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Biomedical subjects

F Liu

Publications and source records attributed to F Liu.

At least 361 records · Page 20Linked to original sources
PubMed1999

[Nutritional impact of glycyl-glutamine dipeptide on the autotransplanted small intestine in pig].

OBJECTIVE: To observe the impact of glycyl-glutamine (Gly-Gln) dipeptide on the autotransplanted small bowel. METHODS: Ten unbred pigs underwent small bowel autotransplantation and were divided randomly into two groups: GTPN and STPN. In the GTPN group (n = 5), the pigs received TPN supplemented with Gly-Gln for 28 days, whereas in the STPN group (n = 5), the pigs received isonitrogenous and isocaloric standard TPN for 28 days. The content of plasma glutamine (Gln), the concentrations of mucosal Gln, protein, villous DNA and RNA, villous height, mucosal thickness, crypt depth, villous surface area, D-xylose absorption test, and intestinal permeability to (99m)Tc-DTPA were measured on the operative day or on the 1st and 28th day after operation. RESULTS: All the indexes except the intestinal permeability (IP) to (99m)Tc-DTPA were significantly higher in the GTPN group than in the STPN group (P < 0.05) on the 28th day after operation, but the IP to (99m)Tc-DTPA was significantly lower in the GTPN group than in the STPN group (P < 0.05). CONCLUSIONS: Gly-Gln has special nutritional effect in improving the metabolism, structure and function of autotransplanted small intestine in pig.

Animals↗
PubMed1999

[Relationship between insulin-like growth factor II and prognosis of colorectal cancer].

OBJECTIVE: To investigate the relationship between insulin-like growth factor II (IGF-II) and prognosis of colorectal cancer. METHODS: One hundred and forty-two colorectal cancer patients were enrolled. In colonoscopic biopsy specimens, the expression of IGF-II and PCNA were detected immunohistochemically, while TUNEL technique was used to detect apoptosis. All patients were followed up, and disease-free survival (DFS) and overall survival (OS) rate were calculated. RESULTS: The expression level of IGF-II was significantly higher in colorectal cancer than in normal colorectal mucosa. A correlation was observed between more IGF-II expression, high PCNA labeling index, and apoptotic index was demonstrated. Patients with lower expression level of IGF-II had higher DFS and OS. Multivariate analysis by means of the Cox proportional-hazards model revealed that the expression level of IGF-II was an independent prognostic predictor in colorectal cancer patients. CONCLUSION: The expression level of IGF-II is a new prognostic predictor for colorectal cancer.

Adenocarcinoma↗
PubMed1999

[Evaluation of potential harmful effects of organic pollutants in tap water on mice in vivo].

OBJECTIVE: To study the harmful effects of organic concentrates from tap water on mammal animals in vivo. METHODS: Organic pollutants were absorbed with resin XAD-2 from tap water and administered the mice via a stomach tube or intraperitoneal injection. Micronucleus rate in bone marrow polychromatic erythrocytes, testicular chromosome aberration rate, indices for immune functions and the activity of ethoxyresourufin o-deethylase (EROD) in S(9) fraction of the liver were determined. RESULTS: Micronucleus rate and chromosome aberration rate increased by 1.7 and 3.5 folds, respectively, after administration of high dose of organic pollutants from the tap water (200 liters of tap water per kilograms of body weight) for three days. Humoral immunity and non-specific immune functions were obviously inhibited after administration of low-dose (6 L/kg). In addition, organic concentrates from tap water also could significantly induce the activity of EROD, with a good dose-dependent relationship. CONCLUSION: Organic concentrates from tap water could cause chromosome damage to somatic and germ cells and decrease in immune functions in the mice, as well as they could induce the activity of cytochrome P448 in the liver microsome. It suggested that organic pollutants concentrated from tap water had potential carcinogenic effects on mammal animals.

Animals↗
PubMed1999

[The detection of microsatellite instability in carcinoma and adenoma of large bowel and its significance].

OBJECTIVE: To explore the role of microsatellite instability (MSI) in colorectal carcinogenesis and the relationship between MSI, proliferation activity and prognosis. METHODS: PCR-SSLP and immunohistochemistry methods were used to detect MSI and the expression of PCNA in 56 cases of carcinomas, 9 cases of adenomas and 6 cases of adenomas with malignant changes. RESULTS: The total positive rate of MSI were 25/56 cases in colorectal carcinomas. The MSI positive cases were 3/4 in HNPCCs, 22/52 in sporadic colorectal carcinomas, 2/9 in adenomas and 2/6 in adenoma with malignant changes respectively. The PCNA labelling index of MSI positive tumors were significantly lower than that of MSI negative tumors (P < 0.01). The 3 and 5 year survival rates in patients with MSI positive colorectal carcinomas were higher than those of MSI negative tumors. CONCLUSION: MSI may be an early molecular alteration and another molecular mechanism in colorectal carcinogenesis. The MSI positive tumors have low proliferating activity and a better clinical outcome. The detection of MSI might be useful in predicting prognosis of colorectal carcinomas.

