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Biomedical subjects

F Josso

Publications and source records attributed to F Josso.

At least 37 records · Page 2Linked to original sources
PubMed1976

Factor V and VIII activation "in vivo" during bleeding. Evidence of thrombin formation at the early stage of hemostasis.

Factor V and VIII activity was measured by a one-stage assay on capillary blood issuing from a skin cut. This activity increases during bleeding. The phenomenon is suppressed by a previous injection of a low dose of heparin. These data give evidence of a very early local formation of thrombin during hemostasis. The study of this activation process in patients with congenital deficiency of blood clotting factor allows to investigate the mechanism of thrombin formation in vivo. This demonstrates the preeminence of the tissue factor pathway and the need of factor VIII for factor X activation in the extrinsic system.

Adult↗
PubMed1975

The procoagulant factor of leukaemic promyelocytes: demonstration of immunologic cross reactivity with human brain tissue factor.

A procoagulant activity was found in the immature cells from patients with acute promyelocytic leukaemia. It was demonstrated that this activity was related to tissue factor. The protein component of tissue factor from brain extract was purified by Nemerson's technique and injected into rabbits to obtain anti-TF antibodies. Similar antibodies were produced against the promyelocyte extract. The anti-brain tissue factor antibodies neutralized the tissue factor activity of promyelocyte extract, and antibodies against immature cells were able to neutralize the tissue factor activity of human brain extract. In immunoprecipitation studies a reaction of partial identity appeared between one component of promyelocyte extract and one component of brain tissue factor. The data demonstrated that the promyelocyte procoagulant is antigenically related to brain tissue factor.

Antibodies↗
PubMed1975

Tissue factor activity of cultured human lymphocytes.

Potent procoagulant activity develops in human lymphocytes during mixed culture, preceding the cell multiplication. This activity has the same characteristics as that of tissue factor. The membrane support of the activity binds factor VII, leading to the formation of factor X activator. The same observation is made when lymphocyte culture is made without antigenic stimulation, demonstrating that "in vitro" culture alone is able to endow lymphocytes with tissue factor activity. The mechanism of this non-specific stimulation remains to be elucitaded.

Blood Coagulation↗