Biomedical subjects
Publications and source records attributed to F Klein.
Erickson's utilization approach provides a model of hypnotic and strategic intervention for persons seeking psychotherapy because of sexual orientation confusion. Case studies outline examples of hypnotic and strategic short-term interventions. These cases include the use of metaphor, utilizing resistances, anchoring, and synesthesia.
The opsonic activity of five immunoglobulin preparations for intramuscular and subcutaneous substitution therapy was investigated. Various strains of Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, group B streptococci, Streptococcus pneumoniae and Haemophilus influenzae were used. In addition, the concentration of unsplit IgG molecules, IgG fragments and IgG aggregates in these preparations was determined. With the exception of H. influenzae, all preparations opsonised the bacteria studied almost as well as inactivated serum. All preparations contained an appreciable amount of aggregates, but IgG fragments were not found.
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Three laboratories took part in an investigation about the possibilities of quantitative determination of IgG subclasses. Polyclonal monospecific antisera were used in combination with different kinds of calibrators to assess the IgG subclass levels in three W.H.O. reference preparations. IgG 1 could be determined with satisfactory precision; for the other subclasses the quantification was less precise. The precision was dependent on the antisera for IgG 1, IgG 2 and IgG 4 but mainly on the calibrators for IgG 3. Mass units are to be preferred to relative units. W.H.O. reference preparation 67/97 is proposed as a reference for determination of IgG subclasses in mass units. The target values are 5.0 g/l for IgG 1, 2.6 g/l for IgG 2, 0.4 g/l for IgG 3 and 0.5 g/l for IgG 4. These values add up to a total IgG value close to earlier W.H.O. estimates.
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Theory and research concerning sexual orientation has been restricted in its scope and influence by the lack of clear and widely accepted definitions of terms like heterosexual, bisexual, and homosexual. In an attempt to better demarcate and understand the complexities of human sexual attitudes, emotions, and behavior, the Klein Sexual Orientation Grid (KSOG) was developed and administered. The KSOG is composed of seven variables that are dimensions of sexual orientation, each of which is rated by the subject as applying to the present, past, or ideal. Analysis of the data from subjects who filled out the KSOG in Forum Magazine indicated that the instrument was a reliable and valid research tool which took into consideration the multi-variable and dynamic aspects of sexual orientation.
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The results of quantification of human IgM by radial immunodiffusion (RID) are influenced by the charge of the diffusion medium and by the radio of IgM to other serum proteins. Agarose has almost no influence, while some heavily charged agars are unsuitable for RID, even after reduction of the IgM. Reference and test sera should not differ too widely in IgM levels. The interaction between charged agar and human IgM explains the subgroup phenomenon described earlier by Klein et al. (1973).
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The technicalities of the UNIVERSAL operation unit, the UNIMOBIL chairstand and the UNISTAT microscope stand are described.
Sendai and Newcastle disease viruses were tested for their induction potential in the production of human lymphoblastoid interferon [HuIFN-alpha(Ly)] from Namalva cell cultures. Tests were performed at multiple induction levels, for two induction periods, and on primed and nonprimed cell cultures. Sendai virus proved statistically more effective overall; priming and induction period had no significant effect.
In this study 12 commercial immunoglobulin preparations for intravenous administration were investigated with respect to their opsonic activity for various strains of Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, and group B streptococci, and in addition, the concentration of unsplit IgG molecules, IgG fragments, and IgG aggregates (dimers) was determined. The results show that four preparations that had been treated with enzyme or by reduction and alkylation had hardly any opsonic activity; the other preparations were as effective as inactivated serum. A statistical analysis showed an excellent correlation between the concentration of intact IgG molecules in a preparation and the opsonic activity for the various bacteria; a negative correlation between the concentration of IgG fragments and the opsonic activity was found. The combination of the two techniques used in this study provides a basis for recommendations on the choice of gammaglobulin preparations for clinical use.
A pilot-plant-scale operation was used for studying membrane ultrafiltration and concentration of kiloliter quantities of the lymphokine interleukin-3 with a single set of membranes. Initial use of ammonium sulfate precipitation of interleukin-3 proved erratic in the recovery of biological activity and resulted in corrosion of the processing equipment. Membrane ultrafiltration proved to be effective in enabling control of the degree of concentration and predicting recovery of the biologically active protein.
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In 49 patients undergoing open-heart surgery we compared the electroencephalographic (EEG) effects of high-dose morphine, fentanyl, or sufentanil with O2, using two computerized analysis and display techniques: a period analysis (the Klein method) and an aperiodic analysis (the Neurometrics monitor). During fentanyl or sufentanil anesthesia, both techniques revealed a general decrease in frequency, shown by the aperiodic analysis primarily as a marked increase in the very low frequency range: an increase in the 1-Hz bin (TP1, in muv2) from 2.80 X 10(4) +/- 3.20 X 10(4) (SD) to 45.1 X 10(4) +/- 27.2 X 10(4) for fentanyl and from 3.11 X 10(4) +/- 2.83 X 10(4) to 52.8 X 10(4) for sufentanil. The cumulative percent power at 3 Hz (CP3) increased from 27.2 +/- 6.8 to 83.0 +/- 11.0 for fentanyl and from 22.7 +/- 5.2 to 85.1 +/- 10.4 for sufentanil, while the frequency at 90% cumulative percent power (F90, in Hz) decreased from 17.8 +/- 2.9 to 7.9 +/- 2.8 for fentanyl and 16.4 +/- 5.2 to 5.6 +/- 4.3 for sufentanil. The changes with morphine were less obvious, with some attenuation of high-frequency power shown by the Klein method, and an increase from 24.1 +/- 8.6 to 59.3 +/- 20.7 with CP3, but no change in TP1. Low-frequency power with the period analysis and TP1 with the aperiodic analysis decreased between laryngoscopy and the incisions with fentanyl and sufentanil.(ABSTRACT TRUNCATED AT 250 WORDS)
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The authors try to draw conclusions from Winnicot's articles on the discontinous nature of emotional development. They try to understand how autism may originate in disruptions of self-non-self" attachments. However, they admit that the child's personality may develop even within such disruptions. In an experiment of intensive care in a day-hospital for autistic or psychotic children they have tried to restore these attachments in the hospital itself, through continuous care by each member of the staff, regardless of qualification or training. This continuity provides a basis for relationships which may be voiced and interpreted during the children's therapy as they share this reference experience. Its continuity makes life in the institution therapeutic in itself, even without psychoanalytic interpretations. The lack of spoken language gives rise to communication difficulties and use of an educated self is a current project. Two clinical observations exemplify the parallel work of parents and children and their reciprocal interactions. As the origin and future of these psychoses are still open questions, the possible responsibility of predisposing or initial fragilizing factors which facilitate this lasting personality organization is to be considered.