Adenoma↗
PubMed1999

[Effects of danshen and shengmaiye on glomerulosclerosis by adriamycin in rats].

OBJECTIVE: To investigate the effects of danshen(Salvia plectranthoides Griff.) and shengmaiye (Panax ginseng C. A. Mey, Ophiopogon japonicus Ker-Gawl and Schisandra chinensis Baill) on glomerulosclerosis induced by adriamycin in SD rats. METHODS: Left kidney of the animals was removed and after 7 days, adriamycin(6 mg.kg-1) was injected through tail vein, so as to establish an animal disease model. Then danshen or shengmaiye was injected to peritoneal cavity of the rats. All rats were killed by the end of the 8th week. Hemoglobin, BUN, cholesterol and protein in urea within 24 hours(ur Pro/24 h) were detected. IV collagen(IV col.) and laminin(LN) of renal cortex were determined by ELISA. The quantitation of IV col and LN in glomerular mesangial area was analyzed by computer pictures. RESULTS: Compared with model-danshen group(Group III), model-shenmaiye group(Group IV) and normal control group(Group I), BUN, cholesterol(Ch) and ur Pro/24 h were increased obviously and hemoglobin(Hb) was decreased in model control group(Group II). The values of BUN, Ch and Ur Pro were lower in Group III and Group IV than those in Group II and higher than those in Group I (P < 0.05). The quantitation of IV col and LN within renal cortex and mesangial area was less in Group III and Group IV than that in Group II and more than that in Group I (P < 0.05 or P < 0.01). CONCLUSION: Dansheng and shengmaiye may play an important role in the treatment of glomerulosclerosis in rats.

Animals↗
PubMed1999

[Determination on chemical constituents of Curcuma L. produced in China].

OBJECTIVE: To provide some valuable information for the research on species systematization and quality standardization of Curcuma drugs. METHODS: Colorimetry, TLC-colorimetry and water vapour distillation were used to determine the contents of curcuminoids, curcumin and essential oils of 34 samples of Curcuma produced in China. RESULTS AND CONCLUSION: The contents of curcuminoids and curcumin are different upto an extent of over 100 times among the varieties of Curcuma, and those of essential oils to an extent of 6 times. But there is little difference in the samples of the same species from different producing areas. There is also an obvious correlativity between the contents of curcuminoids and essential oils in the varieties of curcuma.

Curcuma↗
PubMed1999

[Influence of decocting time on the amount of anthraguinone diffused from Cassia seeds].

OBJECTIVE: To study the influence of decocting time on the amount of anthraquinone diffused from the cassia seeds. METHOD: The amount of anthraquinone in the decoctions made in different lengths of decocting time was determined by spectrophotometry (for intact seeds and powdered seeds). RESULT: Fast nonlinear diffusion district, slow linear diffusion district and ceasing diffusion district were shown on the light absorption-decocting time curves, and appropriate smashing of the cassia seed facilitated the diffusion of anthraquinone. CONCLUSION: The best decocting time for anthraquinone to diffuse from the cassia seeds should be the ending time of the fast nonlinear diffusion district, while the second decoction should not be given up.

Anthraquinones↗
PubMed1999

[The influence of nasal blood flow after ligating external carotid artery and its branches in rabbits].

OBJECTIVE: To study the influence of nasal blood flow after ligation of external carotid artery and its branches. METHOD: We ligate different levels of external carotid artery and its branches, and use laser Doppler flowmetry to observe the change of blood flow in nose before and after operation. RESULT: Among the four types of operation ligating different blood vessels to reduce the blood flow of the operating side, the most effective way is to ligate ipsilateral carotid artery and its branches in the same side. CONCLUSION: After ligation of relating blood vessels, the collateral circulation construct very soon, and the founding speed of that is quicker in the early stage.

Animals↗
PubMed1999

[Clinical study of treatment of epistaxis of little's area by microwave, anterior nasal packing and trichloroacetic acid cautery].

OBJECTIVE: To study the difference and quality of three different kind of treatment methods for epistaxis of Little's area, such as: microwave, anterior packing of nasal cavity and trichloroacetic acid cautery. METHOD: The cases were divided into three group. The state of bleeding, reaction of after-treatment and recurrence were recorded and analysed. RESULT: Comparing with the anterior nasal packing and trichloroacetic acid cautery, the microwave is characteristic by thorough stop-bleeding, light reaction, less recurrence. CONCLUSION: Microwave is an ideal way to treat epistaxis of Little's area. The patient's reaction is mild and less the clinical effects is obviously better than the anterior packing of nasal cavity and trichloroacetic acid cautery.

Adolescent↗
PubMed1999

[Immunohistochemical study of the expression of fucosylated glycoconjugates in human lung carcinoma].

