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Biomedical subjects

F Kalousek

Publications and source records attributed to F Kalousek.

59 records · Page 4Linked to original sources
PubMed1970

Dissociation of 70 S E. coli ribosomes induced by a ribosomal factor (DF). Electrophoretic studies of the ribosomal particles.

The dissociation of purified 70 S.E. coli ribosomes, induced by the dissociation factor DF, has been studied by submitting the reaction mixtures to electrophoresis on polyacrylamide gels. The electrophoretic analysis of the ribosome mixtures revealed a heterogeneity which escaped detection by conventional sucrose gradient centrifugation. Increasing amounts of DF in the reaction mixtures converted 70 S ribosomes to particles (designated 70 S (I)) which migrate slower in the electric field than the original 70 S ribosomes. These 70 S (I) ribosomes still consist of both subunits. They dissociate upon further raising the DF concentration.

Journal Article↗
PubMed1969

Deoxyribonucleic acid methylase activity in pea seedlings.

Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30 degrees C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.

Chromatography, Paper↗