In a previous study, weight gain, insulin growth factor-1 (IGF-1) and insulin growth factor binding protein-3 (IGFBP-3) were increased in rats fed suboptimal levels of energy and administered 0.1 mg/100 g of body weight of recombinant human growth hormone (rhGH). Our objective was to determine whether these anabolic effects were still obtained with lower dosages of rhGH in similarly fed rats. Three groups of male, prepubertal Sprague-Dawley rats were administered rhGH and three groups of similar rats were given normal saline solution daily (0.05 mg/100 g of body weight subcutaneously). All rats were fed a balanced 1:1 carbohydrate:fat ratio diet for 4 wk. Restricted rats within each treatment were pair fed 80% and 60% ad libitum. Daily body weight, food intake, and efficiency were recorded. After 4 wk, serum IGF-1 and IGFBP-3, body fat, fat-free mass, and total body water were determined. Total weight gain and serum IGFBP-3 were significantly higher, with a tendency for increased body fat, in rats treated with rhGH and fed at 60% ad libitum. However, within each treatment, energy restriction caused decreased body fat and total body water. These results suggest that lower dosages of rhGH provide anabolic effects during suboptimal energy intake.

Animals↗

PubMedDate not supplied

Reported dietary intakes of patients with insulin-dependent diabetes mellitus: limitations of dietary recall.

Reported dietary intakes were assessed in young patients with insulin-dependent diabetes mellitus (IDDM). We studied 44 IDDM patients (24 males, 20 females, mean +/- SD age 13.2 +/- 4.5 yr) and compared them with 44 healthy age- and sex-matched control subjects. Estimated intakes from 24-h dietary recall were analyzed in relation to body weight and degree of diabetes control. The reported energy intake of the IDDM patients with greater than 120% ideal body weight (IBW) for height was 66, 59/88% (where X = geometric mean, L1 = lower confidence limit/L2 = upper confidence limit) of recommended daily allowance (RDA), whereas those with IBW less than 120% reported 90, 67/120% (p less than 0.01). Patients with increased weights in comparison with IBW had higher hemoglobin A1c (HbA1c) levels (11.9 +/- 2.7%) than those with weights more appropriate for IBW (9.7 +/- 2.4%, p less than 0.025). IDDM patients reported overconsumption of protein and fat, but their carbohydrate intake was low. Analysis of dietary recalls revealed high protein intake (X +/- SD, 20.0 +/- 5.0% of total calorie intake), especially in older (27 +/- 4%) compared with younger (19 +/- 2%-19 +/- 4%, p less than 0.01) patients. Proportions of carbohydrate, protein, and fat did not correlate with variations in body weight and/or HbA1c. The reported intake of protein per kilogram body weight was not significantly different between appropriate-weight and overweight IDDM patients. There was no significant difference in reported total energy intakes of IDDM patients compared with their healthy control subjects.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