Lewis X, Sialyl Lewis X and Sialyl Dimeric Lewis X are three fucosylated glycoconjugates on cell surface. With immunohistochemical method, the expression of the three structures in the original lung cancer tissues (with or without metastasis), adjacent tissues and metastatic lesions of lung carcinoma were studied. It was found that the three antigens were expressed with different intensity on the cell surface and in the cytoplasm of the lung carcinoma cell. However, there was no or only trace expression of these antigens in the adjacent tissues of lung carcinoma and normal lung tissues. Moreover, the original lesions of lung carcinoma with metastasis and/or poor differentiation expressed higher level of the three antigens than those without metastasis and/or with well or medium differentiation. Sialyl Lewis X was considered to be more closely related to the metastatic ability and differentiation of the lung carcinoma cell than the other two antigens, Lewis X and Sialyl Dimeric Lewis X. Furthermore, in the lymph nodes with lung carcinoma cell metastasis, there were expression of the three antigens with different degree, while in those lymph nodes without lung carcinoma cell metastasis, there was no expression of the three antigens.

Glycoconjugates↗
PubMed1999

[Quantitative analysis of butachlor, oxadiazon and simetryn by gas chromatography].

The quantitative analysis of the ingredients in 26% B-O-S (butachlor, oxadiazon and simetryn) emulsion by gas chromatographic method was carried out with a 5% SE-30 on Chromosorb AW DMCS, 2 m x 3 mm i.d., glass column at column temperature of 210 degrees C and detector temperature of 230 degrees C. The internal standard is di-n-butyl sebacate. The retentions of simetryn, internal standard, butachlor and oxadiazon were 6.5, 8.3, 9.9 and 11.9 min respectively. This method has a recovery of 98.62%-100.77% and the coefficients of variation of this analysis of butachlor, oxadiazon and simetryn were 0.46%, 0.32% and 0.57% respectively. All coefficients of linear correlation were higher than 0.999.

English Abstract↗
PubMed1999

[The basic study of noninvasive detecting the ischemic/hypoxic cerebral injury].

The cerebrovacular diseases are important causes of mortality and disability in the world, and with the development of science and technology, many mission-oriented basic researches are aimed at the ischemic/hypoxic brain injury. This paper introduces some progress made in our research in this field, which includes: the animal experiment model of brain injury; the technique to efficiently extract evoked potentials from electroencephalogram; and the identification of the characteristic information of brain injury.

Animals↗
PubMed1999

[Texture analysis of B-scan image using fractal dimension].

Based on the texture analysis of B-Scan image fractal model, this paper presents the liver clinical B-Scan images and their fractal characteristics. It describes the semi-fractal characteristics of B-Scan images and calculates the fractal dimension(D) of the liver B-scan images of 10 normal persons, 3 patients with cancer of the liver, and 3 patients with cirrhosis of the liver. The results indicate that Fractal Dimension (D) is an important parameter in differentiating some diseases. On the basis of the influence of Fractal Dimension (D) and scale (epsilon), this paper suggests the texture classified parameter S. The experiment results indicate that the parameter S can be used to differentiate diseases efficiently.

Algorithms↗
PubMed1999

[New parameter of liver ulitasonic tissue characterization--multi-fractal spectrum].

Based on the previous research about the feature of liver ultrasonic images, this paper presents a new analysis method of multifractal spectrum alpha-f(alpha). Using a series of partition Hausdorff dimension f(q) this paper describes the sophisticated fractal characteristics of the ultrasonic images in the different section of the growing process and the multi-fractal spectrum alpha-f(alpha) acts as the new parameter of the ultrasonic issue characterization. Using the neural network it has catalogued and recognized the tissue diseases, caring out ultrasonic tissue characterization. Apparently, this method has practical significance.

Algorithms↗
PubMed1998

Sequence dependence of branch migratory minima.

The Holliday junction is a central intermediate in the process of genetic recombination. The position of its branch-point can relocate through an isomerization known as branch migration. This migration occurs because the branch-point is flanked by homologous symmetry. All attempts at modeling the kinetics of branch migration have relied on the assumption that branch migration minima are sequence-independent. We have tested that assumption here, using a competition assay based on symmetric immobile branched junctions; these are junctions that cannot undergo branch migration, despite the fact that they are flanked by homology. The assay used is predicated on the non-association of strands displaced in the assay; we have tested this assumption, and have performed our experiments under conditions where we know that it is true. We have measured the free energy of relocating a branched junction from a fixed non-homologous sequence to all possible dimeric symmetric sequences. We find that the assumption of sequence-independence is often valid, but that it is not universally true. We find that the flanking sequences can have a marked effect on the free energy measured, both for extensions of symmetry and for reversals of flanking nucleotides. We have varied the temperature in our experiments, and have derived both enthalpies and entropies for the different sequences. The entropies are largely unfavorable, whereas the enthalpies are largely favorable; regardless of the signs of these quantities, we see that this is another system where enthalpy-entropy compensation is operative.

Base Sequence↗