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Biomedical subjects

F Lee

Publications and source records attributed to F Lee.

At least 289 records · Page 16Linked to original sources
PubMed1982

Factors related to utilization of dental services by the elderly.

A household interview survey of 883 persons aged 62 and older residing in Seattle, Washington, asked about a broad range of health care and social service issues, including the need for and use of dental care. The Anderson model of health services utilization was used to identify predisposing, enabling, and need characteristics hypothesized to affect the use of dental services. A path analysis was conducted to distinguish the direct and indirect effects of the variables. The results showed that none of the predisposing variables, including age, was a significant factor in explaining the use of dental services. Education had both direct and indirect positive relationships to use. Having a regular sources of dental care was also an important factor affecting utilization. Neither income nor insurance variables were powerful factors. Need, measured by an index of dental problems and having dentures, was the stronger determinant of dental care use. In general, the model was better at predicting whether or not dental care would be sought by an older person at all (R2 = .27) than in predicting the amount of service used (R2 = .06).

Aged↗
PubMed1981

Maxillary sinus carcinoma.

Primary site control, anatomical site of failure, survival, and complications of treatment were determined in a retrospective review of primary maxillary sinus carcinoma. Sixty-one patients were treated by radiation followed by surgery and 35 by radiation alone. Primary tumor control was achieved in 69% of patients receiving combined treatment, 14% of patients treated with radiation alone, and 49% of all patients. Neck nodes were the initial site of tumor recurrence in 20% of patients treated with combined treatment. distant metastases were the first site of tumor recurrence in 8% of patients. Local control did not differ with histological type. Virtually all epidermoid and undifferentiated carcinoma recurrences occurred within 2 years, but 27% of adenocarcinomas recurred after 2 years.

Female↗
PubMed1981

Co-expression and amplification of dihydrofolate reductase cDNA and the Escherichia coli XGPRT gene in Chinese hamster ovary cells.

We have transformed Chinese hamster ovary cells with a plasmid containing mouse dihydrofolate reductase (DHFR) cDNA and the Escherichia coli xanthine-guanine phosphoribosyl transferase (XGPRT) gene under the control of the mouse mammary tumor virus and SV40 early promoters, respectively. Selection for the expression of XGPRT using the dominant selection scheme described by Mulligan and Berg yields clones that simultaneously express DHFR. Growth of these cells in progressively increasing concentrations of methotrexate, results in selection of cells that overproduce DHFR and its messenger RNA 250-500 fold. ANalyses of the plasmid DNA sequences in these cells reveal that the increased production of DHFR is due in part to gene amplification (approximately 50-fold) and in part to a selective overproduction of DHFR RNA. Last, the methotrexate-resistant cells contain 50 times more XGPRT RNA and DNA than the initial transformant; this demonstrates the potential for using gene amplification as a means for overproducing the products of genes linked to DHFR cDNA in plasmid vectors.

Animals↗
PubMed1981

Carcinoma of the oral tongue: a study of patient selection and treatment results.

Retrospective review of 118 primarily treated cancers of the oral tongue was done to study patient selection and to search for improved treatment strategies. Small surface lesions were treated by local excision (LE); most small lesions invading muscle of the tongue without lymph node metastases were treated by radiation alone (RA) while larger lesions and those with palpable nodes were treated by preoperative radiation and surgery (R + S). Ultimate control of the primary tumor and lymph nodes after initial treatment and surgical salvage was high for the lesions by LE (91%), the T1N0 lesions treated by RA (88%) and for the TxN+ lesions treated by R + S (57%). Improved treatment strategies are suggested for T2N0 lesions treated by RA because of poor tumor control (53%) and a high rate of radiation complications (25%), and for T3N0 lesions because so many of these patients died from causes other than cancer within two years. Second primary cancers were most common in those patients with a good prognosis.

Aged↗
PubMed1980

Effects of sodium arsenite on single-strand DNA break formation and post-replication repair in E. coli following UV irradiation.

A non-lethal dose of sodium arsenite is found to inhibit the formation of single-strand DNA breaks in Escherichia coli WP2 wild-type and WP6 polA strains after UV irradiation. Inhibition of single-strand breakage follows a dose-dependent relationship with respect to increasing sodium arsenite concentration. ATP level in WP2 cells is decreased in the presence of sodium arasenite and therefore the inhibition of DNA break formation may be mediated through lowered ATP levels in the irradiated cells. In the presence of a non-lethal dose of sodium aresenite, post-replication repair in WP2 uvrA strains after UV irradation is also inhibited.

Adenosine Triphosphate↗
PubMed1980

Primary cultures of feline acinar cells: dissociation, culturing, and viral infection.

A technique for dissociating and plating of feline parotid acinar cells by enzymatic dispersion with collagenase and trypsin is presented. Viability of the cultured cells was determined by: 1) estimating cellular growth with visual cell counts and by [3H]thymidine incorporation; 2) observing the lack of uptake of vital dyes by the cultured cells: 3) making light, phase contrast, and electron microscopic morphological examinations; and 4) determining the lack of amylase secretion without stimulation, and the evidence of amylase secretion in response to isoproterenol. The cultured feline parotid acinar cells were infected with feline rhinotracheitis virus, a member of the herpes group. Evidence for viral infection was morphological and by indirect fluorescent antibody studies. The viral infected cells also showed no amylase release without stimulation and demonstrated an initial response to a beta-adrenergic agonist by secretion of amylase, but repeated stimulation of the virally infected cells did not produce amylase secretion.

Amylases↗
PubMed1980

Laryngeal radiation after hemilaryngectomy.

Laryngeal radiation was administered to 30 patients after hemilaryngectomy for true vocal cord carcinoma at Washington University Medical Center during the years 1957-1976. Twelve patients were irradiated at the time of gross recurrence and 5/12 (42%) of these patients had recurrence of their disease after radiation. Eighteen patients were irradiated immediately after hemilaryngectomy because positive margins were seen in the hemilaryngectomy specimen and 5/18 (28%) of these had recurrence. All but 1 recurrence occurred within 13 months following radiation. Total laryngectomy salvaged an additional 3 patients for ultimate cancer control in 23/30 (77%). This high dose laryngeal irradiation was well tolerated. A major complication occurred in 1 patient (3%) and consisted of permanent laryngeal edema necessitating tracheostomy but permitting useful speech. Mild complications occurred in 5 patients.

Carcinoma, Squamous Cell↗
PubMed1979

Carcinoma of the supraglottic larynx.

Retrospective review of 160 patients with carcinoma of the supraglottic larynx was accomplished for the period 1964 to 1974. The majority were treated by low dose preoperative radiation and conservation surgery and the rest by preoperative radiation and total laryngectomy or radiation alone. Relapse-free and actuarial 5 year survival for all patients was 71% and 45%, respectively. Of primary and neck failures, 85% occurred within 2 years. Primary, nodal, and distant failures occurred in 11%, 21%, and 14% of all patients, respectively. Of 40 T and/or N failures, 33 (83%) occurred in the neck and three T and six N failures (23%) were surgically salvaged. Voice was preserved in 71% of the patients. Major complications were noted in 16% and minor complications in 26% of surgically managed patients; operative mortality was 4%. The presence of microscopic lymph node metastases best predicted those who would later develop T and/or N failure and distant metastases. Second primary tumors occurred in 26% and the leading cause of death was tumor.

Adult↗