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Biomedical subjects

F Koch

Publications and source records attributed to F Koch.

At least 145 records · Page 8Linked to original sources
PubMed1976

Control of peptide chain initiation in uninfected and virus infected cells by membrane mediated events.

Initiation of protein synthesis in tissue culture cells is rapidly inhibited or blocked by addition of either DMSO, ethanol, TPCK, cytochalasin B, or sucrose to the growth medium. In contrast, these agents do not interfere with the initiation of protein synthesis in cell-free extracts to a comparable extent. These results support the hypothesis that protein synthesis in tissue culture cells can be influenced by membrane mediated events. Translation of viral mRNA in RNA virus infected cells is resistant to a number of these inhibitors of peptide chain initiation and proceeds under conditions where translation of host mRNA is almost completely suppressed. It appears that viral mRNA possesses a greater ability than host mRNA to form mRNA-ribosome initiation complexes when the overall rate of peptide chain initiation is reduced. This observation has led to a number of predictions concerning the strategy of virus directed suppression of host mRNA translation. Under optimal growth conditions protein synthesis appears to be regulated mainly, but not exclusively, by the amount of the mRNA available for translation. However, when cellular growth and/or the overall rate of peptide chain initiation is restricted, control of protein synthesis at the translational level becomes decisive with the translation of each mRNA species proceeding with its own characteristic efficiency most probably as a result of inherent differential affinities of individual mRNA species for ribosomes.

Cell Line↗
PubMed1975

Ultrasonically guided percutaneous fine needle biopsy of the pancreas.

By ultrasonic scanning, it is possible with reasonable accuracy to demonstrate space occupying lesions in the pancreas, and with a delicate ultrasonic technique, a puncture needle can be guided to a predetermined mass in the pancreas. In 25 patients with suspected pancreatic lesions, scanning demonstrated a solid mass lesion in the pancreas. Twenty-one patients had carcinoma; four had chronic pancreatitis. In 17 of the 21 patients with carcinoma, tumor cells were aspirated. The cells were numerous and easily recognizable, fulfilling the usual cytologic criteria of malignant disease. In three of the 16 patients with carcinoma, the aspirations were inadequate, and one showed normal pancreatic cells. In the four patients with chronic pancreatitis, normal pancreatic cells were aspirated. There were no immediate complications due to fine needle biopsy. The combination of ultrasonic scanning and ultrasonically guided percutaneous fine needle aspiration is an important adjunct to the management of pancreatic lesions.

Amylases↗