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Biomedical subjects

F Keller

Publications and source records attributed to F Keller.

At least 343 records · Page 19Linked to original sources
PubMed1981

[Investigation of the viability of radioactively labelled bacteria after adsorption on activated charcoal (author's transl)].

A method for radioactive labelling of bacteria is described. [35S]-L-methionine labelled Staphylococcus aureus and Escherichia coli used as model microorganisms were adsorbed onto the surface of acrylhydrogel coated charcoal. On the average 33% of S. aureus and 10% of E. coli supplied were bound. In both cases most of the pathogens (75-90%) could be desorbed by intensive washing, but about 3% of the bacteria provided remained fixed to the surface of the charcoal. Of these remaining bacteria 50% (in the case of S. aureus) or 1% (in the case of E. coli) could be detected in subsequent cultures. These experiments demonstrate that a modified technique of haemoperfusion may be useful in the diagnosis of septicaemia. Haemoperfusion charcoal in an extracorporal circulation of a patient serves as a matrix for adsorption of microorganisms from the circulating blood. This leads to an "in vivo" enrichment of circulating pathogens which may be cultured, identified and their antibiotic sensitivity tested in the usual manner. Thus, this procedure may provide a new diagnostic tool in the diagnosis of septicaemia (9).

Adsorption↗
PubMed1981

[An improved method for selective determination of serum albumin by the bromcresol green method, using the SMAR 12/60 (author's transl)].

The estimation of serum albumin obtained with SMAR 12/60 by the bromcresol green method are in good agreement with values determined by electrophoresis only in the normal range. Interestingly marked discrepancies occur in the clinically relevant cases of hypalbuminosis. The shortening of the reaction time between bromcresol green and serum results in a better agreement between the values obtained with SMAR 12/60 and the values determined by electrophoresis over the whole range.

Autoanalysis↗
PubMed1981

[Polarization microscopic studies of the fine structure of the maternal-fetal barrier in normal human placentas depending on gestational age].

The submicroscopic structure of the throphoblast basement membrane and the collagen in the chorionic villi of human placentas was investigated by polarization microscopic technique. The study establishes a quantitatively measurable difference in the submicroscopic range of the placental membrane in relation to histologically classified disturbances of maturity.

Basement Membrane↗
PubMed1980

[Improvement of blood viscosity in sickle-cell anaemia by pentoxifylline (author's transl)].

An 18-yer-old girl with homozygotic sickle-cell anaemia (HbSS: HbS 65% and HbF 4.9%) suffered from painful haemolytic crises since six years of age, these crises occurring almost weekly of late. Pentoxifylline (2.4 g daily in three divided doses) decreased blood viscosity in vitro from 6.7 to 4.9 (normal 4.3-5.3 at 46 s-1 shear rate) and erythrocyte filtration accelerated from 127 to 77 s (normally 30-49 s). Although pentoxifylline could not prevent haemolysis, previously necessary analgesics were no longer required, even during haemolytic crises. Thus the drug lowers viscosity also in vivo and thus improves microcirculation. It has a prophylactic effect against vaso-occlusive complications of sickle-cell anaemia.

Adolescent↗
PubMed1980

Digoxin dosage in renal insufficiency: impracticality of basing it on the creatinine clearance, body weight and volume of distribution.

Previous dosing schedules for digoxin in renal failure have considered the decrease in the elimination rate constant but not the decrease in the volume of distribution. A dosing schedule based on the creatinine clearance, body weight and volume of distribution has been developed from pharmacokinetic data taken from the literature. Its validity was tested in a clinical study of 35 patients with chronic renal insufficiency not requiring dialysis. The dosing schedule resulted in correct digitalization expressed as a steady state plasma digoxin concentration in the therapeutic range (0.5-2.0 ng/ml) in 25 out of 27 patients (93%). However, of 82 possible candidates for the study, it could not be performed in 47 (57%). The high drop-out rate was mainly due to the complicated dosing schedule and to the difficulty of repeatedly measuring creatinine clearance on a routine basis. Therefore, safe dosing of digoxin in renal insufficiency does not seem to be feasible in practice. Digitoxin may be a better alternative.

Adult↗
PubMed1979

[Worldwide eradication of smallpox or from Sarah Nelmes to Ali Maow Maalin].

It cannot be disputed that the WHO plan for the eradication of smallpox has had considerable success. There is no endemic smallpox any longer in the world. Nevertheless caution is advised and continued supervision must not be neglected. There are still countries where supervision is not satisfactory, there is uncertainty about animal reservoirs and it should be assumed from the beginning that up to the present no epidemic disease could be completely eradicated. For this reason it is inevitable that supplies of vaccine should be available. A vaccine from heat sensitive strains is offered which is obtained from cell cultures.

Animals↗
PubMed1979

Effect of cell-mediated immune factors on the replication of an attenuated temperature-sensitive mutant of vaccinia virus.

Studies of the in vivo multiplication of an attenuated temperature-sensitive strain of vaccinia virus (ts2) indicated that temperature sensitivity alone could not account for the attenuation. Immunodepressive treatment of intracerebrally inoculated mice had a dramatic stimulatory effect on the multiplication of the attenuated strain and suggested that establishment of ts2 infection in the mice was hindered by host defense mechanisms mediated by cellular elements. Experiments carried out in vitro with the 51Cr release assay showed that cells infected with the ts2 mutant represented more susceptible targets to the cytotoxic action of immune spleen cells than cells infected with the parent vaccinia strain. Moreover, it appeared that ts2 replication, compared with wild-type replication in vitro, was more inhibited by the immune spleen cells. Although this work did not evaluate the role of specifically sensitized lymphocytes within the lesions, indirect evidence suggests that lymphocytes could cause a more effective halt in ts2 virus multiplication than in wild-type multiplication.

Animals↗
PubMed1979

[Coronary vessel anomaly: fistula between the right coronary artery and the left ventricle clinically presenting as aortic valve insufficiency (author's transl)].

Under more than 200 cases of coronary vessel anomalies only 33 are fistulas from the right or left coronary artery into the left ventricle. The case presented here of a fistula from the right coronary artery into the left ventricle featured the clinical picture of an aortic valve insufficiency. The histologic findings support the theory that these fistulas are embryological anomalies: transient communications between the ventricular trabeculas and the coronary vessels may be lined with endothelium and become then persisting as fistulas.

Aortic Valve Insufficiency↗
PubMed1979

Amelioration of blood viscosity in sickle cell anemia by pentoxifylline. A case report.

An 18 year old female patient with homozygous sickle cell anemia (HbSS: HbS 65% and HbF 4.9%) has been suffering from painful hemolytic crises about once a week since her childhood. Under treatment with pentoxifylline (2.4 gm daily in three divided doses), the blood viscosity decreased from 6.7 to 4.9 cP (normal: 4.3 to 5.3 cP at shear rate 46/sec) and the erythrocyte filtration accelerated from 127 to 77 sec (normal: 30 to 49 sec). Though hemolysis could not be prevented by pentoxifylline, there was no further demand for analgesic medication even in the hemolytic state. By decreasing blood viscosity and improving microcirculation, pentoxifylline may prevent the vasoocclusive complications of sickle cell anemia.

Adolescent↗