Biomedical subjects
F Lemeunier
Publications and source records attributed to F Lemeunier.
The evolutionary genetics of the hobo transposable element in the Drosophila melanogaster complex.
Hobo elements are a family of transposable elements found in Drosophila melanogaster and its three sibling species: D. simulans, D. mauritiana and D. sechellia. Studies in D. melanogaster have shown that hobo may be mobilized, and that the genetic effects of such mobilizations included the general features of hybrid dysgenesis: mutations, chromosomal rearrangements and gonadal dysgenis in F1 individuals. At the evolutionary level some hobo-hybridizing sequences have also been found in the other members of the melanogaster subgroup and in many members of the related montium subgroup. Surveys of older collected strains of D. melanogaster suggest that complete hobo elements were absent prior to 50 years ago and that they have recently been introduced into this species by horizontal transfer. In this paper we review our findings and those of others, in order to precisely describe the geographical distribution and the evolutionary history of hobo in the D. melanogaster complex. Studies of the DNA sequences reveal a different level of divergence between the group D. melanogaster, D. simulans and D. mauritiana and the fourth species D. sechellia. The hypothesis of multiple transfers in the recent past into the D. melanogaster complex from a common outside source is discussed.
Invasion of the hobo transposable element studied by in situ hybridization on polytene chromosomes of Drosophila melanogaster.
The invasion kinetics of hobo transposable element in the Drosophila melanogaster genome was studied by in situ hybridization on the polytene chromosomes. Six independent lines of Drosophila melanogaster flies that had been previously transformed by microinjection of the pHFL1 plasmid containing a complete hobo element were followed over 50 generations. We observed that hobo elements were scattered on each of the chromosome arms, with more insertion sites on the 3R arm. The total number of insertion sites remains quite small, between four and six, at generation 52. On the 2R arm, a short inversion appeared once at generation 52. Most of the integration sites reported here were already described for several transposons but some of them appear to be hotspots for hobo elements.
Population dynamics of the copia, mdg1, mdg3, gypsy, and P transposable elements in a natural population of Drosophila melanogaster.
The insertion site polymorphism of the copia, mdg1, mdg3, gypsy, and P transposable elements was analysed by in situ hybridization to the polytene chromosomes in genomes of males from a natural population of Drosophila melanogaster. Parameters of various theoretical models of the population biology of transposable elements were estimated from our data, and different hypotheses explaining TE copy number containment were tested. The copia, mdg1 and gypsy elements show evidence for a deficiency of insertions on the X chromosomes, a result consistent with selection against the mutational effects of insertions. On the contrary, mdg3 and P copy numbers fit a neutral model with a balance between regulated transposition and excisions. There is no strong evidence of a systematic accumulation of elements in the distal and proximal regions of the chromosomes where crossing over and ectopic exchanges are reduced. For all chromosome arms but 3L, however, the TE site density increases from the proximal to the distal parts of the chromosomes (the centromeric regions were excluded in this analysis) with sometimes a sharp decrease in density at the extreme tip, following in part the exchange coefficient. The way the copy number of TEs is contained in genomes depends thus on the element considered, and on various forces acting simultaneously, indicating that models of TE dynamics should include details of each element.
High rate of movement of one (mdg3) out of four transposable elements in a natural population of Drosophila melanogaster.
We have analysed by in situ hybridization the insertion site polymorphism of the copia, mdg1, mdg3, and P transposable elements in diploid genomes of wild males from a natural population of Drosophila melanogaster. The values of observed average degree of individual heterozygosity for all elements except mdg3 deviate statistically from the values expected by site frequencies, revealing a tendency toward homozygosity as if drift or a structuration in the population was operating. The high degree of heterozygosity for mdg3 which in addition does not deviate from the expected value, suggests that recent high rate of movements has strongly countered the population host effect on this element. Hence, heterozygosity for TEs may well reflect the history of the population, and reveals temporary high rate of site movement existing in natural populations.
Multiple amylase genes in Drosophila ananassae and related species.
The number and organization of amylase genes in Drosophila ananassae were investigated through classical genetic methods and in situ and filter hybridizations. At least four genes may be active in D. ananassae, organized as two independent pairs of closely linked copies on the 2L and 3L chromosomal arms. Several other species of the D. ananassae subgroup were studied and show the same chromosomal locations, suggesting an ancient duplication event. However, the number of Amy copies seems to be higher in the D. ananassae multigene family, and there is a striking intraspecific molecular differentiation.
Mitotic and salivary gland chromosome analyses in the Musca domestica L. (house fly) (Diptera: Muscidae).
The mitotic chromosomes in Musca domestica consist of five pairs of autosomes and an X, Y sex chromosome pair. They respond to C-banding with procentric bands on all autosomes and deep staining over most of the X and Y chromosomes. Polytene chromosomes were previously found in several larval and pupal tissue of Musca domestica. Polytene chromosome reference maps of the two sexual and the five autosomal chromosomes of Musca domestica from salivary gland cells are shown. Characteristic features of each chromosome are described identifying areas that are difficult to analyse.
