findings of a detailed immunologic study, performed three months and one year, respectively, after clinical onset of Listeria rhombencephalitis manifestations, showed a transient cellular immunity defect, not associated with any other apparent disease.

Encephalitis↗
PubMed1987

CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes.

In this study data are presented indicating that the molecule identified by the recently described CD43 cluster of monoclonal antibodies (mAb; Oxford, 1986) is the human analogue to the one originally described in rat as leukocyte sialoglycoprotein (LSGP). This conclusion is based on several criteria. Both molecular mass (105 kDa) and cellular distribution are similar to the antigens previously described in the rat with the mAb W3/13 and subsequently in humans with L10 mAb. Sialidase treatment of the molecule immunoprecipitated by 84-3C1 mAb (CD43) resulted in a decreased electrophoretic mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. L10 mAb inhibited the binding of 84-3C1 mAb to the cell membrane suggesting that both mAb recognized the same molecule. Moreover, the presence in the CD3-4-8- thymic populations of the sialidase-sensitive epitope recognized by mAb 84-3C1 suggests that there is no simple correlation between the thymic maturation and the degree of sialylation.

Amino Acids↗
PubMed1985

Laboratory control of continuous intravenous heparin therapy with Howel time and activated partial thromboplastin time. A prospective, randomized and blind study.

Although heparin has been used extensively to treat Deep Venous Thrombosis (DVT) and arterial ischemia (AI), controversy still exists regarding optimal dosage and the need for monitoring. Different authors have employed various test with variable results. Others, however, persist in giving heparin without laboratory control. This study was made in order to compare, in a prospective, randomized and blind manner, two coagulation tests, namely: Howel Time (HT) and Activated Partial Thromboplastin Time (APTT), in controlling the dose of heparin given by continuous intravenous infusion in DVT and AI. Our results show no significant difference in complications and failures of the therapy with either test, although significantly higher doses of heparin were needed to maintain APTT within therapeutic range than those needed to keep HT within a similar range.

Aged↗
PubMed1984

Concentrations of cefmetazole in plasma and tissue resulting from peri-incisional administration before appendectomy.

The levels of cefmetazole in plasma and tissue were determined after injection of a dose of 30 mg/kg into the zone of the wound of each of 15 patients undergoing appendectomy. The mean plasma levels of cefmetazole at the start and end of surgery (8.9 +/- 2.4 and 31.7 +/- 6.4 min after dosage) were 21.1 and 59.0 micrograms/ml, respectively; concentrations of the drug were 14.6 and 4,486.9 micrograms/g in skin, 9,165.0 and 1,756.4 micrograms/g in subcutaneous tissue, and 8,669.6 and 2,022.9 micrograms/g in muscle.

Administration, Topical↗