Genetic and molecular analyses of vgal: a spontaneous and unstable mutation at the vestigial locus in Drosophila melanogaster.
We describe herein, a new unstable mutant of the vestigial locus, isolated from a French natural population. From this mutant vestigial almost (vgal) wild-type flies (vgal+) and extreme vg phenotypes (vge) arose spontaneously without genomic shock. The occurrence of vgal+ or vge alleles depends mostly on the breeding temperature; vgal+ revertants arose principally at low temperature (21 degrees C) and vge at 28 degrees C. These events occur mainly in the male germ line and the phenomenon appears to be premeiotic. Our results with in situ hybridization experiments and Southern blots show that the vgal mutation is due to a 2 kb DNA insertion, which is a deleted hobo element. Genetic and molecular analyses show that two distinct events may underly the wild-type revertants. One is the excision of the resident hobo element, the other a further deletion (about 300 bp in the example characterized herein). The vge mutation is probably due to a deletion of vestigial sequences flanking the hobo insertion.
Active mariner transposable elements are widespread in natural populations of Drosophila simulans.
The occurrence of active, or autonomous, mariner elements was investigated by crossing white-peach mutant Drosophila simulans females with wild-type males from various geographic origins. From a total of 194 experimental crosses only 17 failed to produce progeny with eye mosaicism (MOS, i.e. pigmented spots in otherwise white-peach eyes). Therefore, active mariner elements inducing somatic excision of the copy inserted at the white locus are abundant in all populations sampled. In the experimental crosses the frequency of mosaic offspring ranged from 0 to 100%, showing that the phenotypic expression is highly variable. The MOS phenotype, measured by the number of spots on the eyes, is quite variable within the progeny of single crosses. Although a difference was observed in the average MOS score (percentage of mosaic flies) between northern and southern populations of France, there was no indication of long range variation between geographic populations. Neither was there a systematic difference between recently collected populations and samples kept several years as isofemale lines.
Abnormal genetic segregation associated with the presence of a Y. w+ chromosome in Drosophila melanogaster.
We have observed an abnormal genetic segregation in the progeny of crosses between males of the F71 (y wa/Y.w+) strain and females of various strains carrying marker mutations on their chromosome 2. The Y.w+ chromosome, previously described as possibly being associated with a translocation of the 22D region of chromosome 2, was shown to carry the 21A1-22E4 tip of the 2L chromosome. One chromosome 2 of F71 had a deletion of this region. The abnormal genetic segregation observed in the progeny of different crosses can be explained both by the partial lethality (which becomes severe in some homogeneous genetic backgrounds) due to trisomy of the 21A1-22E4 chromosome 2 fragment and by the lethality associated with monosomy of this 21A1-22E4 segment.
Distribution and conservation of the foldback transposable element in Drosophila.
Foldback elements are a family of transposable elements described in Drosophila melanogaster. The members of this dispersed repetitive family have terminal inverted repeats that sometimes flank a central region. The inverted repeats of all the family members are homologous. The study of the distribution and conservation of the foldback elements in different Drosophila species shows that this distribution is different from that of the hybrid dysgenesis systems (PM and IR). Sequences homologous to foldback elements were observed by Southern blots and in situ hybridization in all species of the melanogaster subgroup and in some species of the montium and takahashii subgroups. The element was probably already present before the radiation of these subgroups. No evidence of horizontal transmission of the foldback element could be observed.
Identification of left-handed Z-DNA by indirect immunofluorescence in polytene chromosomes of Chironomus thummi thummi.
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Identification of left-handed Z-DNA by indirect immunomethods in metaphasic chromosomes of a mammal, Gerbillus nigeriae (Gerbillidae, Rodentia).
Antisera and antibodies against Z-DNA were used on metaphasic fixed chromosomes of a mammal, Gerbillus nigeriae (Gerbillidae, Rodentia). By indirect immunofluorescence and indirect immunoperoxidase labelling a heavy staining was detected in a fraction of the R-band positive heterochromatic segments, which are presumed to be rich in G-C base pairs. A weak and non homogeneous staining was also observed on euchromatic segments. The presence of a left-handed Z-DNA in mammalian chromosomes is discussed.
Relationships within the melanogaster species subgroup of the genus Drosophila (Sophophora). II. Phylogenetic relationships between six species based upon polytene chromosome banding sequences.
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Relationships within the melanogaster species subgroup of the genus Drosophila (Sophophora) i. inversion polymorphisms in Drosophila melanogaster and Drosophila simulans.
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[Hybridization of a new species, Drosophila mauritiana, with D. melanogaster and D. simulans].
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Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VII. Homology of puffing patterns on chromosome arm 3L in D. melanogaster and D. yakuba, with notes on puffing in D. teissieri.
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